FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161926
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA115907.157590504304435No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT108776.717265911589244No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT91595.656287439941702No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA76604.73055593295703No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG60093.7109543865716437No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA58833.6331410644368414No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG58103.5880587428825517No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG46882.8951496362535973No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTACAT43372.6783839531637907No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT38432.3733063251114705No Hit
AACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGAGAGAATTG35572.1966824351864433No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGAATTTT35432.1880365105047987No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAT29931.8483751837258995No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTGTTT21531.3296197028272174No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC19691.2159875498684585No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG18611.1492904166100564No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA17411.0751824907673875No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGAG16861.0412163580894978No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGAATCTTT16701.0313353013104751No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC16631.0270123389696528No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCTATC16531.0208366784827636No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTTTCA14990.9257315069846721No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTT14960.9238788088386053No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTCT14500.8954707705989156No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGGAAGG12750.7873967120783568No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTCGGCC12190.752813013351778No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA11910.7355211639884885No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG11230.6935266726776429No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACCTT11220.692909106628954No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAGTTA11020.6805577856551759No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCACT10310.6367105961982634No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTGAAA10230.6317700678087522No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT9720.6002741993256179No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGGAATGTC9350.5774242555241283No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA8790.5428405567975495No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAGAAGAT8190.505786593876215No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGATCCCAG8170.5045514617788373No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACATA8080.4989933673406371No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAACGGA7950.4909650087076812No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG7610.4699677630522584No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGATCGGCT7440.459469140224547No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATTACT7430.45885157417585815No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGTTAAGGCG7340.45329347973765793No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTT6910.4267381396440349No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGGAGAACATT6730.41562195076763464No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT6480.40018279955041197No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGGAAGAGG6400.39524227116090066No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGG6380.3940071390635228No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGCTTA6240.38536121438187815No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAACACCTT6110.37733285574892234No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATATT6070.3748625915541667No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTC6030.3723923273594111No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT5840.36065857243432187No Hit
GATAAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGTTTTTC5690.35139508170398825No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCATGC5250.3242221755616763No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA5160.31866408112347616No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTCAAGA5110.3155762508800316No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA4890.3019897978088757No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGAGAGAAGAG4500.2779047219100083No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGATTT4150.2562899102058965No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAATATT4060.2507318157676964No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTGCTTGA4050.2501142497190074No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAATC4020.24826155157294072No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTAACCCG3950.24393858923211836No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGTGAATCT3930.2427034571347406No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTCGTTT3880.23961562689129604No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT3580.22108864543062878No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTAAAT3490.21553055099242865No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTG3480.21491298494373973No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGA3450.213060286797673No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTTGAACGGACTTAATC3370.20811975840816177No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGTGTTTA3340.20626706026209504No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAGTCAT3320.20503192816471721No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGTTATTT3170.19576843743438363No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT2990.1846522485579833No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA2900.1790941541197831No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT2860.17662388992502748No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGACGATGAA2790.17230092758420515No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCGCCTT2750.1698306633894495No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTCT2740.1692130973407606No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAATTT2730.1685955312920717No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGTTT2710.16736039919469387No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA2660.16427256895124934No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGATTCTCTTC2570.15871447451304918No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACATT2560.15809690846436025No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG2510.15500907822091572No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGG2450.1513036819287823No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGTAAGACG2410.14883341773402667No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACATGT2390.14759828563664884No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCAATTT2360.14574558749058214No Hit
GATGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGAGAGAATTG2300.14204019119844868No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTC2220.1370996628089374No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGCCTT2200.1358645307115596No Hit
GATGAACGCTGGCAGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTT2170.13401183256549287No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCGGAT2080.12845373812729272No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTT2080.12845373812729272No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG2040.1259834739325371No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTCA2000.12351320973778145No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGACATCT1990.12289564368909256No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATACT1980.12227807764040365No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGACATAC1980.12227807764040365No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGAGA1980.12227807764040365No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGATCTTCTTC1940.11980781344564802No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAATGAT1920.1185726813482702No Hit
GATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGGACGGGAAGTGGT1910.11795511529958129No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTC1890.11671998320220346No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACTTT1840.11363215295875893No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACGAGAGG1830.11301458691007005No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGC1820.11239702086138113No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTGAAGC1810.11177945481269222No Hit
GACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGAGAGAATTG1810.11177945481269222No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTTGAACGAGAATCTAC1790.11054432271531439No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGGCTG1740.10745649247186986No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTATGT1740.10745649247186986No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTA1730.10683892642318095No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCCTT1680.10375109617973641No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATGCCG1640.1012808319849808No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTACAT1640.1012808319849808No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGATGGA1640.1012808319849808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGGC134150.0295.44547
AAACGCT2700.0295.44544
ACGCTAG27250.0295.44546
CAAACCC108.459251E-4294.9892295
CGCTAGC27300.0294.904337
ACGCTGG134500.0294.676576
CTGGCGG134200.0294.549569
GCTGGCG134150.0294.54948
AACGCTG134700.0294.45845
TGAACGC95250.0294.359773
ACGAACG64100.0293.601782
CTAGCTA19200.0292.684579
TAAACGC2750.0290.07373
GACGAAC60500.0289.585331
CAGACAC3500.0286.5609295
GAACGCT163600.0286.415924
ATGAACG99100.0282.02962
CGAACGC67000.0281.11413
GATGAAC99650.0280.473021
GATAAAC2900.0280.163731
Produced by FastQC (version 0.11.5)