FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100295
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAGGAGAA1272012.682586370207888No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGACA84668.441098758661948No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAC45984.584475796400618No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGATA43754.3621317114512195No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA38433.831696495338751No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT24772.4697143426890675No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTCT20562.049952639712847No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTA19891.983149708360337No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGCGAAAGTGG19701.9642055934991773No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGAATCTTCTT19611.9552320654070494No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG17161.7109526895657807No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA14351.430779201356No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGCGG13961.3918939129567776No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG13721.3679645047111022No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGTA12821.27822922378982No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGCGGGGCAGCATGT12721.2682586370207887No Hit
GATAAACGCTGGCGGCGTGCCTAATACATGCAAGTCGATCGATAGAAGTC12161.212423351114213No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT11191.1157086594546088No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTAACCCG10461.04292337604068No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGGAAGG10061.0030410289645546No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGACTGATT9650.9621616232115261No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGTAAGACG6920.6899646044169699No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGAATCTCTTT6190.6171793210030411No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA5770.5753028565731093No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGAATTGA5670.5653322698040779No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGATCACT5620.5603469764195623No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTT5560.5543646243581435No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGAGGGCCC5510.5493793309736278No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGTTAAGGCG5290.5274440400817588No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGATGGCCC5280.5264469814048557No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCCACC5220.5204646293434368No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA5050.5035146318360836No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGTGAATCT4910.4895558103594396No Hit
GATAAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGAAGAGAGCT4910.4895558103594396No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTTATG4880.48656463432873026No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGATGAAACCG4260.4247469963607358No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTTATA4070.4058028814995763No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATTACT4060.40480582282267313No Hit
ATTGAACGCTGGCGGCAGGCCTAATACATGCAAGTCGAACGGTAACAGAG3980.396829353407448No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGGTGGCCC3930.3918440600229323No Hit
GATGAACGCTGGCGGCGTGCTTAATACATGCAAGTCGAACGAACATTAAG3880.3868587666384167No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGATTCTCTTC3860.3848646492846104No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC3840.3828705319308041No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAATGAAGTT3810.3798793559000947No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAGGAGAA3780.37688817986938533No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGACATCT3680.366917593100354No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT3610.359938182362032No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG3440.3429881848546787No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAAG3430.34199112617777555No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGCGG3390.338002891470163No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATGCCG3310.33002642205493793No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC3260.32504112867042223No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGT3230.32204995263971287No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCCCTC3140.3130764245475846No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAT3060.30509995513235955No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA2980.29712348571713443No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT2870.2861558402712No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGAAGCTTTCT2870.2861558402712No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA2750.2741911361483623No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA2650.2642205493793309No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGGAATGTC2620.26122937334862156No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCCCCC2580.257241138641009No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAC2550.2542499626102996No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGCGCGGTTAAAGCC2540.2532529039333965No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGTTAAGGCG2510.2502617279026871No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT2400.2392940824567526No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGGAATGAC2400.2392940824567526No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGATTTACTTC2370.23630290642604315No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTTCTT2330.23231467171843062No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG2310.23032055436462434No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCTGC2310.23032055436462434No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGGAAGAGG2220.22134702627249614No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACCTT2220.22134702627249614No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAACC2120.2113764395034648No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGCTTT2040.2033999700882397No Hit
AGTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGCGAAAGTGG1990.198414676703724No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGGGTGCGTT1970.19642055934991776No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAAGAA1960.1954235006730146No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATCG1950.19442644199611148No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTACAT1720.17149409242733935No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAAA1700.1694999750735331No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGTACGCGAAAGGGA1700.1694999750735331No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTTTCA1640.16351762301211425No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTGTTT1620.16152350565830798No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATATT1600.15952938830450172No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGGGTTATAC1350.1346029213819233No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAGGACAA1350.1346029213819233No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAACC1320.13161174535121392No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGTACGCGAAGGCCT1300.12961762799740764No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGCGAAAGTGG1280.1276235106436014No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGGACTGTTC1270.12662645196669825No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTGAAGCT1270.12662645196669825No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAATC1240.12363527593598883No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGCGAAAGTGG1240.12363527593598883No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGAACAGG1210.12064409990527943No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAACCACTTC1190.11864998255147316No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGATCATT1170.1166558651976669No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGTTAAAGCA1170.1166558651976669No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTT1150.11466174784386061No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTATTT1130.11266763049005435No Hit
GATAAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGAGAGAGGAC1110.11067351313624807No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAAGTGGTCC1100.10967645445934494No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGCTTT1080.10768233710553866No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGCTTA1080.10768233710553866No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGATCCCAG1080.10768233710553866No Hit
GATGAACGCTGGCGGCGTGCCTAAGACATGCAAGTCGAACGAATCTTCTT1050.10469116107482926No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTCAAGA1040.10369410239792613No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTTTACT1040.10369410239792613No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACATC1010.10070292636721673No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAACG108.428883E-4295.287572
GACAAAC108.428883E-4295.287571
CACGCTG108.428883E-4295.287575
ACGCTGG77900.0295.287576
ACGCTAG22150.0295.287576
TGAACGC60400.0295.043123
GCTGGCT108.4541267E-4294.9938
GATGCAA108.4541267E-4294.993295
ATTGGTT108.4541267E-4294.993295
CTGGCTA108.4541267E-4294.9939
CTAGCTA19650.0294.9939
CGCTAGC22200.0294.622537
CGCTGGC78150.0294.342967
AACGCTG78100.0294.153325
GCTGGCG78150.0293.86068
AAACGCT4000.0291.596474
TAAACGC3900.0291.50183
ATAAACG3900.0291.50182
GAACGCT97550.0290.44434
GACGAAC36150.0290.386541
Produced by FastQC (version 0.11.5)