FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125516
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT1803514.368686063928104No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT117689.375697122279231No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGGGACCTTCCTCTCAGAACCCCT97617.77669779151662No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT63315.043978456929794No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT44663.5581121131967235No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT38953.1031900315497625No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT37382.9781063768762546No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGGGGGTCTTCCTCTCAGAACCCCT26272.092960260046528No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGCCGGCT24971.9893878071321585No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT22311.777462634245833No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT21701.728863252493706No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGTCGGCT21101.6810605819178432No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGGGGACCTTCCTCTCAGAACCCCT18501.4739156760891041No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT17491.3934478472864018No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT15981.2731444596704804No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT15871.264380636731572No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGTTCATCCTCTCAGACCGGCT15151.2070174320405367No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGGGGGTCTTCCTCTCAGAACCCCT14901.187099652633927No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGTCGGCT12641.007042926798177No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT12360.9847350138627744No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCACCCTCTCAGGTCGGCT10340.8237993562573696No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGGGGACCTTCCTCTCAGAACCCCT10270.8182223780235189No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGATCAACCTCTCAGTTCGGCT9410.7497052168647822No Hit
ATGGGCCGTGTCTCAGTCCCATTGTGGGGGGCCTTCCTCTCAGAACCCCT9240.7361611268682877No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCTACCTCTCAGTACGGCT8940.7122597915803562No Hit
TTGGTCCGTGTCTCAGTACNAATGTGGGGGACCTTCCTCTCAGAACCCCT8730.6955288568788043No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGACCTTCCTCTCAGAACCCCT8570.6827814780585741No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTAGAGTCTCTCAACTCGGCT7940.6325886739539183No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGTTCATCCTCTCAGACCGGCT7780.6198412951336881No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGGGACCTTCCTCTCAGATCCCCT7380.5879728480831129No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTTCGGCT7290.5808024474967335No Hit
CTGGTCCGTGTCTCAGTACNAGTGTGGGGGGTTAACCTCTCAGTCCCCCT6420.5114885751617324No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT6270.49953790751776667No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT5670.4517352369419038No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT5570.44376812517926006No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTTCGGCT5410.43102074635902987No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT5060.40313585518977657No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCTACCTCTCAGTACGGCT5030.4007457216609835No Hit
CTGGACCGTGTCTCAGTTCCAATGTGGCCGGCCGACCTCTCAGTCCGGCT4960.39516874342713276No Hit
CTGGTCCGTGTCTCAGTACNAGTGTGGGGGACCTTCCTCTCAGAACCCCT4770.38003123107810954No Hit
ATGGGCCGTGTCTCAGTCCNATTGTGGGGGGCCTTCCTCTCAGAACCCCT4720.37604767519678767No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTTCGGCT4620.36808056343414386No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGCCAGCCTCTCAGCTCGGCT4560.3633002963765576No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTAGAGTCTCTCAACTCGGCT4420.35214633990885624No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCAACCTCTCAGTCCGGCT3970.31629433697695913No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGTCGGCT3860.30753051403805093No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT3710.29557984639408524No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGGTCAACCTCTCAGTCCGGCT3590.28601931227891264No Hit
TTGGACCGTGTTTCAGTTCCAATGTGGCCGATCATCCTCTTAAATCGGCT3500.27884891169253323No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCGTCCTCTCAGACCGGCT3270.2605245546384525No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTTAACCTCTCAGTTCGGCT3200.2549475764046018No Hit
CTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT3150.25096402052327993No Hit
CTGGACCGTGTCTCAGTTCNAATGTGGCCGGCCGACCTCTCAGTCCGGCT3080.24538704228942923No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGCCAGCCTCTCAGCTCGGCT2870.22865610758787724No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGTCGGCT2830.22546926288281974No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACTCTCTCAAGCCGGCT2740.2182988622964403No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCT2690.2143153064151184No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCACCCTCTCAGGTCGGCT2580.2055514834762102No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCAACCTCTCAGTCCGGCT2550.20316134994741705No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGCCGGCT2440.19439752700850887No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTTAACCTCTCAGTCCGGCT2440.19439752700850887No Hit
CTGGGCCGTGTCTCAGTCCCAATCTGGCCGGTCGGTCTCTCAACCCGGCT2420.1928041046559801No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT2380.1896172599509226No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGCGACCTTCCTCTCAGAACCCCT2370.1888205487746582No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCAACCTCTCAGTTCGGCT2360.18802383759839383No Hit
ATGGACCGTGTCTCAGTTCCATTGTGGGGGACCTTCCTCTCAGAACCCCT2170.17288632524937061No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT2110.1681060581917843No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGTCCGATCAGCCTCTCAGCTCGGAT2050.16332579113419804No Hit
TTGGACCGTGTTTCAGTTCNAATGTGGCCGATCATCCTCTTAAATCGGCT2030.16173236878166927No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT1740.13862774467000222No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT1740.13862774467000222No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCGTCCTCTCAGACCGGCT1700.1354408999649447No Hit
CTGGACCGTATCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT1690.1346441887886803No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTTAACCTCTCAGTTCGGCT1690.1346441887886803No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGCCGGCT1670.13305076643615157No Hit
CTGGACCGTCTCTCAGTTCNAATGTGGCCGATCACCCTCTCAGGTCGGCT1640.1306606329073584No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCAACCTCTCAGCTCAGCT1620.12906721055482964No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCTACCTCTCAGTACGGCT1560.1242869434972434No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCCGATCAACCTCTCAGTCCGGCT1550.123490232320979No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTTAACCTCTCAGTCCGGCT1460.11631983173459956No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGCGACCTTCCTCTCAGAACCCCT1450.11552312055833518No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCTGGTCGTCCTCTCAGACCAGCT1420.11313298702954205No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGGTTAACCTCTCAGTCCCCCT1400.11153956467701329No Hit
ATGGACCGTGTCTCAGTTCNATTGTGGGGGACCTTCCTCTCAGAACCCCT1390.1107428535007489No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCAACCTCTCAGTCCGGCT1370.10914943114822015No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCCGGTCACCCTCTCAGGTCGGCT1330.10596258644316263No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACTCTCTCAAGCCGGCT1300.10357245291436948No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCGGACCTTCCTCTCAGAACCCCT1300.10357245291436948No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTGT108.6859113E-4292.37012295
TTGGACC34400.0292.370121
GTGTCTC107850.0292.099038
ACCGTGT41550.0292.018285
CGTGTCT107950.0291.963877
TGTCTCA107900.0291.963659
CCGTGTC108400.0291.291266
GGCCGTG61200.0291.17584
GACCGTG41800.0290.62154
GCCGTGT61200.0290.220345
CTGGGCC52750.0290.15311
TGGACCG43500.0289.681642
GGACCGT43550.0289.013433
TTGGGCC20950.0288.881231
CGTATCT14800.0287.431467
GTATCTC15050.0283.628178
GCCGTAT14400.0283.233555
TTGGTCC3150.0283.088531
CCGTATC15150.0280.79116
GTCCGTG7150.0278.05834
Produced by FastQC (version 0.11.5)