FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102577
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT96039.361747760219153No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT66556.487809157998383No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT62096.053013833510436No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT54725.334529182955244No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT40983.9950476227614375No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGGGGACCTTCCTCTCAGAACCCCT40723.9697008101231273No Hit
TTGGTCCGTGTCTCAGTACNAATGTGGGGGACCTTCCTCTCAGAACCCCT38583.761077044561646No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGGGACCTTCCTCTCAGAACCCCT34323.345779268257017No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT30953.017245581368143No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT22472.1905495383955467No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGGGGACCTTCCTCTCAGAACCCCT21652.1106095908439513No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT19031.8551917096425126No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGCCGGCT17261.6826384082201662No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT16051.564678241711105No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCAGCCTCTCAGCTCGGCT14661.429170281837059No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT13241.2907376897355156No Hit
ATGGGCCGTGTCTCAGTCCCATTGTGGGGGGCCTTCCTCTCAGAACCCCT13211.2878130575080182No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGTCGGCT12121.1815514199089465No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT11501.121109020540667No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT9990.9739025317566316No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCGACCTCTCAGTCCGGCT9790.9544049835733157No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT9730.9485557191183208No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCATCCTCTCAGACCGGCT9510.9271084161166734No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGATCAGCCTCTCAGCTCGGCT8770.8549674878384043No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT8570.8354699396550884No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCAACCTCTCAGTTCGGCT8000.7799019273326379No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCACCCTCTCAGGTCGGCT7500.731158056874348No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT7390.7204344053735242No Hit
ATGGGCCGTGTCTCAGTCCNATTGTGGGGGGCCTTCCTCTCAGAACCCCT7180.6999619797810426No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGTCGGCT6830.6658412704602397No Hit
CTGGACCGTGTTTCAGTTCCAGTGTGGCCGATCATCCTCTCAGATCGGCT6530.6365949481852656No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATTAACCTCTCAGTTCGGCT6470.630745683730271No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGGTCAACCTCTCAGTCCGGCT6000.5849264454994785No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTGAGCCTCTCAGCTCGGCT5440.5303333105861938No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCGACCTCTCAGTCCGGCT5300.5166850268578725No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCAACCTCTCAGTCCGGCT5190.5059613753570489No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCGCCCTCTCAGGCCGGCT5030.49036333681039607No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGCCGGCT4430.4318706922604483No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCT4410.4299209374421167No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCCGATCAACCTCTCAGTTCGGCT4220.41139826666796653No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT4200.40944851184963493No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTTCGGCT4200.40944851184963493No Hit
CTGGACCGTATCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT4130.40262436998547435No Hit
CTGGTCCGTGTCTCAGTACNAGTGTGGGGGGTTAACCTCTCAGTCCCCCT3920.3821519443929926No Hit
CTGGACCGTGTTTCAGTTCNAGTGTGGCCGATCATCCTCTCAGATCGGCT3790.36947853807383724No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGATTAACCTCTCAGTTCGGCT3750.365579028437174No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTGAGCCTCTCAGCTCGGCT3460.3373075835713659No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT3370.32853368688887374No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT3100.3022119968413972No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCACCCTCTCAGGTCGGCT3090.3012371194322314No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT2990.2914883453405734No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGCCAACCTCTCAGTCCGGCT2770.2700410423389259No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCGCCCTCTCAGGCCGGCT2610.25444300379227314No Hit
TTGGTCCGTGTCTCAGTACNAATGTGGGGGACCTTCCTCTCAGATCCCCT2540.24761886192811253No Hit
CTGGACCGTATCTCAGTTCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT2490.24274447488228357No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGGGACCTTCCTCTCAGATCCCCT2360.2300710685631282No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCTGGTCGTCCTCTCAGACCAGCT2280.2222720492898018No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGCCGATCACCCTCTCAGGTCGGCT2170.21154839778897805No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCAACCTCTCAGTCCGGCT2110.20569913333398326No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACTCTCTCAAGCCGGCT2060.20082474628815425No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTTCGGCT2050.19984986887898845No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGGGGACCTTCCTCTCAGATCCCCT1760.17157842401318035No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGCCAACCTCTCAGTCCGGCT1720.16767891437651716No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGTCCGATCAGCCTCTCAGCTCGGAT1710.16670403696735137No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCAACCTCTCAGTCCGGCT1640.15987989510319078No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCATCCTCTCAGACCGGCT1640.15987989510319078No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT1640.15987989510319078No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCCGGTCACCCTCTCAGGTCGGCT1590.1550055080573618No Hit
CTGGACCGTATCTCAGTTCCAATGTGGCCGTTCGACCTCTCAGTCCGGCT1530.14915624360236698No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGTCCGATCAGCCTCTCAGCTCGGAT1520.1481813661932012No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAGCCTCTCAGCTCGGCT1480.14428185655653802No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT1470.14330697914737223No Hit
CTGGACCGTTTCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT1440.14038234691987483No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT1400.13648283728321164No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT1370.13355820505571425No Hit
CTGGGCCGTGTTTCAGTCCCAATGTGGCCGATCAGCCTCTCAGCTCGGCT1330.12965869541905106No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGTCGGCT1330.12965869541905106No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT1320.12868381800988524No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCCGATCAACCTCTCAGTCCGGCT1320.12868381800988524No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTCCGCCCTCTCAGGCCGGCT1270.12380943096405626No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT1260.12283455355489048No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCAACCTCTCAGTTCGGCT1250.12185967614572468No Hit
CTGGGCCGTCTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT1200.11698528909989568No Hit
TTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT1200.11698528909989568No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCAACCTCTCAGTTCGGCT1140.1111360246449009No Hit
CTGGGCCGTGTCTCAGTCCCAATGCGGCCGATCAACCTCTCAGTTCGGCT1130.11016114723573511No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGACC108.685858E-4292.34473295
TGGACCG24800.0291.75532
CGTGTCT88150.0291.68147
TGTCTCA88300.0291.351479
CTGGACC13400.0291.253851
TTGGTCC12750.0291.198271
CCGTGTC88300.0291.18596
GTGTCTC88300.0291.18598
GGACCGT24800.0291.16593
TTGGACC11400.0291.062471
GACCGTG22400.0291.039584
GCCGTGT52900.0289.857855
CTGGGCC44850.0289.73741
TGGTCCG14350.0288.270232
ACCGTGT22650.0287.827245
CGTATCT12100.0287.512577
GGTCCGT14500.0286.29623
GGCCGTG53650.0285.80584
GGCCGTA10800.0285.577454
GTCCGTG14550.0285.312384
Produced by FastQC (version 0.11.5)