FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150473
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG2038313.545951765433001No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT143929.564506589221986No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA124898.299827876097373No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT82235.4647677656456635No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA72604.8247858419782945No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGA41922.785881852558266No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA41452.754647013085404No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGACGG39452.621732802562586No Hit
ATTGAACGCTGGCGGAACGCTTTACACATGCAAGTCGAACGGTAACGTGG36122.400430642042094No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC32562.1638433473114778No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTG27141.8036458367946409No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC24061.5989579525895012No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGTT23861.5856665315372194No Hit
GACGAACGCTGGCGGCACGCCTAACACATGCAAGTCGAACGGACTTAAAA22781.5138928578548976No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGATTT19771.3138569710180565No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA15010.9975211499737494No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTGAAA14730.9789131605005549No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGAATCTTT13890.9230891920809713No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGAGGG13310.8845440710293541No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA11490.7635921394535896No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAACACCTT11040.7336864420859556No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTCT10180.6765333315611439No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTA10120.6725459052454593No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACGAGGAG9780.6499504894565802No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA9650.6413110657725971No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGTTT8950.5947910920896108No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG8700.5781768157742585No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAATTT8690.5775122447216444No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAAGTTTTTA8260.5489356894592385No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA7810.5190299920916045No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGTGAGAGA7610.5057385710393227No Hit
ATTGAACGCTGGCGGAACGCTTTACACATGCAAGTCGAACGGTAACGCGG7380.49045343682919856No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCAGTTT7030.46719344998770546No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCGGGA6970.4632060236720209No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGCAAATAC6810.4525728868301954No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGATTT6570.4366231815674573No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCATTTT6570.4366231815674573No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCGCTTC6550.4352940394622291No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA6340.42133804735733316No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTCGTTT6010.39940720262106827No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGGAGATAAAT5670.37681178683218913No Hit
AACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGGGAGAATTG5290.3515580868328537No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCGGTTT5050.3356083815701156No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT5050.3356083815701156No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGTTTTTG4730.3143421078864647No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGGGTTTGG4710.3130129657812365No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGAGCGAT4310.28643012367667287No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTT4280.2844364105188306No Hit
GATGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA4260.28310726841360245No Hit
AGTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA4150.27579698683484744No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAAATC4150.27579698683484744No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG4110.27313870262439105No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGGTTT3920.2605118526247234No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGG3820.25386614209858244No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTATTT3810.2532015710459684No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA3760.24987871578289791No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGCTTA3720.24722043157244158No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTA3490.2319352973623175No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT3410.22661872894140478No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGTGCTCA3340.22196673157310615No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTCAAGA3260.2166501631521934No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATACT3200.21266273683650888No Hit
GACGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA2980.19804217367899887No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACATT2980.19804217367899887No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCAT2840.18873817894240164No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT2830.18807360788978755No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC2820.18740903683717344No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATATT2780.18475075262671709No Hit
AGTGAACGCTGGCGGAACGCTTGACACATGCAAGTCGAACGGTAACGTGG2640.17544675789011982No Hit
GATAAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGTTTTTC2500.16614276315352255No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATTACT2340.1555096263116971No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCGATTT2250.1495284868381703No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGGTTT2250.1495284868381703No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCACGA2210.14687020262771394No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCCCA2190.14554106052248578No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTTGA2140.1422182052594153No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG2110.14022449210157303No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCGGAT2080.1382307789437308No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTAAAT2040.1355724947332744No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGAG2010.13357878157543215No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA2000.13291421052281804No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAAGTGAAGA1950.1295913552597476No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGACGG1950.1295913552597476No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCAAT1940.12892678420713352No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCAATTT1710.11364164999700943No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAGTCAT1670.11098336578655307No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCGATTA1630.1083250815760967No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACCTT1610.10699593947086854No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAAGTCAAGA1560.10367308420779807No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCTCA1540.1023439421025699No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAACGC115600.0295.383363
CGCTAGC57550.0295.383367
ACGCTAG57650.0295.12726
CGCTGGC92500.0295.064037
AGATACG255.293259E-10294.9907295
GCTAGCG4100.0294.99078
CTAGCGG3600.0294.99079
CTAGCTA53400.0294.714489
CTGGCGG92600.0294.512859
AACGCTG92750.0294.26875
ACGCTGG92900.0293.793586
GCTAGCT54050.0291.443188
ATTGAAC10650.0291.223021
ATGAACG105750.0290.63492
ACGAACG34200.0290.633062
GATGAAC105550.0290.62591
GACGAAC33850.0289.2751
AACGCTA60500.0281.22455
AAACGCT750.0275.691134
GCTGGCG99950.0272.707768
Produced by FastQC (version 0.11.5)