FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163021
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA2068712.689776163807117No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT130257.9897681893743755No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATCA87295.354524877163065No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAT79504.876672330558639No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGTT70944.351586605406665No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA59013.619779046871262No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA36572.2432692720569745No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTATTT36452.2359082572183953No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGTGCTCA33632.062924408511787No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT33482.053723139963563No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCACTTT29631.8175572472258175No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTACAT29571.8138767398065279No Hit
GATGAACGCTAGCAGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGT28361.7396531735175222No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTGCTTGA27921.7126627857760657No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGTTT27811.7059151888407016No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGT27291.6740174578735254No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG23001.4108611773943234No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG20941.2844970893320493No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAATTTACA20301.2452383435262941No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCGAT19281.182669717398372No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT17921.0992448825611425No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGAGA15860.9728807944988682No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGTTACAC15080.9250341980481043No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGCTAGGA14350.8802546911134148No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA14090.8643058256298267No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTT13120.8048042890179793No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATATT12360.7581845283736451No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG12160.7459161703093466No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCGATTT11240.6894817232135737No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATTACT10950.6716926040203409No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGAATTTT10630.6520632311174634No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCATTTT10580.6489961416013889No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTTTAT10550.647155887891744No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAAA10380.6367277835370903No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAAAATTGA9720.5962422019249054No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA9210.5649578888609442No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGGAGATATTT9120.55943712773201No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACCTT8920.5471687696677116No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA7940.48705381515264906No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCGTTTT7480.45883659160476253No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAATCTT7460.45760975579833274No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTT7300.447795069346894No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTGCTT6240.3827727716061121No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGTTT6190.37970568209003747No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCGAGCACTT5640.3459676974132167No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAATTTT5580.34228718999392715No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAT5560.3410603541874973No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT5250.32204439918783473No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCGATTA5210.319590727574975No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCAATTA4880.29934793676888255No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATAAGC4850.29750768305923775No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGGTTT4760.29198692193030346No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAAGTGAAGA4760.29198692193030346No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA4020.24659399709239913No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGGACCCT4000.24536716128596928No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAGTTA3790.2324853853184559No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGATGATA3720.22819145999595142No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCATTTT3700.22696462418952157No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCCTT3580.21960360935094253No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGTTTTAT3540.21714993773808283No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCACGT3540.21714993773808283No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCAATTG3510.21530968402843803No Hit
AGTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA3490.2140828482220082No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGAATCTTT3460.21224259451236346No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACGAGAGG3450.2116291766091485No Hit
GATGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA3340.20488157967378434No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACCTT3200.19629372902877545No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA3090.18954613209341128No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGCTTA3070.18831929628698144No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAATATA2880.1766643561258979No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGGACGCAATGCTTC2720.16684966967445913No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCAATCA2720.16684966967445913No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATACTT2630.1613289085455248No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGATTATT2580.1582618190294502No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATTC2490.15274105790051587No Hit
GACGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA2430.14906055048122635No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGG1950.11961649112691003No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCGATTT1930.1183896553204802No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCGAGCAGCA1860.11409572999797571No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG1850.11348231209476078No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGATGAAACCG1840.11286889419154587No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG1780.10918838677225634No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCAGCTT1750.10734813306261157No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTACAT1720.10550787935296678No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGAGAATT1690.10366762564332202No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTTGAG1690.10366762564332202No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAGCTGAATT1680.1030542077401071No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACGC255.2386895E-10295.44243
TGAACAC108.4204884E-4295.44243
ATAAACG108.4204884E-4295.44242
GAACACT108.4204884E-4295.44244
CGCTGGC97600.0295.442387
AAACGCT350.0295.442384
TCCTGAA108.459298E-4294.98926295
CTGAGCA108.459298E-4294.98926295
AGAACTG206.16983E-8294.98926295
GAGACCC108.459298E-4294.98926295
GACTCAG108.459298E-4294.98926295
TGAACGC119550.0294.948153
ACGCTGG97950.0294.537546
CTGGCGG97850.0294.537059
CGCTAGC65000.0294.533367
ACGCTAG65000.0294.533366
CTAGCTA54350.0294.175149
GCTAGCT54450.0293.634868
ATTGAAC6050.0293.000731
AACGCTG99000.0291.26445
Produced by FastQC (version 0.11.5)