FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137362
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT1657712.068111996039661No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT133349.707197041394272No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGGGACCTTCCTCTCAGAACCCCT90406.581150536538489No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT76035.535009682444927No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT71025.170279990099154No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT53963.928306227340895No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT38032.7685968462893666No Hit
CTGGTCCGTGTCTCAGTACNAGTGTGGGGGGTTAACCTCTCAGTCCCCCT30202.198570201365734No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT29702.1621700324689503No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT29112.119217833170746No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCAGCCTCTCAGCTCGGCT21821.58850337065564No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGGTCAACCTCTCAGTCCGGCT17101.2448857762700019No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGGTTAACCTCTCAGTCCCCCT16401.1939255398145048No Hit
TTGGTCCGTGTCTCAGTACNAATGTGGGGGACCTTCCTCTCAGAACCCCT15831.1524293472721714No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGAAAACCTCTCAGTCCCCCT15281.1123891614857093No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGTTCATCCTCTCAGACCGGCT14381.0468688574714986No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGATCAGCCTCTCAGCTCGGCT13710.9980926311498085No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGACCTTCCTCTCAGAACCCCT12420.9041801953961067No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTGAGCCTCTCAGCTCGGCT12340.8983561683726212No Hit
CTGGACCGTATCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT12130.8830680974359721No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT11130.8102677596424048No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCAACCTCTCAGTCCGGCT10760.7833316346587849No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT10390.7563955096751649No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTTCGGCT9810.7141713137548958No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT9660.7032512630858607No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT9580.6974272360623753No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT9520.6930592157947613No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT9360.6814111617477905No Hit
TTGGTCCGTGTCTCAGTACNAATGTGGGGGGTTAACCTCTCAGTCCCCCT9130.66466708405527No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTAGTGCCTCTCAGCTCGGCT8980.653747033386235No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCAACCTCTCAGTTCGGCT8820.6420989793392642No Hit
CTGGTCCGTGTCTCAGTACNAGTGTGGGGGGAAAACCTCTCAGTCCCCCT8800.6406429725833928No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGTTCATCCTCTCAGACCGGCT8020.5838587091044102No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTGAGCCTCTCAGCTCGGCT7260.528530452381299No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGGGACCTTCCTCTCAGATCCCCT6950.5059623476652931No Hit
CTGGACCGTATCTCAGTTCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT6660.48485024970515866No Hit
CTGGTCCGTGTCTCAGTACNAGTGTGGGGGACCTTCCTCTCAGAACCCCT6650.48412224632722295No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCACCCTCTCAGGTCGGCT6360.46301014836708837No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCCGATCACCCTCTCAGGTCGGCT6160.448450080808375No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTTCGGCT6120.44553806729663226No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCCGATCAACCTCTCAGTTCGGCT5480.39894585110874914No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCTGGTCATCCTCTCAGACCAGCT5480.39894585110874914No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCCGATCAACCTCTCAGTCCGGCT5430.3953058342190708No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGCCGGCT5430.3953058342190708No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCATCCTCTCAGACCGGCT5280.38438578355003566No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTAGTGCCTCTCAGCTCGGCT5140.37419373625893626No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTGAACCTCTCAGTCCCCCT5090.37055371936925785No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGTCGGCT4930.35890566532228707No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGGGGACCTTCCTCTCAGAACCCCT4870.35453764505467306No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT4680.34070558087389524No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGGACATCCTCTCAGACCGGCT4120.2999373917094975No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGTTCAACCTCTCAGTCCGGCT3880.28246531063904134No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCGACCTCTCAGTCCGGCT3620.26353722281271386No Hit
CTGGTCCGTATCTCAGTACCAGTGTGGGGGATAATCCTCTCAGACCCCCT3460.2518891687657431No Hit
CTGGACCGTGTTTCAGTTCCAGTGTGGCCGATCATCCTCTCAGATCGGCT3450.2511611653878074No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGCCGGCT3310.24096911809670796No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTTCGGCT2970.21621700324689508No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCAACCTCTCAGTCCGGCT2820.20529695257785996No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGGGGACCTTCCTCTCAGAACCCCT2780.20238493906611726No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGATCGTCCTCTCAGACCAGCT2710.19728891542056753No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCACCCTCTCAGGCCGGCT2670.19437690190882484No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT2470.17981683435011137No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT2430.1769048208383687No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT2390.173992807326626No Hit
CTGGTCCGTGTCTCAGTACNAGTGTGGGGGGTGAACCTCTCAGTCCCCCT2380.17326480394869032No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGGACATCCTCTCAGACCGGCT2330.16962478705901196No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGATCGGTCTCTCAACCCGGCT2180.15870473638997684No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGCGACCTTCCTCTCAGAACCCCT2150.15652072625616983No Hit
CTGGACCGTGTTTCAGTTCNAGTGTGGCCGATCATCCTCTCAGATCGGCT2090.1521527059885558No Hit
CTGGTCCGTGTCTCAGTACNAGTGTGGGGGGTTAACCTCTCAGTTCCCCT2080.15142470261062013No Hit
CTGGGCCGTGTCTCAGTCCCAATGCGGCCGATCAACCTCTCAGTTCGGCT2040.1485126890988774No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCGACCTCTCAGTCCGGCT1960.14268866207539202No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT1940.14123265531952067No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCTGATCGTCCTCTCAGACCAGCT1910.13904864518571367No Hit
CTGGTCCGTATCTCAGTACNAGTGTGGGGGATAATCCTCTCAGACCCCCT1740.12667258776080723No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGGTCAACCTCTCAGTTCGGCT1680.12230456749319316No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT1680.12230456749319316No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGGTGAACCTCTCAGTCCCCCT1670.1215765641152575No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCGGCCTCTCAGCCCGGCT1670.1215765641152575No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCCGTTCAACCTCTCAGTCCGGCT1640.11939255398145047No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGCGACCTTCCTCTCAGAACCCCT1620.11793654722557913No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTCCGCCCTCTCAGGCCGGCT1550.11284052358002941No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT1470.10701649655654402No Hit
TTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT1470.10701649655654402No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCATCCTCTCAGACCGGCT1450.10556048980067269No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTCAACCTCTCAGTCCCCCT1420.10337647966686565No Hit
TTGGTCCGTGTCTCAGTACNAATGTGGGGGACCTTCCTCTCAGATCCCCT1420.10337647966686565No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCGGACCTTCCTCTCAGAACCCCT1400.1019204729109943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCT34850.0292.337137
GACCGTA2400.0292.33714
CGTAGCT108.689743E-4292.33717
GGACCGT45000.0292.012273
CGTGTCT99950.0291.752147
GTGTCTC99850.0291.751568
GACCGTG41650.0291.63524
TGGACCG45150.0290.71842
TGTCTCA100250.0290.441659
TTGGACC30200.0290.40111
CTGGACC15000.0290.388181
GGCCGTG35450.0290.275454
CCGTGTC101050.0290.167366
GCCGTGT35550.0289.870125
CTGGTCC16800.0289.726931
TGGTCCG25300.0289.448362
CCGTATC35200.0289.430336
GTCCGTG24500.0289.354064
GCCGTAT32050.0289.144655
GTATCTC35250.0289.019788
Produced by FastQC (version 0.11.5)