FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143731
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT1844812.835087768122394No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT1702911.847826843200144No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT133369.278443759522997No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCTGGTCATCCTCTCAGACCAGCT101707.075717834009366No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGGGACCTTCCTCTCAGAACCCCT92936.465550229247692No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT73845.137374679088018No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT38422.6730489595146487No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT36712.55407671274812No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT36122.5130278088930016No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT27561.9174708309272184No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT23831.6579582692668946No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTAAGACCGGCT23501.6349987128733539No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT22381.5570753699619428No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT21281.4805435153168072No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGTCGGCT20341.415143566801873No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGCCGGCT20001.3914882662751946No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCACCCTCTCAGGTCGGCT15091.0498778969046343No Hit
CTGGTCCGTGTCTCAGTACNAGTGTGGGGGGTTAACCTCTCAGTCCCCCT12610.8773333518865102No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGTCGGCT12520.8710716546882719No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCATCCTCTAAGACCGGCT12430.8648099574900335No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT10250.7131377364660373No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGTCCGATCAGCCTCTCAGCTCGGAT9830.6839164828742581No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT9410.654695229282479No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCTGGTCATCCTCTCAGATCAGCT8150.5670314685071418No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT7290.5071974730573084No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGGGACCTTCCTCTCAGATCCCCT7240.5037187523916205No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGCCAACCTCTCAGTCCGGCT6730.468235801601603No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGTCCGATCAGCCTCTCAGCTCGGAT6410.4459719893411999No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGGTCAACCTCTCAGTCCGGCT6080.42301243294765917No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT5570.3875294821576417No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTTCGGCT5470.3805720408262657No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT5460.3798762966931281No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT5330.3708316229623394No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCATCCTCTCAGACCGGCT5140.357612484432725No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCACCCTCTCAGGTCGGCT4390.3054316744474052No Hit
TTGGTCCGTGTCTCAGTACNAATGTGGGGGACCTTCCTCTCAGAACCCCT4360.30334444204799244No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGGACATCCTCTCAGACCGGCT4190.2915167917846533No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCAACCTCTCAGTCCGGCT4160.2894295593852405No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGCCAACCTCTCAGTCCGGCT3870.26925297952425015No Hit
TTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT3460.24072747006560866No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGCCGGCT3040.21150621647382958No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT2920.2031572868761784No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCGACCTCTCAGTCCGGCT2840.19759133381107766No Hit
CTGGACCGTGTTCCAGTTCCAGTGTGGCCGGTCATCCTCTCAGACCGGCT2780.19341686901225205No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGGTTAACCTCTCAGTCCCCCT2690.18715517181401367No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCGCCCTCTCAGGCCGGCT2510.17463177741753694No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGGACATCCTCTCAGACCGGCT2290.1593254064885098No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCCGGTCACCCTCTCAGGTCGGCT2220.1544551975565466No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT2150.14958498862458341No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGTCGGCT2120.14749775622517064No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCCGCT2020.14054031489379465No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT2010.13984457076065707No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGAAAACCTCTCAGTCCCCCT1990.13845308249438187No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCT1990.13845308249438187No Hit
CTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT1950.13567010596183146No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGCGACCTTCCTCTCAGAACCCCT1840.12801692049731792No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTGAACCTCTCAGTCCCCCT1840.12801692049731792No Hit
CTGGGCCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT1820.12662543223104272No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGCCGGCT1760.12245096743221714No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGGTCATCCTCTCAGACCAGCT1710.11897224676652915No Hit
CTGGACCGTGTTCCAGTTCNAGTGTGGCCGGTCATCCTCTCAGACCGGCT1590.11062331716887798No Hit
TTGGACCGTGTCTCAGTTCNAGTGTGGCTGGTCATCCTCTCAGACCAGCT1510.10505736410377718No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGGTCATCCTCTCAGACCAGCT1500.1043616199706396No Hit
CTGGGCCGTGTCTCAGTCCCAATGCGGCCGATCAACCTCTCAGTTCGGCT1490.103665875837502No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTTCGGCT1440.10018715517181402No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCT108.6870557E-4292.371987
GAGTCTC108.6870557E-4292.371988
GACCGTA350.0292.371984
GTGTCTC132100.0292.039958
CGTGTCT132000.0292.039737
TGTCTCA132150.0291.929479
TGGACCG64650.0291.919742
GGACCGT64600.0291.919373
GCCGTGT62400.0291.434885
GACCGTG64100.0291.003574
TTGGACC30800.0290.948061
CCGTGTC132900.0290.831976
CTGGGCC45350.0290.437841
CTGGACC34000.0289.79221
CTGGTCC5200.0289.560671
ACCGTGT64550.0289.427865
CGTATCT9850.0289.40377
GTATCTC9850.0289.40378
TTGGGCC26700.0287.991851
GCCGTAT9550.0286.248965
Produced by FastQC (version 0.11.5)