FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005103565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150473
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT2879019.13300060475966No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGGGACCTTCCTCTCAGAACCCCT1606610.676998531297974No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT133388.864048699766736No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT115117.649877386640792No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGTCGGCT74594.957035481448499No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT62734.168854213048188No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT40002.658284210456361No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGTTCATCCTCTCAGACCGGCT34102.266187289414048No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCT33422.2209964578362893No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT33162.203717610468323No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCTGGTCATCCTCTCAGACCAGCT22141.4713603104875956No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCACCCTCTCAGGTCGGCT22131.4706957394349816No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGTCGGCT18991.2620204289141572No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGTTCATCCTCTCAGACCGGCT18711.2434124394409627No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCTGGTCGTCCTCTCAGACCAGCT18601.2361021578622078No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT15861.054009689445947No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT13750.9137851973443741No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT12930.8592903710300187No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGGGACCTTCCTCTCAGATCCCCT12470.8287201026097705No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCCGGTCACCCTCTCAGGTCGGCT12190.8101121131365759No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT11220.7456487210330093No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT9910.6585899131405634No Hit
CTGGGCCGTGTCTCAGTCCCAATGCGGCCGATCAACCTCTCAGTTCGGCT9020.5994430894579095No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGGTCAACCTCTCAGTCCGGCT8970.596120234194839No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT8870.589474523668698No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT8340.5542522578801512No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT7860.5223528473546749No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCAACCTCTCAGTCCGGCT7760.5157071368285341No Hit
CTGGTCCGTGTCTCAGTACNAGTGTGGGGGGTTAACCTCTCAGTCCCCCT7390.4911180078818127No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT6320.42000890525210505No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGTCGGCT6300.4186797631468769No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGCCGGCT6240.4146923368311923No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCACCCTCTCAGGTCGGCT5750.38212835525310185No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGCCGGCT5570.37016607630604825No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGCCGGCT5170.3435832342014846No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT5100.338931236833186No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT5010.33295009735965925No Hit
CTGGGCCGTGTCTCAGTCCNAATGCGGCCGATCAACCTCTCAGTTCGGCT4900.32563981578090423No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGGTTAACCTCTCAGTCCCCCT4520.3003861157815688No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT4490.2983924026237265No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTTCGGCT4480.29772783157111243No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGCCGATCACCCTCTCAGGTCGGCT4170.2771261289400756No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGTCGGCT4090.27180956051916294No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGGACATCCTCTCAGACCGGCT3900.2591827105194952No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACTCTCTCAAGCCGGCT3510.2332644394675457No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT3180.2113335947312807No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT3090.2053524552577539No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGCGACCTTCCTCTCAGAACCCCT2840.18873817894240164No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCAACCTCTCAGTCCGGCT2760.1834216105214889No Hit
TTGGTCCGTGTCTCAGTACNAATGTGGGGGGTTAACCTCTCAGTCCCCCT2340.1555096263116971No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGCCAACCTCTCAGTCCGGCT2320.15418048420646893No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACTCTCTCAAGTCGGCT2260.1501930578907844No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGGACATCCTCTCAGACCGGCT2060.1369016368385026No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGAAAACCTCTCAGTCCCCCT1910.12693307104929122No Hit
TTGGACCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT1790.11895821841792216No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCTGGTCATCCTCTCAGATCAGCT1750.11629993420746579No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCGGACCTTCCTCTCAGAACCCCT1750.11629993420746579No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCATCCTCTCAGACCGGCT1740.11563536315485169No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGCGACCTTCCTCTCAGAACCCCT1590.10566679736564034No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)