FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156152
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT121287.766791331523132No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT74884.795327629489216No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCAGCCTCTCAGCTCGGCT74854.7934064245094525No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT70294.501383267585429No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT68774.404042215277422No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT67944.35088887750397No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT66664.268917465034069No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGATCAGCCTCTCAGCTCGGCT44122.825452123571904No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT43562.7895896306163226No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCAACCTCTCAGTCCGGCT42602.7281110712638967No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGTCGGCT40402.587222706081254No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGGGACCTTCCTCTCAGAACCCCT37522.4027870280239765No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGGACATCCTCTCAGACCGGCT29301.8763768635688305No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGTTCAGCCTCTCAGCTCGGCT27001.7290844817869768No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGTTCATCCTCTCAGACCGGCT26441.6932219888313953No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGAAGAGCCTCTCAGCCCGGCT25571.6375070444182591No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGGACATCCTCTCAGACCGGCT17551.123904913161535No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGTTCAGCCTCTCAGCTCGGCT16361.0476971156309238No Hit
TTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT15961.0220810492340797No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTACAACCTCTCAGTCCGGCT15250.9766125313796814No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT15110.9676469081407859No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGTTCATCCTCTCAGACCGGCT14020.8978431272093859No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGAAGAGCCTCTCAGCCCGGCT13960.8940007172498592No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT13110.8395665761565654No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCAACCTCTCAGTTCGGCT12050.7716840002049286No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT11320.724934679030688No Hit
CTGGACCGTATCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT11250.7204518674112403No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCGGTCTCTCAACCCGGCT9370.600056355346073No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT9350.5987755520262309No Hit
TTGGACCGTCTCTCAGTTCNAATGTGGCCGATCACCCTCTCAGGTCGGCT9190.5885291254674933No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGACCTTCCTCTCAGAACCCCT9110.5834059121881243No Hit
CTGGACCGTGTTTCAGTTCCAGTGTGGCCGATCATCCTCTCAGATCGGCT8840.5661150673702546No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT8700.5571494441313593No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT8600.5507454275321482No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTACAACCTCTCAGTCCGGCT8500.5443414109329372No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT8330.5334545827142785No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAGCCTCTCAGCCCGGCT8270.5296121727547518No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGGTCAACCTCTCAGTCCGGCT7350.47069522004201036No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGATCGGTCTCTCAACCCGGCT6960.4457195553050874No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCATCCTCTCAGACCGGCT6650.4258671038475332No Hit
CTGGACCGTATCTCAGTTCNAATGTGGCCGTTCAACCTCTCAGTCCGGCT6400.4098570623495056No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT6050.387443004252267No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGCCAACCTCTCAGTCCGGCT5950.381038987653056No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGGTTAACCTCTCAGTCCCCCT5880.3765561760336083No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT5800.3714329627542394No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT5790.37079256109431835No Hit
CTGGACCGTGTTTCAGTTCNAGTGTGGCCGATCATCCTCTCAGATCGGCT5610.3592653312157385No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTTCGGCT5500.35222091295660635No Hit
CTGGACCGTCTCTCAGTTCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT5450.34901890465700086No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGCCGATCACCCTCTCAGGTCGGCT5440.3483785029970798No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGAAAACCTCTCAGTCCCCCT5340.34197448639786876No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGATCACCCTCTCAGGTCGGCT5170.33108765817921No Hit
CTGGACCGTATCTCAGTTCCAATGTGGCCGTTCGACCTCTCAGTCCGGCT5150.3298068548593678No Hit
CTGGACCGTATCTCAGTTCCAGTGTGTCCGATCAGCCTCTCAGCTCGGAT5090.32596444489984117No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCACCCTCTCAGGTCGGCT4980.31892002664070906No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGGTCGGTCTCTCAACCCGGCT4850.3105948050617347No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCATCCTCTCAGACCGGCT4790.3067523951022081No Hit
CTGGTCCGTGTCTCAGTACNAGTGTGGGGGGTTAACCTCTCAGTCCCCCT4760.3048311901224448No Hit
CTGGTCCGTGTCTCAGTACNAGTGTGGGGGACCTTCCTCTCAGAACCCCT4630.29650596854347044No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT4560.29202315692402275No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCGACCTCTCAGTCCGGCT4430.2836979353450484No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT4210.2696090988267842No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGATCGGTCTCTCAACCCGGCT4190.2683282955069419No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCAGCCTCTCAGCCCGGCT4140.26512628720733644No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGATCACCCTCTCAGGTCGGCT3830.24527383574978223No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCAACCTCTCAGTTCGGCT3780.24207182745017675No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGCCGGCT3670.23502740919104462No Hit
CTGGACCGTATCTCAGTTCNAGTGTGTCCGATCAGCCTCTCAGCTCGGAT3460.22157897433270146No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT3460.22157897433270146No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT3440.22029817101285928No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGCCAACCTCTCAGTCCGGCT3360.21517495773349044No Hit
CTGGACCGTATCTCAGTTCNAATGTGGCCGTTCGACCTCTCAGTCCGGCT3330.21325375275372715No Hit
CTGGACCGTCTCTCAGTTCNAGTGTGGCCGATCACCCTCTCAGGTCGGCT3260.2087709411342794No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGATCGTCCTCTCAGACCAGCT3100.19852451457554177No Hit
TTGGTCCGTGTCTCAGTACNAATGTGGGGGACCTTCCTCTCAGAACCCCT3100.19852451457554177No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT3060.19596290793585736No Hit
TTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGCCGGCT3060.19596290793585736No Hit
TTGGTCCGTGTCTCAGTACNAATGTGGGGGGTTAACCTCTCAGTCCCCCT2950.18891848967672523No Hit
CTGGTCCGTGTCTCAGTACNAGTGTGGGGGGAAAACCTCTCAGTCCCCCT2870.18379527639735643No Hit
TTGGACCGTGTCTCAGTTCNAATGTGGGGGACCTTCCTCTCAGATCCCCT2810.17995286643782982No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCCGATCGACCTCTCAGTCCGGCT2790.1786720631179876No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCCGGTCACCCTCTCAGGTCGGCT2750.17611045647830317No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT2750.17611045647830317No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCACCCTCTCAGGTCGGCT2700.17290844817869766No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT2630.16842563655924997No Hit
CTGGGCCGTGTCTCAGTCCCAATGCGGCCGATCAACCTCTCAGTTCGGCT2620.16778523489932887No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGTTCACCCTCTCAGGCCGGCT2620.16778523489932887No Hit
CTGGGCCGTGTCTCAGTCCNAATGTGGCCGGTCAACCTCTCAGTCCGGCT2460.15753880834059122No Hit
CTGGGCCGTGTCTCAGTCCNAGTGTGGCCGATCACCCTCTCAGGTCGGCT2180.13960756186280035No Hit
CTGGACCGTTTCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT2080.13320354526358932No Hit
TTGGACCGTATCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT1980.1267995286643783No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT1870.11975511040524618No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT1840.11783390542548286No Hit
CTGGGCCGTGTCTCAGTCCNAATGCGGCCGATCAACCTCTCAGTTCGGCT1760.11271069214611404No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGCCACCCTCTCAGGCCGGCT1740.11142988882627185No Hit
CTGGGCCGTATCTCAGTCCNAATGTGGCCGATCAACCTCTCAGTTCGGCT1730.11078948716635073No Hit
CTGGACCGTGTCTCAGTTCNAGTGTGGCTGATCGTCCTCTCAGACCAGCT1600.1024642655873764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCT35200.0292.317027
GACCGTG43650.0292.317024
GTGTCTC113700.0292.188458
GGACCGT53050.0292.04153
CGTGTCT113700.0291.80287
TGTCTCA114000.0291.419539
CTGGGCC75100.0290.565461
GCCGTGT65750.0290.316355
CCGTATC35450.0290.255556
TGGACCG53550.0289.314672
CTGGACC37600.0289.207241
GACCGTA4550.0289.104744
GCCGTAT31000.0289.016665
CCGTGTC115250.0288.00526
ACCGTGT44400.0287.70845
TTGGGCC21550.0287.56941
GGCCGTG66800.0285.97184
GACCGTC3900.0284.82174
TTGGACC16350.0282.483731
CTGGTCC4100.0281.622471
Produced by FastQC (version 0.11.5)