FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94510
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT92969.835996190879271No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT81498.622368003385885No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT61056.459633901174479No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT59596.305152893873664No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCACCCTCTCAGGTCGGCT51205.417416146439531No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT46374.906359115437519No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGGGACCTTCCTCTCAGAACCCCT33403.534017564278912No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT33193.5117976933657813No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGTTCATCCTCTCAGACCGGCT30963.2758438260501532No Hit
TTGAGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT22602.3912813458893236No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT22302.3595386731562797No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGTTCATCCTCTCAGACCGGCT16651.7617183366839488No Hit
CTGGACCGTATCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT16131.7066977039466722No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT14731.5585652311924663No Hit
TTGAGCCGTGTCTCAGTCCNAATGTGGCCGATCACCCTCTCAGGTCGGCT13111.387154798434028No Hit
CTGGTCCGTGTCTCAGTACNAGTGTGGGGGGTTAACCTCTCAGTCCCCCT12181.2887525129615913No Hit
CTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT11401.2062215638556766No Hit
CTGGACCGTATCTCAGTTCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT9851.0422177547349487No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT8920.9438154692625119No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGTCGGCT8660.9163051528938737No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT7210.7628822346841604No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTTCGGCT6690.707861601946884No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTTCGGCT6690.707861601946884No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT6520.6898740873981589No Hit
CTGGACCGTCTCTCAGTTCNAATGTGGCCGATCACCCTCTCAGGTCGGCT5960.6306210982964766No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCACCCTCTCAGGTCGGCT5100.5396254364617501No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGCCGGCT4990.5279864564596339No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT4670.4941276055443869No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCAGCCTCTCAGCTCGGCT4610.48777907099777806No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGAAAACCTCTCAGTCCCCCT4420.4676753782668501No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGCCGGCT4020.4253518146227912No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGTCGGCT3850.40736430007406627No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT3600.3809120727965295No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT3590.379853983705428No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGGTTAACCTCTCAGTCCCCCT3570.37773780552322506No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCACCCTCTCAGGCCGGCT3500.3703311818855148No Hit
TTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT3370.3565760237011956No Hit
CTGGGCCGTGTCTCAGTCCCAATCTGGCCGGTCGGTCTCTCAACCCGGCT3360.3555179346100942No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT3360.3555179346100942No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT3330.35234366733678973No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCGCCCTCTCAGGCCGGCT3130.33118188551476035No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT3040.32165908369484714No Hit
CTGGGCCGTGTCTCAGTCCCAATGCGGCCGATCAACCTCTCAGTTCGGCT2900.30684583641942653No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCCGGTCACCCTCTCAGGTCGGCT2850.30155539096391915No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTTCGGCT2740.289916410961803No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCACCCTCTCAGGTCGGCT2440.25817373822875883No Hit
CTGGTCCGTGTCTCAGTACNAGTGTGGGGGGAAAACCTCTCAGTCCCCCT2440.25817373822875883No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCATCCTCTCAGACCGGCT2400.25394138186435294No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT2340.24759284731774417No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCGCCCTCTCAGGCCGGCT2280.24124431277113534No Hit
TTGGTCCGTGTCTCAGTACNAATGTGGGGGGTTAACCTCTCAGTCCCCCT2280.24124431277113534No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGGGACCTTCCTCTCAGATCCCCT2240.23701195640672945No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCGACCTCTCAGTCCGGCT2210.23383768913342504No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGGGATCTTCCTCTCAGAACCCCT2090.22114062004020738No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGATCAGCCTCTCAGCTCGGCT2090.22114062004020738No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT2080.2200825309491059No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCAACCTCTCAGTCCGGCT2080.2200825309491059No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGTTCATCCTCTCAGACCGGCT2050.2169082636758015No Hit
CTGGGCCGTGTCTCAGTCCNAATCTGGCCGGTCGGTCTCTCAACCCGGCT1950.20632737276478677No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCGCCCTCTCAGGCCGGCT1930.20421119458258383No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGGTCAACCTCTCAGTCCGGCT1910.20209501640038094No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCAACCTCTCAGTTCGGCT1880.19892074912707652No Hit
TTGGACCGTCTCTCAGTTCNAATGTGGCCGATCACCCTCTCAGGTCGGCT1840.1946883927626706No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT1690.17881705639614856No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTGAACCTCTCAGTCCCCCT1640.1735266109406412No Hit
CTGGGCCGTGTCTCAGTCCNAATGCGGCCGATCAACCTCTCAGTTCGGCT1610.1703523436673368No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT1600.16929425457623534No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCGCCCTCTCAGGCCGGCT1590.16823616548513387No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGGACATCCTCTCAGACCGGCT1490.15765527457411915No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT1490.15765527457411915No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT1430.15130674002751032No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT1350.14284202729869855No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT1330.1407258491164956No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCGACCTCTCAGTCCGGCT1320.13966776002539413No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGGTCGCCCTCTCAGGCCGGCT1260.1333192254787853No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGCCGATCACCCTCTCAGGTCGGCT1220.12908686911437942No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGCCGTTCATCCTCTCAGACCGGCT1200.12697069093217647No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT1190.125912601841075No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGGGATCTTCCTCTCAGAACCCCT1180.12485451274997354No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACTCTCTCAAGCCGGCT1160.12273833456777061No Hit
TTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT1120.11850597820336473No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTACATCCTCTCAGATCGGCT1110.11744788911226325No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTAGAGTCTCTCAACTCGGCT1100.11638980002116178No Hit
CTGGTCCGTGTCTCAGTACNAGTGTGGGGGGTGAACCTCTCAGTCCCCCT1090.11533171093006031No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCT1060.1121574436567559No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGGACATCCTCTCAGACCGGCT1040.11004126547455295No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGTTCATCCTCTCAGATCGGCT1020.10792508729235001No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGCCACCCTCTCAGGCCGGCT1010.10686699820124854No Hit
TTGGTCCGTGTCTCAGTACNAATGTGGGGGACCTTCCTCTCAGAACCCCT990.10475082001904559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTC2750.0292.366674
GACCGTA2950.0292.366674
AACGCCC157.482484E-6292.36667295
TGAGCTT108.6828275E-4292.36667295
GCCGTAT18750.0291.587045
CTGGGCC37350.0291.58391
CGTGTCT69450.0291.524727
GCCGTGT44650.0291.384465
CCGTGTC69600.0291.31656
GGACCGT25100.0291.201873
GTGTCTC69600.0291.106488
CGTATCT21750.0291.022467
CCGTATC21750.0291.022466
GGCCGTG40650.0290.92824
GACCGTG19350.0290.855744
ACCGTGT19300.0290.85185
TGTCTCA69750.0290.699
GTATCTC21800.0290.354958
TTGGACC12850.0290.091461
GTCCGTG5900.0289.888984
Produced by FastQC (version 0.11.5)