FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005103568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185384
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGAATTTT2080611.223190782376042No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAC120636.507034048245804No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT89934.851011953566651No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGA73363.957191559142105No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA64983.5051568635912487No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTGTTT63163.4069822638415395No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGTTT53062.862167177318431No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGATTACA39322.121002891295905No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT37822.040089759634057No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGATGGCCC33481.8059810986924436No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG29661.5999223233936049No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCGATTT26421.4251499590040133No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT26251.4159798040823373No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG25181.3582617701635524No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCTGC24261.3086350494109524No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATATTT23441.264402537435809No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGGAAGAGG22441.2104604496612437No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTAACCCG18791.0135718292840805No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTCAAGA17660.9526172700988218No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGGTGGCCC17080.921330859189574No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGACGCAT16440.8868079230138524No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT15270.8236956803176111No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA15170.8183014715401544No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGATAGAT15050.8118284210072068No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGATGAAACCG13290.7168903465239719No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA11310.6100850127303328No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAATCAAGA11280.6084667500970957No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGTTT10960.591205282009235No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCCCTC10890.5874293358650153No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCTTGAT10740.5793380226988306No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC10620.5728649721658827No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGAATCTTT10320.5566823458335132No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC9890.5334872480904501No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACCCCTT9720.5243170931687741No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAACC9360.5048979415699305No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGACTTGT8960.48332110646010445No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTAAAT8910.48062400207137623No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGCAAATAC8820.4757692141716653No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTTC8810.4752297932939196No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATACTG8400.4531135373063479No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTATGT8160.44016743624045224No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTGCTTGA7860.4239848099080827No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGC7440.4013291330427653No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTGAAA7170.3867647693436327No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGCTTATTT6900.3722004056445001No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGCGAAAGGGG6820.36788503862253485No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGGAATGTC6480.3495447287791827No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTA6470.349005307901437No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGA6380.34415052000172613No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAACACT6150.33174383981357614No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA6110.3295861563025936No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAT5820.31394295084796964No Hit
GACGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT5540.29883916627109136No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA5350.288590169593924No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAACACCTT5010.2702498597505718No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG4980.2686315971173348No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG4840.2610797048288957No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA4800.2589220213179131No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCACTTT4770.25730375868467614No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAACCTT4620.24921244551849134No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGAATTTT4620.24921244551849134No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTTGTAAA4590.2475941828852544No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGG4510.2432788158632892No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCTATC4100.22116255987571742No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC4010.21630777197600656No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGCTGTTA3870.20875587968756742No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCC3860.20821645880982179No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGATGATA3810.20551935442109354No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGGAATGAC3760.20282225003236523No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGAACGGG3410.18394251931126743No Hit
GATGAACGCTGGCGGCGTGCTTAACACATTCAAGTCGAACGGAGTTCAAT3400.18340309843352176No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTATTT3370.18178483580028482No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA3210.17315410175635437No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAC3190.17207526000086307No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATAGAC3150.16991757648988048No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAATTTCA3060.1650627885901696No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTCGTTT2980.16074742156820437No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGTAG2940.15858973805722176No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACATA2930.15805031717947612No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGAGGG2920.15751089630173046No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTAAGC2890.1558926336684935No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGGGGTTACGG2860.15427437103525654No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGCGAAAGGGA2770.14941958313554568No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGGTTT2740.14780132050230874No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTG2720.14672247874681743No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTATGA2690.14510421611358046No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGACGG2690.14510421611358046No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAATC2680.1445647952358348No Hit
GATTAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGTAGCA2650.14294653260259785No Hit
GATGAACGCTGGCGGCGTGCTTAATACATGCAAGTCGAACGAAGCACCTT2630.14186769084710654No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGAATAAG2490.1343157985586674No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT2490.1343157985586674No Hit
GATTAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGGAACACCCC2490.1343157985586674No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGACGGACTT2460.13269753592543046No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGGGGATATTG2410.1300004315367022No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAACGCCAA2290.12352738100375436No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGGACCCT2240.12083027661502611No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTGCTTA2220.11975143485953481No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGATTT2190.11813317222629785No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGG2160.11651490959306088No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAGAAGAT2140.11543606783756957No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAAGTGGCCC2120.11435722608207828No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGCTTAATT2110.11381780520433263No Hit
GATGAACGCTGGCGGCGTGCATAACACATTCAAGTCGAACGGACGACTCG2050.11058127993785871No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGACGAAGCC1990.1073447546713848No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTAACCCT1910.10302938764941959No Hit
GACGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGATGGCCC1910.10302938764941959No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGACATTG1900.10248996677167392No Hit
GACGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA1880.10141112501618262No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGGGGCAGCATGA1870.10087170413843698No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGGTTGCCC1860.10033228326069132No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)