FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138464
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGCGGGGCAGCATGA130559.428443494337877No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA92586.68621446729836No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA54003.8999306678992376No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGCTTGTT52283.775710654032817No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT49613.5828807487866885No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAGCACAG48903.5316038825976426No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAATC38382.7718396117402357No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCTTGAT30142.17673908019413No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAATT27852.0113531314998845No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCGAA25211.8206898544025882No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGATCATG24961.8026346198289807No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGACGG22591.6314709960711808No Hit
GATGAACGCTAGCTATAGGCTTAACACATGCAAGTCGCGGGGCAGCATGA20381.4718627224404899No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGAGGG19211.3873642246360065No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG18171.312254448809799No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA18121.3086434018950774No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG17791.2848104922579155No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT15201.0977582620753408No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA12840.9273168477004854No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGAGAGAAGAG12670.9150392881904322No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC12210.8818176565749942No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA11590.8370406748324474No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACCAAATA11580.8363184654495032No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGTTAAAGCA10800.7799861335798475No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGC10660.7698752022186273No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCCCTC10500.7583198520915183No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTCAAGA10380.7496533394961867No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT9820.7092096140513058No Hit
AGTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA9720.7019875202218627No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGACGACCGA9240.6673214698405362No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGG8680.6268777443956551No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGATGGA8000.5777675063554426No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCGGGA7930.5727120406748325No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA7560.5459902935058932No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGAGCGTA7530.5438236653570603No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAACGCCAA7510.5423792465911718No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTCTTA7360.5315461058470071No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACCGAATT7260.5243240120175642No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAGTTA6800.49110238040212617No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACCTT6750.4874913334874047No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATATTG6390.4614917957014098No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATAAGC6140.4434365611278022No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGTTAAGGCG5840.42177027963947306No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA5780.4174370233418072No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT5710.4123815576611971No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAACACCAA5700.41165934827825285No Hit
AGTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAGCACAG5500.39721516061936674No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGACACGG5150.37193783221631616No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGTGAAGAGG5010.3618269008550959No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACAGAAGT4960.3582158539403744No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAGAAGAT4940.3567714351744858No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGAATTTT4870.3517159694938756No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGTACGAAGGAGCCC4420.31921654726138204No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCCACC4380.3163277097296048No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGGTGGCCC4370.3156055003466605No Hit
GACGAACGCTGGCGGCGTGCTTAATACATGCAAGTCGAACGGAGTTAAGG4320.311994453431939No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAAG4270.30838340651721746No Hit
GACGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA4160.30043910330483015No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC4130.2982724751559972No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT4060.2932170094753871No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGAAATTCT3970.28671712502888835No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGAAGACCT3830.27660619366766814No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGTT3710.2679396810723365No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGTCATTG3640.26288421539172635No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGTAG3630.2621620060087821No Hit
GATGAACGCTGGCGGCGTGCTTAACACATTCAAGTCGAACGGAGTTCAAT3430.247717818349896No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA3280.23688467760573148No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTAACCCG3140.22677374624451124No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTTTAT2970.21449618673445806No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG2920.21088513981973653No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATTACT2850.20582967413912642No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT2730.19716316154379476No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG2680.19355211462907326No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGATGAAA2680.19355211462907326No Hit
GACGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAGCACAG2650.19138548648024034No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGTACGAAGTAGCCC2550.18416339265079734No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACGAGAGG2540.183441183267853No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGTACGAAGGGGCCC2460.17766350820429858No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGG2440.17621908943840997No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGTTTTAT2430.17549688005546568No Hit
GATGAACGCCGGCGGCGTGCCTAATACATGCAAGTCGAGCGGGGCCCGCA2360.17044141437485555No Hit
GATGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA2360.17044141437485555No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAACC2350.16971920499191126No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGATGGCCT2330.16827478622602263No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGTACGCGAAAGGGA2320.16755257684307834No Hit
GATGAACGCTAGCTAGAGGCTTAACACATGCAAGTCGCGGGGCAGCATGA2270.16394152992835684No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGTTT2230.16105269239657963No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCGATTC2220.1603304830136353No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGAGA2220.1603304830136353No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAATGTA2150.1552750173330252No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTCATCA2080.15021955165241507No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTT2030.14660850473769355No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACATGT2010.14516408597180494No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGATTATC1900.1372197827594176No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA1880.135775363993529No Hit
GACGAACGCTGGCGGCGCGCTTAACACATGCAAGTCGAACGGAGCTGAGA1870.1350531546105847No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTC1870.1350531546105847No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAAGTTAGA1760.12710885139819736No Hit
GATGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAGCGGAGTTTTGT1760.12710885139819736No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGATTT1720.12422001386642016No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGATCCCAG1720.12422001386642016No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGTGAAAGTCC1690.12205338571758724No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGGAGATATTC1680.12133117633464294No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGTCATCG1660.11988675756875433No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCGATTT1590.11483129188814421No Hit
GACAAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGATTGTG1570.11338687312225562No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGACAGGG1560.11266466373931129No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCCGA1560.11266466373931129No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCGATTT1520.1097758262075341No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAACACCTT1510.10905361682458979No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG1480.10688698867575687No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGACAGATAA1470.10616477929281257No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGACGGACTT1450.10472036052692398No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGAGCTAA1430.10327594176103537No Hit
GATGAACGCTGGCGGCGCATTTAAGACATGCAAGTCGAACGAAGATTTAC1430.10327594176103537No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTT1430.10327594176103537No Hit
GACGAACGCTGGCGGCACGCCTAACACATGCAAGTCGAACGGACGAGGAG1430.10327594176103537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACGC303.6379788E-12295.416663
GACAAAC303.6379788E-12295.416661
ATAAACG15850.0295.416632
CGCTGGC108450.0295.144267
TGAACGC77400.0295.034973
CAAACTC108.4578706E-4294.9899295
GTTACTG108.4578706E-4294.9899295
CTGCGCC108.4578706E-4294.9899295
CCGAGAA108.4578706E-4294.9899295
AGCGCCC108.4578706E-4294.9899295
ACGCTAG29150.0294.90996
ACGCTGG108550.0294.736276
AAACGCT16200.0294.504854
GATAAAC15900.0294.487641
CTGGCGG108450.0294.44599
GCTGGCG108700.0294.175758
TAAACGC15950.0293.564483
AACGCTG109050.0293.520325
CTAGCTA24850.0292.615729
CGCTAGC29450.0291.905767
Produced by FastQC (version 0.11.5)