FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164624
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT1935611.757702400622023No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT106346.459568471182816No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT95665.810817377782097No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGTTCATCCTCTCAGACCGGCT88595.381353873068325No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT59323.603362814656429No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGGGACCTTCCTCTCAGAACCCCT52233.1726844202546407No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGTTCATCCTCTCAGACCGGCT51063.101613373505686No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT44712.7158858975605016No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT44572.707381669744387No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT40262.4455729419768684No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT34722.1090484983963456No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT34602.1017591602682475No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCAACCTCTCAGTCCGGCT28871.7536932646515697No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGTCGGCT25921.574497035669161No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGGTCAACCTCTCAGTCCGGCT25461.5465545728447856No Hit
CTGGTCCGTGTCTCAGTACNAGTGTGGGGGGTTAACCTCTCAGTCCCCCT21081.2804937311692097No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT19261.169938769559724No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGCCGGCT18811.1426037515793566No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCCGATCAACCTCTCAGTCCGGCT16861.0241520069977645No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCT16821.021722227621732No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCAGCCTCTCAGCTCGGCT15760.9573330741568665No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGGACATCCTCTCAGACCGGCT14710.8935513655360093No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT14200.8625716784915929No Hit
CTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT13360.8115463115949071No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCACCCTCTCAGGTCGGCT12100.7350082612498785No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT11060.6718339974730294No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTCCAACCTCTCAGTCCGGCT11030.6700116629410049No Hit
CTGGACCGTGTTTCAGTTCCAGTGTGGCCGATCATCCTCTCAGATCGGCT10980.6669744387209641No Hit
TTGGTCCGTGTCTCAGTACNAATGTGGGGGACCTTCCTCTCAGAACCCCT10680.6487510934007192No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGGTTAACCTCTCAGTCCCCCT9880.600155505880066No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCTGGTCGTCCTCTCAGACCAGCT9730.5910438332199436No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGATCAGCCTCTCAGCTCGGCT9590.5825396054038293No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGTCGGCT8020.4871707648945476No Hit
CTGGACCGTCTCTCAGTTCNAATGTGGCCGATCACCCTCTCAGGTCGGCT7970.48413354067450676No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGGACATCCTCTCAGACCGGCT7710.4683399747302945No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTTCGGCT7630.4634804159782292No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT7420.45072407425405775No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCCGGTCACCCTCTCAGGTCGGCT7090.4306783944017883No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT6620.40212848673340457No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT6570.39909126251336374No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCGCCCTCTCAGGCCGGCT6560.39848381766935564No Hit
CTGGACCGTGTTTCAGTTCNAGTGTGGCCGATCATCCTCTCAGATCGGCT6510.39544659344931476No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT5840.3547477889007678No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCACCCTCTCAGGTCGGCT5710.34685100592866164No Hit
CTGGACCGTATCTCAGTTCCAATGTGGCCGTTCGACCTCTCAGTCCGGCT5560.3377393332685392No Hit
CTGGACCGTATCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT5560.3377393332685392No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTCCAACCTCTCAGTCCGGCT5460.3316648848284576No Hit
TTGGTCCGTGTCTCAGTACNAATGTGGGGGGTTAACCTCTCAGTCCCCCT5380.32680532607639223No Hit
CTGGGCCGTGTTTCAGTCCCAATGTGGCCGATCAGCCTCTCAGCTCGGCT5330.32376810185635146No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT5310.3225532121683351No Hit
TTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT5220.31708620857226166No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT5070.3079745359121392No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCAACCTCTCAGTTCGGCT5040.3061522013801147No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT4590.2788171833997473No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGCCGGCT4320.2624161726115269No Hit
CTGGGCCGTGTCTCAGTCCCAATCTGGCCGGTCGGTCTCTCAACCCGGCT4300.2612012829235105No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGTTCATCCTCTCAGATCGGCT4130.25087472057537175No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT4070.2472300515113228No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGAAAACCTCTCAGTCCCCCT4050.24601516182330643No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCGCCCTCTCAGGCCGGCT3990.24237049275925743No Hit
CTGGGCCGTGTCTCAGTCCCAATGCGGCCGATCAACCTCTCAGTTCGGCT3970.24115560307124115No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTCCACCCTCTCAGGCCGGCT3880.23568859947516763No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTGAACCTCTCAGTCCCCCT3820.23204393041111868No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGGGACCTTCCTCTCAGATCCCCT3710.22536203712702887No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT3540.2150354747788901No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGCCGATCACCCTCTCAGGTCGGCT3490.21199825055884924No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCAACCTCTCAGTCCGGCT3480.2113908057148411No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCCGATCACCCTCTCAGGTCGGCT3410.20713869180678393No Hit
CTGGGCCGTGTTTCAGTCCNAATGTGGCCGATCAGCCTCTCAGCTCGGCT3050.18527067742249004No Hit
CTGGACCGTATCTCAGTTCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT3030.1840557877344737No Hit
TTGGACCGTCTCTCAGTTCNAATGTGGCCGATCACCCTCTCAGGTCGGCT3000.1822334532024492No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACTCTCTCAAGCCGGCT2970.18041111867042472No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGACCGTCCACCCTCTCAGGCCGGCT2930.17798133929439208No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTTCGGCT2850.17312178054232674No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCGCCCTCTCAGGCCGGCT2830.17190689085431043No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGCCAACCTCTCAGTCCGGCT2680.16279521819418796No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT2640.1603654388181553No Hit
CTGGACCGTATCTCAGTTCNAATGTGGCCGTTCGACCTCTCAGTCCGGCT2630.15975799397414714No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGATCACCCTCTCAGGTCGGCT2470.1500388764700165No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCATCCTCTCAGACCGGCT2340.1421420934979104No Hit
CTGGGCCGTGTCTCAGTCCNAATCTGGCCGGTCGGTCTCTCAACCCGGCT2330.14153464865390222No Hit
CTGGGCCGTGTCTCAGTCCNAATGCGGCCGATCAACCTCTCAGTTCGGCT2300.13971231412187773No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGACCTTCCTCTCAGAACCCCT2280.1384974244338614No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGATCAGTCTCTCAACCCGGCT2260.13728253474584506No Hit
CTGGTCCGTGTCTCAGTACNAGTGTGGGGGGTGAACCTCTCAGTCCCCCT2260.13728253474584506No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTACATCCTCTCAGATCGGCT2100.12756341724171444No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTCCACCCTCTCAGGCCGGCT2070.12574108270968998No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGGTCAACCTCTCAGTTCGGCT2030.1233113033336573No Hit
CTGGTCCGTGTCTCAGTACNAGTGTGGGGGGAAAACCTCTCAGTCCCCCT2020.12270385848964914No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCAGCT1980.1202740791136165No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT1970.11966663426960832No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGACCGTCCACCCTCTCAGGCCGGCT1880.11419963067353485No Hit
TTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT1860.11298474098551851No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT1860.11298474098551851No Hit
CTGGACCGTTTCTCAGTTCCAATGTGGCCGGTCATCCTCTCAGATCGGCT1750.10630284770142871No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCTGGTCATCCTCTCAGACCAGCT1690.10265817863737972No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGTTCATCCTCTCAGACCGGCT1680.10205073379337157No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATTGT108.68954E-4292.35693295
GGTCTGG108.68954E-4292.35693295
GGCTGGA108.68954E-4292.35693295
ATATTTT108.68954E-4292.35693295
CGTGTCT108850.0292.088357
GGACCGT49250.0292.060123
GACCGTG43400.0292.02014
GTGTCTC108850.0291.954048
CTGGGCC87150.0291.6861
TTGGACC18950.0291.585541
CGTATCT48200.0291.447087
ACCGTGT43550.0291.349985
TGGACCG49400.0291.17332
TGTCTCA109050.0291.15059
GCCGTAT46850.0290.796845
CCGTGTC109600.0290.75646
CCGTATC48400.0290.54486
GTATCTC48400.0290.54488
GCCGTGT55000.0290.49655
GGCCGTA47150.0289.566654
Produced by FastQC (version 0.11.5)