FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132692
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA107308.086395562656378No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAGGAAAA99547.501582612365477No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT65344.924185331444247No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA59314.469749495071293No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA47073.547312573478431No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG46613.5126458264251053No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG44873.3815150875712177No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA37022.7899195128568413No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGGAAGG33392.516353661109939No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGG32992.4862086636722633No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGAGAGAAGAG29792.245048684170862No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGAACTTA27902.1026135712778466No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT26191.973743707231785No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCTTGAT24921.8780333403671663No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAACC21451.6165254875953334No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG19731.48690199861333No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT18481.3926988816205952No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTGTTT12490.9412775449914087No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT10580.7973351822265095No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA10460.7882916829952069No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAACCACTTC9350.7046393151056582No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCTGC9240.6963494408102976No Hit
AACGAACGCTGGCGGCAGGCTTAACACATGCAAGTTGAACGGGATTTGTA8910.6714798179242155No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGATTCTCTTC8550.6443493202303078No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT8550.6443493202303078No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTCAAGA7890.5946100744581436No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTCGTTT7890.5946100744581436No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCTATC7570.5704940765080035No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA7200.5426099538781539No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGAG7130.5373345793265607No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTATTT6980.5260302052874325No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGAATCTTT6910.5207548307358394No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT6820.5139722063123625No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG6760.5094504566967112No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGTGAATCT6560.4943779579778736No Hit
GACAAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGATTGCG6420.4838272088746872No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAATGAAGTT6410.4830735839387454No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC6110.4604648358604889No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTGAAGCT6030.45443583637295387No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTGTGT5870.44237783739788383No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTA5690.42881258855092996No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG5560.41901546438368553No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTCT5270.39716034124137095No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGATAAGC5110.3851023422663009No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGTTT5070.38208784252253336No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA4950.3730443432912308No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTTC4720.35571096976456756No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGTGAGAGA4400.3315949718144274No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT4300.3240587224550086No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGCAAATAC4130.3112470985439966No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGAAGCTTTCT4130.3112470985439966No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT3890.2931601000813915No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTG3680.277333976426612No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGATGAAACCG3660.27582672655472823No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCGATTT3400.25623247822023937No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGGAA3240.24417447924516927No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAATCAAGA3160.2381454797576342No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCCTT3110.23437735507792481No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA3090.23287010520604107No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAGGAAAA3060.2306092303982154No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA2930.22081210623097097No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCACGA2920.2200584812950291No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTC2890.21779760648720348No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA2890.21779760648720348No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCCACC2840.21402948180749404No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGCTT2760.208000482319959No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAGTTA2740.20649323244807524No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG2690.20272510776836583No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAACTATTT2530.19066710879329576No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA2440.18388448436981883No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGATTT2390.18011635969010942No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATAAGC2370.17860910981822567No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTT2340.17634823501040003No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGG2240.1688119856509812No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGTGAAAGTCC2220.16730473577909746No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG2220.16730473577909746No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACCTT2180.16429023603532994No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGGTTT2070.15600036173996926No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATTACT2040.15373948693214362No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAATC2020.15223223706025985No Hit
ATTGAACGCTGGCGGAACGCTTTACACATGCAAGTCGAACGGTAACGCGG2000.1507249871883761No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGAGGAAGG1970.14846411238055046No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGAGA1930.1454496126367829No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTCGGCC1920.14469598770084105No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGTT1880.14168148795707353No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTCT1880.14168148795707353No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTTTAT1830.13791336327736411No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT1810.13640611340548037No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTACAT1790.13489886353359662No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATATT1700.12811623911011968No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA1640.1235944894944684No Hit
GACGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGGAAGG1630.12284086455852652No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATTATT1630.12284086455852652No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACATGT1620.12208723962258464No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCCCTC1600.12057998975070086No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCGGAT1600.12057998975070086No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGAGTTAT1590.11982636481475899No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGGGAAAGAT1570.11831911494287523No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTATTT1510.11379736532722395No Hit
GACGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAACC1510.11379736532722395No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGATCTTCTTC1500.11304374039128207No Hit
GATAAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGTTTTTC1490.11229011545534019No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCACT1470.11078286558345643No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTAACCCG1460.11002924064751456No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATACT1430.10776836583968889No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATAAGT1420.10701474090374702No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGAGCGAT1410.10626111596780513No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGATTT1400.10550749103186326No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGG1390.10475386609592138No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTT1380.1040002411599795No Hit
ATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGGTAACATAA1380.1040002411599795No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGCTAGGA1370.10324661622403762No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTGAAA1370.10324661622403762No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACATT1360.10249299128809575No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG1350.10173936635215386No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGTGAATTT1330.1002321164802701No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACGC500.0295.434883
ACGCTAG23750.0295.434886
ATAAACG3950.0295.434882
CGCTGGC108300.0295.298467
ACGCTGG108400.0295.026066
TAGGCCC108.457511E-4294.98944295
CAGACAC108.457511E-4294.98944295
TGAACGC61100.0294.951353
CTGGCGG108500.0294.445689
GCTGGCG108450.0294.445438
CTAGCTA11400.0293.695629
CGCTAGC23900.0293.58077
AACGCTG109200.0293.05
GATAAAC4000.0291.741941
GCTAGCT11500.0289.85928
GACGAAC67050.0289.26621
GAACGCT130950.0287.651374
CGAACGC69100.0284.746223
AAACGCT4650.0282.7284
TAAACGC4150.0281.197053
Produced by FastQC (version 0.11.5)