FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184569
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT3145517.042406904734815No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT178269.65817661687499No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT155508.425033456322566No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCTGGTCATCCTCTCAGACCAGCT85704.643249949883241No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT82394.463913224864414No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT63963.465370674381939No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT50492.735562310030395No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCCGATCACCCTCTCAGGTCGGCT47992.6001116113756915No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT37302.0209244239281787No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT36241.9634933276985844No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGGGACCTTCCTCTCAGAACCCCT36221.9624097221093466No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTCCGCCCTCTCAGGCCGGCT33491.81449755917841No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT32251.7473140126456774No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGTCGGCT27871.5100043886026364No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT22121.198467781696818No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGCCGGCT21211.1491637273865059No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT20871.1307424323694661No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTCCGCCCTCTCAGGCCGGCT19761.0706023221667778No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCACCCTCTCAGGTCGGCT18501.002335170044807No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT17420.9438204682259751No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT13660.7401026174493008No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTTCGGCT13510.7319755755300186No Hit
CTGGGCCGTGTCTCAGTCCCAATCTGGCCGGTCGGTCTCTCAACCCGGCT12430.6734608737111866No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT12400.6718354653273302No Hit
CTGGTCCGTGTCTCAGTACNAGTGTGGGGGGTTAACCTCTCAGTCCCCCT12380.6707518597380925No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT11640.6306584529363003No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCACCCTCTCAGGTCGGCT10600.5743109622959436No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGCCGGCT9940.5385519778511018No Hit
CTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT8760.47461924808608164No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT8250.44698730556052213No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCAACCTCTCAGTCCGGCT7620.41285372949953675No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGTCGGCT7390.400392265223304No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGATCATCCTCTCGGACCAGCT7080.3835963785901208No Hit
CTGGGCCGTGTCTCAGTCCNAATCTGGCCGGTCGGTCTCTCAACCCGGCT6920.37492753387621974No Hit
TTGGTCCGTGTCTCAGTACNAATGTGGGGGACCTTCCTCTCAGAACCCCT6830.3700513087246504No Hit
TTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT6730.36463328077846224No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCTGGTCATCCTCTCAGATCAGCT6320.34241936619909086No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGTTCATCCTCTCAGACCGGCT6300.3413357606098532No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCCGGTCACCCTCTCAGGTCGGCT6120.33158331030671456No Hit
CTGGGCCGTGTCTCAGTCCCAATGCGGCCGATCAACCTCTCAGTTCGGCT5950.3223726627981947No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT5820.31532922646815015No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGGTTAACCTCTCAGTCCCCCT5200.2817374532017836No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGAAAACCTCTCAGTCCCCCT5170.2801120448179272No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACTCTCTCAAGCCGGCT4980.2698177917201697No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT4830.26169074980088747No Hit
CTGGACCGTCTCTCAGTTCNAATGTGGCCGATCACCCTCTCAGGTCGGCT4770.2584399330331746No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGATCGGTCTCTCAACCCGGCT4600.24922928552465473No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT4600.24922928552465473No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGCCGTTCATCCTCTCAGACCGGCT4260.23080799050761505No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCTGATCATCCTCTCGGACCAGCT4130.22376455417757046No Hit
CTGGGCCGTGTCTCAGTCCNAATGCGGCCGATCAACCTCTCAGTTCGGCT3880.21021948431210008No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT3810.20642686474976837No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCACCCTCTCAGGTCGGCT3800.20588506195514958No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTCCACCCTCTCAGGCCGGCT3560.19288179488429802No Hit
TTGGACCGTCTCTCAGTTCNAATGTGGCCGATCACCCTCTCAGGTCGGCT3480.1885473725273475No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT3410.18475475296501578No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT3240.17554410545649596No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGCCGGCT3230.17500230266187713No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCGCCCTCTCAGGCCGGCT3210.17391869707263952No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCAACCTCTCAGTTCGGCT3180.17229328868878305No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCCGATCACCCTCTCAGGCCGGCT3140.1701260775103078No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT3140.1701260775103078No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCGCCCTCTCAGGCCGGCT2850.15441379646636216No Hit
CTGGTCCGTGTCTCAGTACNAGTGTGGGGGGAAAACCTCTCAGTCCCCCT2820.15278838808250572No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGCCGGCT2740.1484539657255552No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT2740.1484539657255552No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCAACCTCTCAGTCCGGCT2720.14737036013631757No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT2700.14628675454707996No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGATCGGTCTCTCAACCCGGCT2690.14574495175246116No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT2680.14520314895784234No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTCCGCCCTCTCAGGCCGGCT2620.14195233219012945No Hit
TTGGTCCGTGTCTCAGTACNAATGTGGGGGGTTAACCTCTCAGTCCCCCT2560.13870151542241654No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGGGACCTTCCTCTCAGATCCCCT2490.13490889586008484No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCATCCTCTCAGACCGGCT2480.13436709306546601No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTCCACCCTCTCAGGCCGGCT2410.13057447350313434No Hit
CTGGGCCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT2230.12082202319999566No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTGAACCTCTCAGTCCCCCT2200.11919661481613923No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAGTCTCTCAACTCGGCT2090.11323678407533226No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGTCGGCT2090.11323678407533226No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCGCCCTCTCAGGCCGGCT2070.11215317848609463No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGCCAACCTCTCAGTCCGGCT2070.11215317848609463No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGCCGATCACCCTCTCAGGTCGGCT1940.10510974215605004No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGGTCAACCTCTCAGTCCGGCT1900.10294253097757479No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCCGCT1870.10131712259371835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTA700.0292.33164
GTGTCTC171100.0292.160778
CGTGTCT171300.0291.9057
CTGGGCC103450.0291.766451
TGGACCG43300.0291.65652
TGTCTCA171450.0291.64969
GCCGTGT124500.0291.62725
CCGTGTC171800.0291.48086
GGACCGT43350.0291.32013
TTGGACC13900.0291.280061
ACCGTGT39900.0289.400975
CTGGACC29300.0289.338441
CGTATCT9750.0289.333347
GTATCTC9750.0289.333348
TGGTCCG7900.0288.63122
CTGGTCC5150.0286.65531
TTGGGCC29650.0285.9231
GCCGTAT9100.0285.906745
GACCGTG40450.0285.465974
GGCCGTA9100.0284.30054
Produced by FastQC (version 0.11.5)