FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102577
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG1047210.208916228784231No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGCGGGGCAGCATGA64646.301607572847715No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG56655.522680522924242No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA47884.667713035085838No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA30783.000672665412324No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA27662.6965109137525958No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGCGG22572.2002983124872046No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG20191.968277489105745No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC17021.6592413504001873No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT15101.472064887840354No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT14361.3999239595620852No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT13031.2702652641430339No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA12581.2263957807305732No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA12251.1942248262281019No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGATTACT11731.1435312009514804No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGGAGGGACCC10501.0236212796240873No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACGAGGCC10351.0089981184866001No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGATTATT10160.9904754477124501No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG9820.9573296158008131No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGAGAGAAGAG9720.9475808417091551No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGT9470.9232089064800102No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGATTACA9370.9134601323883521No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGTACGCGAAAGGGA8670.8452187137467463No Hit
GATTAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT8430.8218216559267673No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGACTTTATA8070.7867260691967985No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTTTAAC7550.736032443920177No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCTTGAT7420.7233590376010217No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAAG7150.6970373475535452No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGTACGAAGGGGCCC7020.6843639412343898No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGACTTTACA6900.6726654123244002No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGATGGCCC6410.6248964192752762No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCTGC6310.6151476451836182No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATACTT6230.6073486259102917No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT6150.5995496066369654No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTATGA6060.5907757099544733No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATAAAG5870.572253039180323No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA5820.5673786521344941No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATACTG5470.5332579428136912No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTC5340.5205845364945358No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAGTTA5060.49328796903789346No Hit
ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGAACGGCAGCACAG5050.49231309162872766No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC5000.48743870458289873No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTA4920.4796396853095723No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAACC4870.4747652982637433No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT4590.44746873080710103No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTC4480.43674507930627726No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTTTAA4440.432845569669614No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG4210.4104233892588007No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATGCCG4090.39872486034881116No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGACATCT4030.3928755958938163No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTCT3850.375327802528832No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTT3840.3743529251196662No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTGTTT3740.3646041510280082No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGGGTTATAT3690.35972976398217926No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTCAAGA3400.3314583191163711No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA3050.2973376097955682No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTC2980.2905134679314076No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTGTTGA2940.28661395829474445No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGGATTTAA2870.27978981643058387No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGATATGC2800.2729656745664233No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC2730.26614153270226265No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCATTGA2730.26614153270226265No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAC2640.2573676360197705No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAT2630.25639275861060473No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAATCTT2610.25444300379227314No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAAGTGGCCC2500.24371935229144936No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGAAAGTT2330.2271464363356308No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA2320.226171558926465No Hit
GACGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGACTGATTC2300.2242218041081334No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTT2300.2242218041081334No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGATGAAA2300.2242218041081334No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTGAAA2290.2232469266989676No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATGAAGTTA2220.216422784834807No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACATT2200.2144730300164754No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGGAGACCT2000.19497548183315946No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTTGAACGGAGAAAGTT1990.19400060442399367No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGGAAGG1990.19400060442399367No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG1960.1910759721964963No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGCGGGGCAGCATGA1860.18132719810483833No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGGAGTGGCCC1850.18035232069567253No Hit
GATGAACGCTGGCGGCGTGCTTAACACATTCAAGTCGAACGGAGTTCAAT1770.17255330142234615No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCATCATGA1760.17157842401318035No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG1650.16085477251235658No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGATGCAT1630.15890501769402499No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCCTT1630.15890501769402499No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATACT1600.1559803854665276No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGAGG1570.15305575323903017No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATATT1550.1511059984206986No Hit
GATGAACGCTGGCGGCGTGCATAACACATTCAAGTCGAACGGACGATAGC1550.1511059984206986No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG1550.1511059984206986No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAGTTTAA1550.1511059984206986No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATTACT1520.1481813661932012No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGGCTG1510.14720648878403542No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATGCCG1440.14038234691987483No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTAAAGC1420.13843259210154324No Hit
GATTAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGGAACACCCC1380.13453308246488005No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGCTTA1370.13355820505571425No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAAGTGTTC1370.13355820505571425No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAAGTAGCCT1330.12965869541905106No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACATA1330.12965869541905106No Hit
AACGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGGAATATGAT1220.11893504391822729No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCATTAC1190.11601041169072988No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA1190.11601041169072988No Hit
GATTAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGGAGTGCCCC1190.11601041169072988No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTG1170.1140606568723983No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAGGGTACTT1170.1140606568723983No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAAGTGGCCT1160.1130857794632325No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGAAATCAC1160.1130857794632325No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGCGG1150.1121109020540667No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAACACCTT1110.1082113924174035No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGAATCTTT1100.1072365150082377No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGA1090.10626163759907192No Hit
GACGAACGCTGGCGGTGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA1070.10431188278074031No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT1070.10431188278074031No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGACTTGCC1070.10431188278074031No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGGTGGCCC1060.10333700537157452No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTGTTGC1060.10333700537157452No Hit
GACGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT1040.10138725055324294No Hit
GATGAACGCTGGCGGCGTGCCTAACACATTCAAGTCGAACGGAGCCATCG1030.10041237314407712No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGAA1030.10041237314407712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACGC2400.0295.421883
CGCTGGC68250.0295.421887
AAACGCT2400.0295.421884
ATAAACG2400.0295.421882
AACGCTG68300.0295.20565
GGGAACT157.2221883E-6294.98978295
GATGCAA157.2221883E-6294.98978295
GCTGGCT108.4546884E-4294.989758
ACGCTAG34050.0294.988076
ACGCTGG68450.0294.558726
CTAGCTA16800.0294.111829
GCTGGCG68550.0293.91398
CGCTAGC34200.0293.694277
GCTAGCT16850.0293.23918
TGAACGC63300.0292.8553
GCTAGCG17550.0290.787638
GATAAAC2450.0289.392851
GACGAAC36250.0288.902221
GAACGCT101100.0287.82454
ATTAACG1400.0284.87112
Produced by FastQC (version 0.11.5)