FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005103600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144936
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAGGAAAA136049.386211845228239No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCTTGAT83805.781862339239388No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG73415.064994204338467No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATGAAGTTA69494.794529999448032No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTGAAGCT66204.567533256057846No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTT51753.570541480377546No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA46153.1841640448197825No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGACGGCCC37582.592868576475134No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTTCTT30212.084368272892863No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG29702.0491803278688523No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCTATC27611.9049787492410444No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT27001.8628912071535022No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGA26491.8277032621294917No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA24291.675912126731799No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCTGC23691.6345145443506097No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGG21581.4889330463100954No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA17181.18535077551471No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA17111.1805210575702378No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC16991.1722415410940001No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGT14410.9942319368548878No Hit
AACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGAGAGAATTG14190.9790528233151183No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGATGCAT12600.8693492300049677No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGCTAGGA10760.7423966440359883No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGTT10180.7023789810675056No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA9720.6706408345752608No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGACA9570.6602914389799636No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGACTTGT9540.6582215598609041No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGCCTT9280.6402826074957222No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTAACCCA8320.5740464756858199No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGTTT8220.5671468786222884No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGGGGATATTG7730.5333388530109842No Hit
GACGAACGCTGGCGGCGTGCTTAATACATGCAAGTCGAACGGAGTTAAGG7610.5250593365347463No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCCCTC7470.5153999006458023No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACCTT7280.5022906662250924No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGTACGAAGTGGCCC7170.49470110945520784No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGATA6630.4574432853121378No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGT6300.43467461500248383No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTG5570.38430755643870396No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAACGGA5460.37671799966881936No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT5410.37326820113705356No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAACCACTTC5220.3601589667163438No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT5180.3573991278909312No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG5140.35463928906551856No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGT5130.35394932935916545No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG4930.3401501352321024No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTT4880.33670033670033667No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTTC4860.3353204172876304No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT4780.32980073963680523No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC4670.3222111828669206No Hit
GATGAACGCTGGCGGCGTGCTTAACACATTCAAGTCGAACGGAGTTCAAT4600.31738146492244856No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTCGTTT4540.3132417066843296No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA4530.3125517469779765No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGATCCCAG4450.30703206932715127No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGATCCATC4230.291852955787382No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGAATCTTT4010.2766738422476127No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAGGAAAA3920.2704642048904344No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGACAAGG3860.2663244466523155No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTCGGCC3760.25942484958878403No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG3650.2518352928188994No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTA3590.2476955345807805No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT3580.2470055748744273No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGATTACA3550.2449356957553679No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGATTAAGACG3470.23941601810454272No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGATTCTCTTC3460.23872605839818953No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGATGTGC3450.2380360986918364No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTGAAGCT3450.2380360986918364No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGG3390.2338963404537175No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTT3370.2325164210410112No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCTGCTT3370.2325164210410112No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACATGT3360.23182646133465806No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGAGA3300.22768670309653918No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTATTT3010.20767787161229784No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAGTTA2930.20215819396147264No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCCTT2910.20077827454876634No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCTTGAT2890.19939835513606002No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTGAAA2860.1973284760170006No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAACACCTT2790.1924987580725286No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC2760.1904288789534691No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGATGTAC2720.18766904012805652No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACGAGAGG2700.18628912071535023No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGCTTT2680.1849092013026439No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAATATT2680.1849092013026439No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGATCTTCTTC2570.17731964453275928No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTCA2500.17248992658828724No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG2490.1717999668819341No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGACGGACTT2480.17111000717558095No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGCTTT2480.17111000717558095No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATTACT2480.17111000717558095No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCTATCT2420.16697024893746207No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAT2400.16559032952475575No Hit
AGTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA2300.15869073246122428No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGATCGGCT2300.15869073246122428No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA2250.15524093392945854No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATGAAGTTA2240.15455097422310538No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG2230.15386101451675221No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTAAGACG2220.15317105481039908No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGC2170.14972125627863334No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT2150.14834133686592701No Hit
GACGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA2120.1462714577468676No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTT2040.1407517800960424No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCAGGA1880.12971242479439202No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCACTTT1790.12350278743721366No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCACCCC1780.12281282773086051No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGACGATGAA1770.12212286802450738No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCGAA1730.11936302919909476No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTATGT1710.11798310978638848No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA1680.11591323066732903No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACTTT1660.11453331125462272No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT1650.11384335154826959No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAATGAT1630.11246343213556328No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT1610.11108351272285698No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGA1530.1055638350720318No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTGTTGC1500.10349395595297235No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGTTATTT1460.10073411712755975No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)