FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184569
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA1991710.791086260422931No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAGCACAG1915410.377690728128776No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGAGA99825.408275495885008No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA67793.6728811447209444No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA59933.247024148150556No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT57563.118616885825897No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGGAAGG51472.788658983903039No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA45142.4456978149093294No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTTTTT43032.331377425244759No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG40482.1932177126169616No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG35681.9331523711999306No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT25771.3962258017326854No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC25291.3702192675909823No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGT25131.3615504228770812No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTCAAGA23421.268902144997264No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA21461.1627087972519763No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG21091.14266209385108No Hit
AGTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAGCACAG19761.0706023221667778No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATTACT18701.0131712259371835No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCTATC18390.9963753393040002No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAC18310.9920409169470497No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT18110.9812048610546733No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA14930.8089115723658903No Hit
GACGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAGCACAG14480.7845304466080436No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGCTT13800.7476878565739642No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGG13090.7092198581560284No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTT12440.6740026765058054No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACCTT11970.6485379451587212No Hit
GATGAACGCTGGCGGCATGCTTAACACATGCAAGTCGGACGGGAAGTGGT11940.6469125367748647No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTT11750.6366182836771073No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC11020.5970666796699337No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGCTTA10980.5948994684914585No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC9290.5033347962008788No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT8800.4767864592645569No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGAATCTTT8760.47461924808608164No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTAACCCG8660.46920122013989346No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGAA8520.4616159810152301No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTAAGA8400.4551143474798043No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG8270.4480709111497597No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA8270.4480709111497597No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAACGGA8250.44698730556052213No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATTATT7470.4047266875802546No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTC6920.37492753387621974No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTGAAA6780.36734229475155633No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAATCAAGA6740.36517508357308104No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGAACTTA6720.36409147798384345No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG6570.35596443606456124No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG6110.3310415075120957No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAACACCTT6020.32616528236052644No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGAGAGAAGAG5880.31858004323586303No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCACTTT5840.31641283205738774No Hit
GATGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAGCACAG5720.309911198521962No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGATTATC5700.3088275929327243No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAAA5610.303951367781155No Hit
GACGAACGCTGGCGGCGTGCCTAAGACATGCAAGTAGAACGCTGAAGAGA5380.2914899035049223No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATATT5090.27577762246097665No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTA5060.2741522140771202No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGTTT5000.2709013973094073No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAATTAAGA4760.2578981302385558No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT4740.25681452464931814No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTAAGA4510.2443530603730854No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATAG4460.24164404639999132No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGA4340.23514241286456558No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGGATGCG4070.22051373740985758No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGGGTTTGG4030.2183465262313823No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGCTTT3950.21401210387443176No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCCCTC3890.21076128710671888No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATACT3750.2031760479820555No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGACTTGT3740.2026342451874367No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCTGC3640.19721621724124852No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCGCTTC3630.19667441444662973No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAGCTGAATT3530.19125638650044158No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAACGAA3520.19071458370582275No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA3510.19017278091120396No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT3480.1885473725273475No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAGTTA3360.18204573899192172No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGACGACCCG3340.18096213340268408No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGACGATGAA3150.1706678803049266No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGAGGAAGG3130.169584274715689No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACATT3120.16904247192107016No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGTAGCACAG2970.16091543000178796No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCATTGA2940.15929002161793152No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGACATAT2870.1554974020555998No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGAGA2690.14574495175246116No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGAGCGAT2680.14520314895784234No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATACTT2670.14466134616322351No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCATTTT2630.14249413498474825No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACATGT2590.14032692380627299No Hit
GATGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAGCGGAGGGAGAG2510.13599250144932246No Hit
GACGAACGCTGGCGGCACGCCTAACACATGCAAGTCGAACGGACGAGGAG2490.13490889586008484No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTATTT2450.13274168468160957No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT2390.12949086791389672No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATGAAGCCT2340.12678185394080263No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTCT2310.1251564455569462No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCAAGAGA2280.12353103717308975No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAATTTT2270.12298923437847092No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGAAGCTCG2260.1224474315838521No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACGAGAGG2240.12136382599461448No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGATCCCAG2210.11973841761075804No Hit
AATGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAGCACAG2180.11811300922690159No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCTGCTT2160.11702940363766397No Hit
AGTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA2060.11161137569147582No Hit
GACGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGGAAGG2050.11106957289685701No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGGACCCT2020.10944416451300057No Hit
ATTGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA1860.10077531979909952No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT1850.1002335170044807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGCTG255.2568794E-10295.39075
ACAAACG108.42581E-4295.390662
ACGCCGG108.42581E-4295.390666
GATAAAC1100.0295.390661
AACGCCG108.42581E-4295.390665
TGAATGC206.130358E-8295.390663
CGCTGGC164700.0295.031987
CTGACAA108.460096E-4294.9905295
CTTGCGG108.460096E-4294.99059
CTGGCGG164800.0294.99059
CTAGCTA11150.0294.99059
CTAGCGA2150.0294.99059
TGAACGC93600.0294.91733
ACGCTGG164850.0294.76356
ACGAACG89500.0294.40052
GCTGGCG165150.0294.365338
GACGAAC89900.0293.090641
GCTAGCT11350.0289.792458
ACGCTAG19600.0289.362276
CGCTAGC19650.0288.6267
Produced by FastQC (version 0.11.5)