FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156152
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCTTGAT103676.63904400840207No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC74924.7978892361289No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT69974.480890414467955No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTCTTA58003.7143296275423947No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA54213.4716173984322967No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATATTG49803.1892002664070906No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT47273.0271786464470516No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG43772.8030380654746656No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGCTATTT41962.687125365028946No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGTTT40132.5699318612633846No Hit
AACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGGGAGAATTG39892.554562221425278No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA39082.5026896869716686No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAGGAAAA38802.4847584404938776No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGGTCAAGG36122.313130795635022No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATTTAT25641.641989856037707No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGAATTTT20671.3237102310569189No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATATTT16381.048977918950766No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGTGAAGAGG14940.9567600799221272No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGCGAAAGAGA13120.8402069778164866No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT12680.8120293047799579No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTAACCCG11870.7601567703263487No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGATGCAT11790.7550335570469799No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTAAAG11790.7550335570469799No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTTCTT10530.674342947896921No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGACACGG10490.6717813412572365No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGTTAAACCG10150.650007684819919No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGA9640.6173472001639428No Hit
GACGAACGCTGGCGGCGTGCTTAATACATGCAAGTCGAACGGAGTTAAGG9180.5878887238075721No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCATCATGG9170.587248322147651No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA9150.5859675188278088No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCCGA9150.5859675188278088No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAATCTTT8940.5725190839694656No Hit
GATGAACGCTGGCGGCGTGCTTAACACATTCAAGTCGAACGGAGTTCAAT8400.5379373943337261No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTTTTT8370.5360161893539628No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT8190.524488959475383No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAAGTGGCCC8150.5219273528356986No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGATTATC8050.5155233362364875No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGAG7690.4924688764793278No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGAGAGAAGAG7310.4681336134023259No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGACCATA7100.45468517854398277No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAGAAGAT6790.43483272708642856No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTTTACT6720.43034991546698087No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCTGC6590.4220246938880065No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTTC6500.4162610789487166No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTACTT6190.3964086274911624No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAC6050.387443004252267No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGATCACA6020.38552179927250374No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG5830.37335416773400276No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGAAG5780.3701521594343973No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGTTAAGGCG5710.36566934781494953No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGCGGG5640.36118653619550184No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGATGCAGCGC5590.3579845278958963No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT5500.35222091295660635No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGATTACC5460.34965930631692194No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGAAGCTCG5410.3464572980173165No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAATCTT5250.3362108714585788No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC5100.32660484655976224No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTCTTA5090.32596444489984117No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAT4810.30803319842205035No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGCGATTC4790.3067523951022081No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC4660.2984271735232338No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGA4640.2971463702033916No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT4520.28946155028433834No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGT4110.26320508222757316No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGAGGTTATT4090.26192427890773096No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGGATGCG4010.25680106562836214No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCTTGAT3970.25423945898867767No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACATGT3960.2535990573287566No Hit
GACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGGGAGAATTG3940.2523182540089144No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGG3940.2523182540089144No Hit
AACGAACGCTGGCGGCAGGCTTAACACATGCAAGTTGAACGGGATTGTTA3800.24335263077001895No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA3560.2279829909319125No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCGGAT3170.20300732619498948No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTAAAC3150.20172652287514728No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCGGGA3090.1978841129156207No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTC3070.19660330959577846No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAACGCCAA2830.181233669757672No Hit
GACGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT2770.1773912597981454No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACGCCCT2700.17290844817869766No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGGGAAGAGG2660.17034684153901328No Hit
GACGAACGCTGGCGGCATGCCTTACACATGCAAGTCGAACGGACCTAGCA2660.17034684153901328No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC2650.16970643987909215No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGATGCAGCAC2600.16650443157948663No Hit
GATGAACGCTGGCGGCGTGCATAACACATTCAAGTCGAACGGACTTTCCG2590.16586402991956556No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCATCGGGG2570.16458322659972335No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG2530.16202161996003894No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT2470.15817921000051233No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCGAA2440.15625800502074902No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA2400.1536963983810646No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGT2360.1511347917413802No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGATCATG2320.1485731851016958No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCATGG2310.14793278344177468No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGACGACCGA2200.14088836518264256No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCGGGA2090.13384394692351043No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGACGAGCAC2070.13256314360366822No Hit
GACGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGGTCAAGG2030.1300015369639838No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGACCCCGGC2010.1287207336441416No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGTGAAAGTCC2010.1287207336441416No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTATAT1990.1274399303242994No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGCCGGAAGGA1960.1255187253445361No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGGTCAAGG1860.11911470874532507No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTAAACTG1840.11783390542548286No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCACGA1820.11655310210564067No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAAGTTTAA1750.11207029048619294No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTT1740.11142988882627185No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACGA1730.11078948716635073No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGCCTT1670.10694707720682413No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCGAGCAGCA1670.10694707720682413No Hit
GATGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGGGAGAATTG1640.10502587222706082No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGGACGCAATGCTTC1620.10374506890721862No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACAGAACT1620.10374506890721862No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGGAGTTATTT1590.10182386392745531No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACGC108.426389E-4295.369353
ATAAACG6250.0295.369352
CGCTGGC138000.0295.155337
TGAACGC59000.0294.86873
ACGCTGG138150.0294.834876
CTGGCGG138250.0294.777659
CGCTAGC17800.0294.539647
ACGCTAG17800.0294.539646
AACGCTG138350.0294.515355
GACGAAC85950.0293.479251
CTAGCTA9550.0293.446569
GCTGGCG138950.0293.292638
GATAAAC6300.0293.025151
GAACGCT151450.0290.78624
ACGAACG91750.0290.379462
GCTAGCG8450.0288.0098
CTAGCGA5100.0286.314829
CAGACAC3000.0285.15802295
CGAACGC94300.0282.37063
AAACGCT6700.0279.93964
Produced by FastQC (version 0.11.5)