FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100295
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT1089210.859963108828955No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGTTCATCCTCTCAGACCGGCT83288.303504661249313No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT67576.737125479834488No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGGGACCTTCCTCTCAGAACCCCT58335.81584326237599No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGTTCATCCTCTCAGACCGGCT46624.648287551722419No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT46224.608405204646293No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT35873.5764494740515476No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT31953.185602472705519No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT26852.6771025474849197No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT20572.05094969838975No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGTCGGCT18391.8335909068248668No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT17971.7917144423949352No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTCCGCCCTCTCAGGCCGGCT17441.7388703325190689No Hit
TTGGACCGTGTTTCAGTTCCAATGTGGCCGATCATCCTCTTAAATCGGCT13251.3211027468966547No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGCCGGCT11231.1196968941622214No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTCCGCCCTCTCAGGCCGGCT10381.0349469066254549No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT10201.0169998504411983No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGTCGGCT9940.991076324841717No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAGCCTCTCAGCCCGGCT9400.9372351562889476No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT8040.8016351762301211No Hit
CTGGGCCGTGTCTCAGTCCCAATCTGGCCGGTCGGTCTCTCAACCCGGCT7980.7956528241687024No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT7350.7328381275238047No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTCCGCCCTCTCAGGCCGGCT7280.7258587167854829No Hit
TTGGACCGTGTTTCAGTTCNAATGTGGCCGATCATCCTCTTAAATCGGCT7130.7109028366319358No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT6350.6331322598334912No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGCCGGCT6280.6261528490951692No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT6190.6171793210030411No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGATCGGTCTCTCAACCCGGCT6100.6082057929109128No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCGGTCTCTCAACCCGGCT6060.6042175582033003No Hit
CTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT6010.5992322648187846No Hit
ATGGGCCGTGTCTCAGTCCCATTGTGGGGGGCCTTCCTCTCAGAACCCCT5620.5603469764195623No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAGCCTCTCAGCCCGGCT5090.5075028665436961No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT4960.4945411037439553No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT4650.4636322847599581No Hit
CTGGGCCGTGTCTCAGTCCNAATCTGGCCGGTCGGTCTCTCAACCCGGCT4450.44369111122189536No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGGGACCTTCCTCTCAGATCCCCT4330.43172640709905774No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCACCCTCTCAGGTCGGCT4290.4277381723914452No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCGGCCTCTCAGCCCGGCT4060.40480582282267313No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGTTCATCCTCTCAGATCGGCT4050.40380876414576994No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCACCCTCTCAGGTCGGCT4030.4018146467919637No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGGTCAACCTCTCAGTCCGGCT4020.4008175881150606No Hit
CTGGACCGTCTCTCAGTTCNAATGTGGCCGATCACCCTCTCAGGTCGGCT3880.3868587666384167No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAGCCTCTCAGCCCGGCT3840.3828705319308041No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTCCGCCCTCTCAGGCCGGCT3840.3828705319308041No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGGTCGGTCTCTCAACCCGGCT3550.3539558303006132No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGCCGATCACCCTCTCAGGTCGGCT3450.34398524353158183No Hit
TTGGACCGTATCTCAATTCCAATGTGGCCGATCGACCTCTCAGTCCGGCT3450.34398524353158183No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGATCGGTCTCTCAACCCGGCT3200.31905877660900345No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCTGATCATCCTCTCAGACCAGCT3180.31706465925519717No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT3020.301111720424747No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCAACCTCTCAGTTCGGCT2890.2881499576250062No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTTCGGCT2870.2861558402712No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCTGTTCATCCTCTCAGACCAGCT2860.2851587815942968No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGGGGGTCTTCCTCTCAGAACCCCT2860.2851587815942968No Hit
ATGGGCCGTGTCTCAGTCCNATTGTGGGGGGCCTTCCTCTCAGAACCCCT2830.28216760556358744No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT2730.2721970187945561No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCAACCTCTCAGTCCGGCT2630.2622264320255247No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCGGCCTCTCAGCCCGGCT2400.2392940824567526No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT2260.2253352609801087No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTTCGGCT2210.220349967595593No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCAACCTCTCAGTCCGGCT2170.21636173288798044No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCAGCCTCTCAGCCCGGCT2140.21337055685727108No Hit
TTGGACCGTATCTCAATTCNAATGTGGCCGATCGACCTCTCAGTCCGGCT1920.19143526596540206No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT1890.18844408993469267No Hit
CTGGGCCGTGTCTCAGTCCCAATGCGGCCGACCGGCCTCTCAGCCCGGCT1830.18246173787327385No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGGGGGTCTTCCTCTCAGAACCCCT1770.17647938581185504No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGGGGCCTTCCTCTCAGAACCCCT1690.16850291639662995No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT1680.16750585771972681No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCCGATCACCCTCTCAGGTCGGCT1650.1645146816890174No Hit
CTGGGCCGTCTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT1580.15753527095069544No Hit
CTGGACCGTGTTTCAGTTCCAGTGTGGCCGATCATCCTCTCAGATCGGCT1580.15753527095069544No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGTTCATCCTCTCAGACCGGCT1450.14457350815095468No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCGACCTCTCAGTCCGGCT1440.14357644947405154No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCACCCTCTCAGGTCGGCT1420.14158233212024526No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGCGACCTTCCTCTCAGAACCCCT1410.14058527344334215No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCTGATCATCCTCTCAGACCAGCT1380.13759409741263273No Hit
CTGGGCCGTGTCTCAGTCCCAATCTGGCTGGTCGGTCTCTCAACCCAGCT1370.1365970387357296No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCATCCTCTCAGACCGGCT1320.13161174535121392No Hit
CTGGACCGTGTCTCAGTTCCAATGTGGCCGTTCATCCTCTCAGACCGGCT1250.12463233461289197No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGCCGGCT1250.12463233461289197No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCTGGTCATCCTCTCAGACCAGCT1210.12064409990527943No Hit
TTGGTCCGTGTCTCAGTACNAATGTGGGGGACCTTCCTCTCAGAACCCCT1200.1196470412283763No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCTGTTCATCCTCTCAGACCAGCT1160.11565880652076375No Hit
TTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT1150.11466174784386061No Hit
TTGGACCGTGTGTCAGTTCCAATGTGGCCGATCATCCTCTGAAATCGGCT1120.11167057181315121No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT1080.10768233710553866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCT13500.0292.36067
GACCGTA1050.0292.36064
AGGCATT350.0292.3606295
GGACCGT42500.0292.016663
CTGGGCC43250.0291.684631
CGTGTCT81600.0291.6447
TGGACCG42600.0291.331182
GTATCTC13500.0291.277778
CCGTGTC82050.0290.935336
TGTCTCA81750.0290.930089
GACCGTG40050.0290.53564
ACCGTGT40000.0290.533365
GCCGTGT44600.0290.394045
CCGTATC13600.0290.210886
GGCCGTG44800.0288.771364
CTGGACC18150.0288.333621
TTGGACC24850.0288.242861
GCCGTAT12650.0287.73835
GGCCGTA12800.0285.50844
GTGTCTC83600.0284.492038
Produced by FastQC (version 0.11.5)