FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005103591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138464
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT110537.982580309683383No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT107247.744973422694708No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGGGGACCTTCCTCTCAGAACCCCT90636.54538363762422No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT84386.09400277328403No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT61384.432921192512134No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCTGGTCATCCTCTCAGACCAGCT60184.346256066558817No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT50613.6551016870811184No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGGGGACCTTCCTCTCAGAACCCCT49193.5525479547030274No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT46273.341662814883291No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT30942.2345158308296744No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGGGACCTTCCTCTCAGAACCCCT27381.9774092905015022No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGTTCATCCTCTCAGACCGGCT26541.9167437023341807No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCAGCCTCTCAGCTCGGCT25151.8163565981049223No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT20661.4920845851629303No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT19091.3786977120406747No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT18961.369308990062399No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGCCAACCTCTCAGTCCGGCT17651.2746995608966951No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCAACCTCTCAGTCCGGCT17561.2681996764501964No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGATCAGCCTCTCAGCTCGGCT15361.1093136122024498No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGTTCATCCTCTCAGACCGGCT14721.0630922116940142No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGATCAACCTCTCAGTTCGGCT11710.8457071874277791No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGTCGGCT10830.7821527617286803No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCATCCTCTCAGACCGGCT10700.7727640397504044No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGCCAACCTCTCAGTCCGGCT10050.7258204298590247No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT9810.7084874046683615No Hit
CTGGGCCGTGTCTCAGTCCCAATCTGGCCGGTCGGTCTCTCAACCCGGCT8700.6283221631615438No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCAACCTCTCAGTTCGGCT8300.5994337878437717No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT8230.5943783221631616No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT8030.5799341345042756No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT7890.5698232031430552No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTCCACCCTCTCAGGCCGGCT7850.566934365611278No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT7160.5171019181881211No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGCCACCCTCTCAGGCCGGCT6770.48893575225329333No Hit
CTGGGCCGTGTCTCAGTCCCAATCTGGCTGGTCGGTCTCTCAACCCAGCT6190.4470476080425237No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT6140.4434365611278022No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCGCCCTCTCAGGCCGGCT6110.44126993297896927No Hit
TTGGTCCGTGTCTCAGTACNAATGTGGGGGACCTTCCTCTCAGAACCCCT5210.376271088513982No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT5110.36904899468453894No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTTCGGCT4910.3546048070256529No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT4760.3437716662814883No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCTGGTCATCCTCTCAGATCAGCT4740.34232724751559973No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGCCGGCT4520.32643864109082504No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTTCGGCT4500.3249942223249364No Hit
CTGGGCCGTGTCTCAGTCCNAATCTGGCCGGTCGGTCTCTCAACCCGGCT4340.31343887219782757No Hit
CTGGTCCGTGTCTCAGTACNAGTGTGGGGGGTTAACCTCTCAGTCCCCCT4330.3127166628148833No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT4320.311994453431939No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGTTCATCCTCTCAGACCGGCT4260.3076611971342732No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCACCCTCTCAGGTCGGCT4250.3069389877513289No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGGTCAACCTCTCAGTCCGGCT4190.30260573145366304No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCCGATCAACCTCTCAGTTCGGCT4070.2939392188583314No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCCGATCACCCTCTCAGGTCGGCT4010.28960596256066556No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAGTCTCTCAACTCGGCT3940.28455049688005546No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTCCACCCTCTCAGGCCGGCT3770.27227293737000235No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGGGGACCTTCCTCTCAGATCCCCT3600.25999537785994914No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT3520.2542177027963947No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGGCCACCCTCTCAGGCCGGCT3430.247717818349896No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT3430.247717818349896No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGTCGGCT3430.247717818349896No Hit
CTGGGCCGTGTCTCAGTCCNAATCTGGCTGGTCGGTCTCTCAACCCAGCT3140.22677374624451124No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGCCGGCT3070.2217182805639011No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCGCCCTCTCAGGCCGGCT2910.2101629304367922No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT2770.20005199907557197No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTTAACCTCTCAGTCCGGCT2750.19860758030968337No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGTTCAACCTCTCAGTTCGGCT2710.19571874277790619No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGCCGATCACCCTCTCAGGTCGGCT2690.19427432401201758No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCGGTCTCTCAACCCGGCT2610.18849664894846313No Hit
CTGGACCGTATCTCAGTTCCAATGTGGCCGTTCGACCTCTCAGTCCGGCT2430.17549688005546568No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCAACCTCTCAGTCCGGCT2420.1747746706725214No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGCCGTTCATCCTCTCAGACCGGCT2380.17188583314074415No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCAACCTCTCAGTTCGGCT2370.17116362375779987No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGATCACCCTCTCAGGTCGGCT2340.16899699560896697No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCAGTCTCTCAACTCGGCT2320.16755257684307834No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCCGGTCACCCTCTCAGGTCGGCT2270.16394152992835684No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCGCCCTCTCAGGCCGGCT2220.1603304830136353No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT2220.1603304830136353No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGGTTAACCTCTCAGTCCCCCT2190.1581638548648024No Hit
TTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT1960.14155303905708344No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGGGACCTTCCTCTCAGATCCCCT1930.13938641090825052No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGATCGGTCTCTCAACCCGGCT1880.135775363993529No Hit
TTGGACCGTGTCTCAGTTCCAGTGTGGGGGACCTTCCTCTCAGAACCCCT1880.135775363993529No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCATCCTCTCAGACCGGCT1700.12277559510053154No Hit
CTGGACCGTGTTTCAGTTCCAGTGTGGCCGATCATCCTCTCAGATCGGCT1640.11844233880286574No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGGTCGGTCTCTCAACCCGGCT1610.11627571065403282No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTCCATCCTCTCAGACCGGCT1500.10833140744164549No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCAACCTCTCAGTTCGGCT1480.10688698867575687No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCCGCT1480.10688698867575687No Hit
CTGGACCGTGTCTCAGTTCCAATGTGGCCGGCCGACCTCTCAGTCCGGCT1470.10616477929281257No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTTAACCTCTCAGTCCGGCT1450.10472036052692398No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGGTCGCCCTCTCAGGCCGGCT1400.10110931361220245No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)