FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005103555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137274
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT121708.865480717397322No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT105197.662776636508006No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT98577.180529452044815No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT77325.632530559319317No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGTTCATCCTCTCAGACCGGCT73505.354254993662311No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT69235.043198274982881No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCTGGTCATCCTCTCAGACCAGCT57854.214199338549179No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCACCCTCTCAGGTCGGCT56974.150093972638665No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT49973.6401649256232065No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT46853.4128822646677452No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT46353.3764587613094976No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGCCGGCT45043.28102918251089No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGTTCATCCTCTCAGACCGGCT40452.946661421682183No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT31182.2713696694202836No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT27712.018590556114049No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGGGACCTTCCTCTCAGAACCCCT26911.9603129507408539No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGTCGGCT26741.94792895959905No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGTCGGCT17571.2799219080088No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCACCCTCTCAGGTCGGCT11400.8304558765680318No Hit
CTGGACCGTATCTCAGTTCCAGTGTGTCCGATCAGCCTCTCAGCTCGGAT9330.6796625726648892No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT8100.5900607544036015No Hit
CTGGGCCGTGTCTCAGTCCCAATGCGGCCGATCAACCTCTCAGTTCGGCT6700.48807494500050996No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT6630.48297565453035535No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCCGGTCACCCTCTCAGGTCGGCT6540.47641942392587083No Hit
CTGGACCGTATCTCAGTTCNAGTGTGTCCGATCAGCCTCTCAGCTCGGAT5950.4334396899631394No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT5170.37661902472427405No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGGTCAACCTCTCAGTCCGGCT5060.36860585398545975No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTTCGGCT4870.35476492270932586No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT4520.329268470358553No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCTGGTCATCCTCTCAGATCAGCT4330.3154275390824191No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT4310.3139705989480892No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT4220.30741436834360475No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT4100.2986727275376255No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCGCCCTCTCAGGCCGGCT4000.2913880268659761No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCACCCTCTCAGGCCGGCT3810.2775470955898422No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCAACCTCTCAGTCCGGCT3740.27244780511968764No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGTCGGCT3730.2717193350525227No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT3680.268076984716698No Hit
CTGGGCCGTGTCTCAGTCCNAATGCGGCCGATCAACCTCTCAGTTCGGCT3610.26297769424654344No Hit
CTGGGCCGTGTCTCAGTCCCAATCTGGCCGGTCGGTCTCTCAACCCGGCT3350.24403747250025498No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT3240.23602430176144062No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGTTCATCCTCTCAGATCGGCT3190.23238195142561593No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGCCGATCACCCTCTCAGGTCGGCT3010.219269490216647No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGCCGGCT2800.20397161880618325No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGCCGGCT2760.2010577385375235No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGCCAACCTCTCAGTCCGGCT2750.20032926847035853No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT2680.19522997800020397No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGGACATCCTCTCAGACCGGCT2560.1864883371942247No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCT2550.18575986712705975No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCAGCCTCTCAGCTCGGCT2450.17847516645541034No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT2250.16390576511211152No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCACCCTCTCAGGTCGGCT2230.16244882497778165No Hit
CTGGTCCGTGTCTCAGTACNAGTGTGGGGGGTTAACCTCTCAGTCCCCCT2230.16244882497778165No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACTCTCTCAAGCCGGCT2200.16026341477628686No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT2160.15734953450762706No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT2100.15297871410463743No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCGCCCTCTCAGGCCGGCT2090.1522502440374725No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTTCGGCT2060.15006483383597768No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGGGACCTTCCTCTCAGATCCCCT1870.13622390255984382No Hit
TTGGTCCGTGTCTCAGTACNAATGTGGGGGACCTTCCTCTCAGAACCCCT1750.12748226175386454No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCAACCTCTCAGCTCAGCT1700.12383991141803982No Hit
ATGGGCCGTGTCTCAGTCCCATTGTGGCTGATCATCCTCTCAAACCAGCT1700.12383991141803982No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGGACATCCTCTCAGACCGGCT1660.12092603114938008No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCAACCTCTCAGTCCGGCT1630.11874062094788525No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGATCAGCCTCTCAGCTCGGCT1600.11655521074639044No Hit
CTGGGCCGTGTCTCAGTCCNAATCTGGCCGGTCGGTCTCTCAACCCGGCT1570.11436980054489561No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCTGGTCGTCCTCTCAGACCAGCT1540.11218439034340079No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCCGATCACCCTCTCAGGTCGGCT1480.10781356994041115No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGCCAACCTCTCAGTCCGGCT1450.10562815973891634No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT1450.10562815973891634No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCAACCTCTCAGTTCGGCT1410.10271427947025656No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)