FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122506
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG1298610.60029712830392No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA97047.921244673730267No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGAG77806.350709353011282No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG72755.938484645650009No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCAAAGAG61425.013631985372145No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA34552.8202700275904857No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC33932.76966026153821No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGG32942.6888478931644166No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA30832.5166114312768353No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT29442.4031476009338317No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA26712.1803013729939758No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT24241.978678595334106No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG23651.9305176889295217No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGGAAGG20611.6823665779635282No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA20411.6660408469789234No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT19441.5868610517035902No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAACC19031.5533933031851501No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAAG17261.4089105839713973No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGTT16971.3852382740437204No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAACGGA13191.076681958434689No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGTGGGGCAGCGGGG10910.8905686252101938No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTC10710.874242894225589No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTG9370.7648604966287366No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGTACGCGAAAGGGA9020.736290467405678No Hit
GATGAACGCTAGCTATAGGCTTAACACATGCAAGTCGAGGGGCAGCATCA7540.6154800581196023No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG7260.5926240347411555No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGG7010.5722168710103995No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG6780.5534422803781038No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG6660.543646841787341No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAATTT6120.499567368128908No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTC6060.4946696488335265No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGAG5900.48160906404584264No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGATTT5710.4660996196104681No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAAGGGACCT5700.4652833330612378No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA5640.46038561376585635No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTCGTTT5630.45956932721662613No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGTACGAAGCGGCCC5570.45467160792124467No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT5550.4530390348227842No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGAATTTT5470.44650874242894223No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA5030.4105921342628116No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTCT4990.4073269880658907No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGAATCTTT4490.3665126606043786No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAT4270.3485543565213133No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCATCATGA3770.30774002905980113No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAATGTC3430.2799862863859729No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGTACGCGAAGGCCT3410.27835371328751246No Hit
AACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGAGAGAATTG3390.2767211401890519No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGCTTA3210.2620279823029076No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGTGAGAGA3090.2522325437121447No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATCA3060.249783684064454No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGT3000.24488596476907257No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTT2990.24406967821984227No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG2720.22202994139062576No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAATATT2680.2187647951937048No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA2500.20407163730756045No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTATTT2420.1975413449137185No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGTGAAAGTCC2300.1877459063229556No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG2270.1852970466752649No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAATC2260.18448076012603465No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAGTTA2060.1681550291414298No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC2060.1681550291414298No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATATT2050.16733874259219958No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA2050.16733874259219958No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTCAAGA1990.16244102329681812No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCAAAGAG1990.16244102329681812No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAC1990.16244102329681812No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG1980.16162473674758787No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT1860.15182929815682497No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGAAG1850.15101301160759473No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAGTCAT1820.148564151959904No Hit
GATGAACGCTGGCGGCGTGCCTAAGACATGCAAGTCGAACGCTTCACTTC1810.14774786541067378No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGAGAGAAGAG1800.1469315788614435No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTTC1760.14366643266452256No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA1680.13713614027068063No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTGCTTGA1660.13550356717222015No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAAGTCTTTA1470.11999412273684554No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACATGT1470.11999412273684554No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTATTT1460.11917783618761531No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATTACT1460.11917783618761531No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAATC1370.11183125724454314No Hit
GACGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAACC1340.1093823975968524No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTT1260.10285210520301047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAG53450.0295.470986
CAAACGC108.4154966E-4295.470953
GACAAAC108.4154966E-4295.470951
TGAACGC83450.0295.293953
CGCTAGC53450.0295.194587
CGCTGGC68650.0295.040567
CTGGCGG68650.0294.98869
CGTAGAA108.4567966E-4294.98856295
GGGCCAA108.4567966E-4294.98856295
ACTCAGA108.4567966E-4294.98856295
AACGCTG68850.0294.18355
ACGCTGG68850.0294.18356
CTAGCTA34100.0294.12359
AACGCTA53750.0294.096685
ATGAACG82100.0293.491582
CTAGCGA15300.0293.060529
GCTAGCG19650.0292.736768
ATAAACG3350.0291.060972
ACGAACG35900.0290.532752
GCTAGCT34550.0289.865758
Produced by FastQC (version 0.11.5)