FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177340
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT2063811.637532423593099No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT119686.748618472989737No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT116936.5935491146949365No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT89525.047930528927484No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT78164.407353107026052No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT66673.7594451336415924No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGTCGGCT50582.8521484154731027No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT47092.6553513025826097No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT46712.6339235367091463No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCACCCTCTCAGGTCGGCT44962.535243035976091No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT37292.102740498477501No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCGCCCTCTCAGGCCGGCT35642.0096988835006204No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGTCCGATCAGCCTCTCAGCTCGGAT31381.7694823502875834No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGGGACCTTCCTCTCAGAACCCCT26511.494868613961881No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCAACCTCTCAGTCCGGCT26261.4807713995714447No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT22571.2726965151686027No Hit
TTGGACCGTATCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT20781.1717604601330776No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCTGGTCATCCTCTCAGACCAGCT20471.1542799142889364No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGTTCATCCTCTCAGACCGGCT20441.1525882485620842No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCGCCCTCTCAGGCCGGCT19911.122702154054359No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT19711.1114243825420096No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGTCCGATCAGCCTCTCAGCTCGGAT19671.10916882823954No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGGACATCCTCTCAGACCGGCT18701.0544716364046465No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT18421.0386827562873575No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCGGTCTCTCAACCCGGCT16180.912371715349047No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT14480.8165106574940791No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTTCGGCT12620.711627382429232No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGCCGGCT11980.6755385135897147No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGTTCATCCTCTCAGACCGGCT11880.6698996278335401No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT11560.6518551934137814No Hit
CTGGGCCGTGTCTCAGTCCCAATGCGGCCGATCAACCTCTCAGTTCGGCT11190.6309913161159355No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCCGATCACCCTCTCAGGTCGGCT11110.6264802075109958No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT10850.6118191045449419No Hit
TTGGACCGTATCTCAGTTCNAATGTGGCCGATCACCCTCTCAGGTCGGCT10830.610691327393707No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGTCGGCT9680.5458441411976993No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGGACATCCTCTCAGACCGGCT9650.544152475470847No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGGTCGGTCTCTCAACCCGGCT8430.4753580692455171No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGGTCAACCTCTCAGTCCGGCT8130.4584414119769934No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT7260.4093831058982745No Hit
CTGGTCCGTGTCTCAGTACNAGTGTGGGGGGTTAACCTCTCAGTCCCCCT7030.39641366865907296No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTCCATCCTCTCAGACCGGCT6650.37498590278560956No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT6500.3665275741513477No Hit
CTGGGCCGTGTCTCAGTCCNAATGCGGCCGATCAACCTCTCAGTTCGGCT6240.3518664711852938No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGCCGATCACCCTCTCAGGTCGGCT5800.3270553738581256No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT5630.3174692680726289No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTTCGGCT5580.31464982519454154No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT5410.3050637194090448No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCGCCCTCTCAGGCCGGCT5390.30393594225780984No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCAACCTCTCAGTTCGGCT5170.2915303935942258No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTTCGGCT5080.28645539641366863No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCAGCCTCTCAGCTCGGCT4830.27235818202323225No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT4410.24867486184729898No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTCCAACCTCTCAGTCCGGCT4030.22724709597383555No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGCCGGCT3910.22048043306642606No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCACCCTCTCAGGTCGGCT3830.21596932446148642No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGCCGGCT3640.20525544152475472No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT3540.19961655576858015No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT3510.19792489004172775No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGCCAACCTCTCAGTCCGGCT3500.1973610014661103No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTCCATCCTCTCAGACCGGCT3390.19115822713431824No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGATCAACCTCTCAGTTCGGCT3340.18833878425623096No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCACCCTCTCAGGCCGGCT3300.18608322995376111No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCACCCTCTCAGGTCGGCT3300.18608322995376111No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT3300.18608322995376111No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT3280.18495545280252623No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCTGATCATCCTCTCAGACCAGCT3090.17424156986579453No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGTCCGTTCAGCCTCTCAGCCCGGAT3010.16973046126085484No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT2990.16860268410961993No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT2830.1595804668997406No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGATCAGCCTCTCAGCTCGGCT2750.15506935829480095No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGAAAACCTCTCAGTCCCCCT2720.15337769256794856No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCGCCCTCTCAGGCCGGCT2670.15055824968986128No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCAACCTCTCAGTCCGGCT2500.1409721439043645No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGTTCATCCTCTCAGACCGGCT2480.13984436675312958No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTCCAACCTCTCAGTCCGGCT2470.13928047817751213No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGGTTAACCTCTCAGTCCCCCT2410.13589714672380737No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACTCTCTCAAGTCGGCT2410.13589714672380737No Hit
TTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT2310.1302582609676328No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCAACCTCTCAGTCCGGCT2120.1195443780309011No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCATCCTCTCAGACCGGCT2100.11841660087966617No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGATCGTCCTCTCAGACCAGCT2020.11390549227472652No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGGGACCTTCCTCTCAGATCCCCT2000.1127777151234916No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT1980.11164993797225667No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCTGATCATCCTCTCAGACCAGCT1950.1099582722454043No Hit
CTGGACCGTATCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT1910.10770271794293448No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCCGGTCACCCTCTCAGGTCGGCT1900.10713882936731702No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCGCCCTCTCAGGTCGGCT1840.10375549791361227No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGCCAACCTCTCAGTCCGGCT1820.10262772076237736No Hit
TTGGTCCGTGTCTCAGTACNAATGTGGGGGACCTTCCTCTCAGAACCCCT1790.10093605503552498No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCTA108.690999E-4292.3469295
GACCGTG26850.0291.802464
GTGTCTC126550.0291.769328
CGTGTCT126600.0291.538677
TGGACCG32450.0291.445952
CCGTATC49250.0291.15976
CTGGGCC105550.0291.10051
GCCGTGT96100.0290.977945
CGTATCT49100.0290.858377
TGTCTCA127150.0290.507489
GGCCGTG96350.0290.22294
GGACCGT32650.0290.108373
GGCCGTA44600.0290.052644
CCGTGTC127450.0289.93846
GCCGTAT44650.0289.727845
TTGGACC16250.0289.64831
ACCGTGT27200.0289.122475
GTATCTC49400.0289.091988
TTGGGCC35500.0286.170531
CTGGACC16750.0285.365451
Produced by FastQC (version 0.11.5)