FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005103610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181206
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT4006822.11185060097348No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG165269.12000706378376No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT105155.802787987152743No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGAATTTT87254.8149619769764795No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCACGA52552.900014348310762No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGTTAGAC41112.268688674767944No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG34561.9072216151783055No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG31671.7477346224738695No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTATGC30091.6605410416873614No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGTTT29911.6506075957749742No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA29901.6500557376687306No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA25391.4011677317528117No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGA24941.3763341169718442No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT22701.2527179011732503No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCCACC20221.115857090824807No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACGAGAGG18951.0457711113318544No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGAATCTTT18901.0430118208006358No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCATCACTTT18671.03031908435703No Hit
AACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGAGAGAATTG18161.0021743209386003No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCATCACTTC17150.9464366522079843No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCGAGCACTT17090.9431255035705219No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGG16890.9320883414456475No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGTTACAC15700.8664172268026444No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAATCAT14240.785845943291061No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCCTT13430.7411454366853195No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGAGAGAAGAG13170.7267971259229826No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTCGGCC12150.6705075990861229No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAACACCTT11670.6440184099864242No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGTGCTCA11580.6390516870302309No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAATTTT10660.5882807412558083No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATATT10250.5656545588998156No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACGAGGAG9730.5369579373751421No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA9230.509365032062956No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCGAGCAGCA8580.473494255157114No Hit
GATGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGCTATTA8140.4492124984823902No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAAA7650.42217145127644784No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAATTT7480.4127898634703045No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCAATTA7190.39678597838923657No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTG7150.3945785459642617No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTGCTTGA7110.3923711135392868No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT7060.3896118230080682No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA6970.3846451000518747No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATACTT6480.3576040528459323No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCGATTT6150.3393927353398894No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACATGT5700.3145591205589219No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAA5580.3079368232839972No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGATTT5560.3068331070715098No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAATC5550.3062812489652661No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAGCTTC5540.30572939085902234No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC5530.30517753275277865No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGGAGATAAAT5440.3002108097965851No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCCCTC5310.2930366544154167No Hit
GATGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAGCGGAGGGAGAG5260.2902773638841981No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCGGGCAGCA5000.27592905312186133No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACATT5000.27592905312186133No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTACG4900.2704104720594241No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCATCACCTC4740.2615807423595245No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGAATTTT4430.2444731410659691No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGGGTTTGG4300.23729898568480073No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGAAG4220.23288412083485094No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGAATATT4170.23012483030363232No Hit
GATGAACGCTGGCGGCGTGCTTAATACATGCAAGTCGAACGAAGCACCTT3800.2097060803726146No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGTGAAAGTCC3500.19315033718530292No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA3150.17383530346677262No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTATTT3100.17107601293555402No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCAGTTT3040.16776486429809168No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCAATCA2980.16445371566062933No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGG2910.16059070891692326No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCGGTTT2910.16059070891692326No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTA2870.1583832764919484No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATTACT2840.15672770217321721No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG2810.15507212785448604No Hit
AGTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA2660.14679425626083023No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGATGATA2650.1462423981545865No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTTT2650.1462423981545865No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCACTTT2630.14513868194209906No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATACT2620.14458682383585533No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTT2590.14293124951712416No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGCGCTCA2580.14237939141088043No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGAGA2550.14072381709214926No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCACGA2380.13134222928600597No Hit
GATGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA2350.1296866549672748No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTT2300.1269273644360562No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTTCTT2130.11754577662991292No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCGCTTT2070.11423462799245059No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGATTT2070.11423462799245059No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCC2060.11368276988620686No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAGTCAT2060.11368276988620686No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTT1960.10816418882376962No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTATTT1950.10761233071752592No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG1940.1070604726112822No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCGACTG1940.1070604726112822No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCAATTT1820.10043817533635753No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)