FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005103596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146277
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA4656531.83343929667685No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA135769.281021623358422No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT82855.66391161973516No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAGCACAG76045.198356542723737No Hit
AGTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA41882.863061178449107No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGTTT32622.2300156552294617No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG27231.8615366735713748No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT22861.5627884082938537No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTTCTT22511.5388612016926788No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAACTGTTT19611.3406072041400903No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGAGA18611.2722437567081633No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGTTT16611.135516861844309No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGACGG16591.1341495928956704No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGAGGG15221.0404916699139304No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCGAA13530.9249574437539737No Hit
AGTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA12220.8354013276181491No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACCTT11900.8135250244399324No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGT11600.7930159902103544No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTT10440.7137143911893189No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCGAGCAGCA9770.6679108814099277No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC9610.6569727298208194No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCGAGCACTT9360.6398818679628376No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT8440.5769874963254648No Hit
AATGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA8270.5653657102620371No Hit
AGTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAGCACAG6850.46828961490870064No Hit
GATGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAGCGGAGTTTTGT5670.38762074693902665No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACGAGGCC5650.3862534779903881No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTAAGA5270.3602753679662558No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTT5100.3486535819028282No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGTAGCACAG4970.3397663337366777No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACATT4520.30900278239231044No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGATA4350.2973809963288829No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGGGGATGCCG4200.2871264792140938No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGATGAAACCG4150.2837083068424975No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGACATAG4070.2782392310479433No Hit
GATGAACGCTGGCGGCGTGCTTAATACATGCAAGTCGAGCGAAGCGCTTT3960.2707192518304313No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATAGAC3870.26456654156155784No Hit
ATTGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT3860.26388290708723855No Hit
GATGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA3820.26114836918996154No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGATGATGAA3730.25499565892108805No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATTACT3660.2502102176008532No Hit
GACGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAGCGAATGACGAA3650.24952658312653392No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGATCCCAG3630.24815931417789533No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTC3590.2454247762806183No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAATGTA3530.24132296943470266No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAACGGA3370.2303848178455943No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCGCTGT3270.22354847310240158No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGTTAAACCG3160.21602849388488965No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGTACCATGG3050.20850851466737763No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAATGATGAA2870.19620309412963077No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAGTCAT2830.1934685562323537No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGAATCTTT2770.18936674938643805No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGAGCTGCTT2650.18116313569460682No Hit
GACGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA2640.18047950122028752No Hit
ACTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA2630.17979586674596826No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGCTTG2580.17637769437437192No Hit
GATGAACGCTGGCGGCGTGCTTAACACATTCAAGTCGAACGGAGTCTTTT2550.1743267909514141No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA2530.17295952200277556No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGTTACCC2520.1722758875284563No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAAGTGAAGA2470.16885771515685993No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGAGCTGTTT2450.1674904462082214No Hit
AATGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA2400.16407227383662504No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATATT2390.16338863936230574No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTTTTCTT2360.16133773593934794No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCGCCTT2310.15791956356775158No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCATTTT2180.14903231540160106No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCTTTCC2170.14834868092728182No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG2150.14698141197864326No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGT1980.13535962591521564No Hit
ATTGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGTTT1970.13467599144089637No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAAGTCTTTA1920.13125781906930004No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGCAGGGTAACATGA1720.1175851295829146No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGGGTTTGG1690.11553422615995679No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTATGA1680.11485059168563753No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAATGGA1680.11485059168563753No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGATTCTCTTC1660.11348332273699899No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGGATGCG1610.11006515036540263No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGTGCTCA1480.1011779021992521No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)