FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146823
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT1946813.25950293891284No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT1781512.133657533220273No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT118968.102272804669568No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGGGACCTTCCTCTCAGAACCCCT110827.547863754316422No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCTGGTCATCCTCTCAGACCAGCT99776.795256873923022No Hit
TTGGTCCGTGTCTCAGTACNAATGTGGGGGACCTTCCTCTCAGAACCCCT63984.357627892087752No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT51153.483786600192068No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT46043.1357484862725866No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT44773.049249776942305No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT40832.780899450358595No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT39892.7168767836101972No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGCCGGCT28241.9234043712497362No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCACCCTCTCAGGTCGGCT27201.852570782506828No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT24421.663227151059439No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGTCGGCT23831.6230427112918273No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT23621.6087397751033559No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT22261.5161112359780144No Hit
CTGGTCCGTGTCTCAGTACNAGTGTGGGGGGTTAACCTCTCAGTCCCCCT12660.8622627245050163No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGGGACCTTCCTCTCAGATCCCCT8260.5625821567465588No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCTGGTCATCCTCTCAGATCAGCT7920.5394250219652235No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT6860.46722924882341327No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT5520.37596289409697387No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCT5160.35144357491673645No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT4630.3153456883458314No Hit
TTGGTCCGTGTCTCAGTACNAATGTGGGGGACCTTCCTCTCAGATCCCCT4570.3112591351491252No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGTCGGCT4480.30512930535406574No Hit
CTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT4240.28878309256724083No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCACCCTCTCAGGTCGGCT3800.2588150357913951No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGGTTAACCTCTCAGTCCCCCT3550.24178773080511912No Hit
TTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT3080.20977639743092022No Hit
TTGGGCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT2980.20296547543640983No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGCCGGCT2830.19274909244464425No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCTGGTCGTCCTCTCAGACCAGCT2800.19070581584629112No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGAAAACCTCTCAGTCCCCCT2780.18934363144738903No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGGTCAACCTCTCAGTCCGGCT2780.18934363144738903No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT2720.1852570782506828No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT2690.18321380165232967No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGTCGGCT2610.17776506405672135No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCGCCCTCTCAGGTCGGCT2550.17367851086001512No Hit
CTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT2430.16550540446660264No Hit
TTGGGCCGTGTCTCAGTACNAATGTGGGGGACCTTCCTCTCAGAACCCCT2370.1614188512698964No Hit
TTGGACCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT2360.16073775907044535No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGCGACCTTCCTCTCAGAACCCCT2340.15937557467154329No Hit
CTGGACCGTCTCTCAGTTCNAATGTGGCCGATCACCCTCTCAGGTCGGCT2340.15937557467154329No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT2220.1512024682781308No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCCGCT2210.15052137607867977No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCACCCTCTCAGGCCGGCT2210.15052137607867977No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTTCGGCT2020.13758062428911No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGCCGGCT1940.1321318866935017No Hit
TTGGTCCGTGTCTCAGTACNAATGTGGGGGGTTAACCTCTCAGTCCCCCT1870.12736424129734442No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTGAACCTCTCAGTCCCCCT1870.12736424129734442No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCCGGTCACCCTCTCAGGTCGGCT1830.12463987249954026No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTTCGGCT1820.12395878030008922No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT1810.12327768810063819No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGCCAACCTCTCAGTCCGGCT1730.11782895050502985No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT1680.11442348950777467No Hit
CTGGTCCGTGTCTCAGTACNAGTGTGGGGGGAAAACCTCTCAGTCCCCCT1670.11374239730832363No Hit
TTGGTCCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT1670.11374239730832363No Hit
TTGGACCGTGTCTCAGTTCNAGTGTGGCTGGTCATCCTCTCAGACCAGCT1650.11238021290942155No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT1640.11169912070997051No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTAGAGTCTCTCAACTCGGCT1620.11033693631106842No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCTGGTCATCCTCTCAGACCCGCT1580.10761256751326427No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCATCCTCTCAGACCGGCT1530.10420710651600908No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCGGACCTTCCTCTCAGAACCCCT1510.102844922117107No Hit
TTGGACCGTGTCTCAGTACNAATGTGGGGGACCTTCCTCTCAGAACCCCT1500.10216382991765595No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGGTCATCCTCTCAGACCAGCT1500.10216382991765595No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGGTCATCCTCTCAGACCAGCT1470.10012055331930284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGACC37950.0292.322541
CGAGTCT157.495428E-6292.322547
CACGGCG108.6916453E-4292.32254295
GTGTCTC136300.0292.000858
GGACCGT69000.0291.89893
TGGACCG69200.0291.68892
CCGTGTC136400.0291.679636
TGTCTCA136400.0291.679639
CGTGTCT136400.0291.572457
GACCGTG67950.0291.462134
ACCGTGT67900.0291.461525
TGGTCCG26500.0291.219452
GTCCGTG26450.0291.217354
TTGGTCC21350.0290.953371
CTGGACC31000.0290.90811
GTATCTC9100.0290.716378
GGTCCGT26600.0290.124633
GCCGTGT42400.0289.564765
CTGGGCC23100.0289.158871
CGTATCT9150.0289.127787
Produced by FastQC (version 0.11.5)