FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005103598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125516
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG104568.330412059020365No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGATA90767.230950635775518No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG62224.957136938716976No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA54674.355620000637369No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGCGG46733.7230313266834507No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT33142.6403008381401576No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT28422.2642531629433695No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTCT26552.1152681729819305No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAACC25602.0395806112368144No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG23801.896172599509226No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGCGGGGCAGCATGT22291.7758692118933044No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA22191.7679021001306607No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA21321.6985882277956597No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTACAT20711.6499888460435324No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATCA20061.5982026195863475No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAGGACAA18971.51136110137353No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA17911.4269097166895057No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTG17221.3719366455272635No Hit
GATGAACGCTAGCTATAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA17011.3552057108257114No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTTTAAC15651.2468529908537556No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA14541.1584180502884096No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGGAGTTAATT14131.1257528920615698No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAACC14101.1233627585327766No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT12931.0301475509098441No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG12631.0062462156219127No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTGTTT11900.948086299754613No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAATC11780.9385257656394403No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTC11390.9074540297651295No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTTTCA10740.8556678033079449No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGTGAAGAGG10200.8126453997896682No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG9370.7465183721597246No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGCTG9350.7449249498071959No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC8860.7058861021702412No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGTGG8110.6461327639504127No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC7990.6365722298352402No Hit
GATGAACGCCGGCGGCGTGCCTAATACATGCAAGTCGAGCGGGGCCCGCA7620.6070939163134581No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAT7570.6031103604321362No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGTT7250.5776156027916759No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGCGGGGCAGCATGA6960.5545109786800089No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGCGG6760.5385767551547213No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCCTG6610.5266260875107556No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTA6350.5059115969278817No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGA6240.4971477739889736No Hit
GATGAACGCTGGCGGCGCATTTAAGACATGCAAGTCGAACGAAAACGAAC6160.49077408457885846No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGAGAGAAGAG5920.47165301634851337No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTC5180.4126963893049492No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG5150.41030625577615604No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT5130.4087128334236273No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGCGAAAGTGG5100.4063226998948341No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAACT5090.40552598871856976No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAC4880.38879505401701775No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAGGGTACTT4560.3633002963765576No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA4430.3529430510851206No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGG4350.34656936167500557No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGTACGAAGTACCCC4340.34577265049874123No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGGATTTAA4240.3378055387360974No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAAG4240.3378055387360974No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCTATC3980.3170910481532235No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGACTTGGAT3430.27327193345868256No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAAGTGGCCC3400.2708817999298894No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGGCCGAGG3300.2629146881672456No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT3250.25893113228592374No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC3230.25733770993339494No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG3210.2557442875808662No Hit
GACGAACGCTGGCGGCACGCCTAACACATGCAAGTCGAACGGACGAGGAG2900.23104624111667035No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT2850.22706268523534848No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCTTGAT2680.213518595238854No Hit
ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGAACGGCAGCGCGG2630.2095350393575321No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG2560.20395806112368142No Hit
GATTAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGGAACACCCC2520.20077121641862394No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATGCCG2360.18802383759839383No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGACATCT2330.1856337040696007No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGGTGAAGAGA2260.18005672583575003No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGTACGCGAGGGCCC2150.17129290289684185No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTCAACC2130.16969948054431305No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA2020.16093565760540487No Hit
GATGAACGCTAGCTAGAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA1870.1489849899614392No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAAGGGACCT1810.1442047229038529No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTGAAGCT1710.13623761114120908No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAACGGA1660.1322540552598872No Hit
GATGAACGCTGGCGGCGTGCTTAACACATTCAAGTCGAACGAAGAAGATT1660.1322540552598872No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTCAAGA1580.12588036584977214No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAAGTGTTC1580.12588036584977214No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT1480.11791325408712834No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTT1440.11472640938207082No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCTGC1420.11313298702954205No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGG1410.11233627585327767No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA1380.10994614232448453No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTT1330.10596258644316263No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATTACT1310.10436916409063386No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGAAG1300.10357245291436948No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGATGGCCC1280.10197903056184071No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAA1260.10038560820931197No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)