FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206914
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGCGGG111395.383396000270644No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTCTTA78983.8170447625583575No Hit
GATTAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT78223.7803145268082394No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACCATTG77193.7305353915153154No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAGGAAAA70033.3844979073431474No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGAGA67513.262708178276965No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAT63943.0901727287665404No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCGAA50222.4270953149617713No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGCTATTT45672.2071971930367207No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA38301.8510105647757038No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT38211.846660931594769No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTTTAA32921.5909991590709185No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATAAAG28521.378350425780759No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG26551.2831417883758471No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGAAG24561.1869665658196158No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATATTT21931.0598606184211798No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGATGGCCC21621.0448785485757366No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG19900.961752225562311No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAGGGAAA19410.9380708893549977No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG19330.9342045487497221No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGGTCAAGG19020.919222478904279No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGATCATG18620.8998907758779009No Hit
ATTGAACGCTGGCGGAACGCTTTACACATGCAAGTCGAACGGTAACGCGG17430.8423789593744261No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGT16690.8066153087756265No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGTT16600.8022656755946915No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA15920.7694017804498487No Hit
GACGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGACTGATTC15590.7534531254530868No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCCCTC15270.7379877630319843No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTAACCCG15120.7307383743970925No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGTAA14590.7051238678871415No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTA14260.6891752128903795No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGGTGGCCC13120.6340798592652019No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA13100.6331132741138831No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGAGAGAAGAG13000.6282803483572885No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGCCGTT12390.5987995012420619No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGATCGTG12150.5872004794262351No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCACT11520.5567530471596895No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATACTG11460.5538532917057328No Hit
AACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGAGAGAATTG11430.5524034139787545No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGTTACCG10980.530655248074079No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGTTAAGGCG10720.5180896411069333No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACGAGGCC9900.47845964990285816No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA9680.46782721323835025No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC9320.45042868051461No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT9120.4407628290014209No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCTGC7950.3842175976492649No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGATTATC7810.3774515015900326No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGACCATA7770.37551833128739476No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGTGAAAGTCC7740.3740684535604164No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGCATAGA7700.3721352832577786No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCTTGAT7700.3721352832577786No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA7120.34410431386953033No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAACC7080.34217114356689254No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGACGACCGA7060.3412045584155736No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGG6680.32283944054051444No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAACC6420.31027383357336863No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGGAGGGACCC6260.3025411523628174No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTGTTT6160.29770822660622287No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAAGGGACCC6110.2952917637279256No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCATCATGA5980.2890089602443527No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAATC5680.27451018297456914No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA5600.2706438423692935No Hit
GACGAACGCTGGCGGCGTGCTTAATACATGCAAGTCGAACGGAGTTAAGG4900.23681336207313186No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGATGCAT4860.234880191770494No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA4780.2310138511652184No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTCTTA4690.22666421798428335No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAATC4480.2165150738954348No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGACACTC4310.20829910010922412No Hit
GACGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT4260.20588263723092687No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT4180.20201629662565126No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGTTT4170.20153300404999178No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGT4160.20104971147433234No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAACACCTT4120.19911654117169453No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGCGGG4090.19766666344471617No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAACGCCAA4010.19380032283944054No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGACACGG3890.1880008119315271No Hit
GATGAACGCTGGCGGCGTGCATAACACATTCAAGTCGAACGGTGCAGTCT3880.18751751935586766No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGTAGCATAA3840.18558434905322985No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGAACAGG3830.1851010564775704No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGAGCGTG3820.18461776390191093No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTGTTGA3800.18365117875059203No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTG3790.18316788617493257No Hit
GATTAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAATC3690.17833496041833805No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGTGAACGGGG3510.16963569405646792No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAAGTGGTCC3490.168669108905149No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGTGAAGAGG3480.16818581632948953No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT3390.16383618314855447No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACCATTG3350.16190301284591666No Hit
GATGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT3340.16141972027025722No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA3260.1575533796649816No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGTACGAAGCGGCCC3220.15562020936234378No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCGTCTT3130.1512705761814087No Hit
ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGAACGGCAGCATGG3070.14837082072745197No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCGCCCA3050.14740423557613308No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGATCACT3040.14692094300047362No Hit
AACGAACGCTGGCGGCAGGCTTAACACATGCAAGTTGAACGGGATTTGTG2990.14450448012217634No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTGAAGCT2940.1420880172438791No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCGGGA2880.13918826178992239No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCCGA2840.13725509148728457No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAGAAGAT2810.13580521376030621No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGTACGAAGGGGCCC2800.13532192118464675No Hit
GATGAACGCTGGCGGCGTGCCTAACACATTCAAGTCGAACGGTGAAGGGA2740.13242216573069004No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT2740.13242216573069004No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGTTT2700.13048899542805223No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGATGAAA2680.12952241027673334No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGCGATTC2670.12903911770107387No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGTTTGGA2550.12323960679316046No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT2550.12323960679316046No Hit
GATGAACGCTGGCGGCGCATTTAAGACATGCAAGTCGAACGAAGATTTAT2540.122756314217501No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGGGTGCGTT2510.12130643649052263No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG2490.12033985133920373No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGGAGTGGCCC2420.11695680330958756No Hit
GACGAACGCTGGCGGCACGCCTAACACATGCAAGTCGAACGGAGATAATT2360.11405704785563084No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAGGAAAA2320.11212387755299302No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCGCGGG2310.11164058497733356No Hit
GATGAACGCTGGCGGCGTGCTTAACACATTCAAGTCGAACGGAGTTCAAT2270.10970741467469576No Hit
GATTAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGGAACACCCC2220.1072909517963985No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAATCTT2190.10584107406942014No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGGTGGTCC2180.1053577814937607No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAAG2160.10439119634244179No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTT2160.10439119634244179No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGTTAAAC2110.10197473346414453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACGC255.293259E-10295.03
ACAAACG255.293259E-10295.02
AGAACCA108.460021E-4294.99997295
AATGCTG108.460021E-4294.999975
CGCTGGC169750.0294.652447
CTGGCGG169800.0294.305089
ACGCTGG170200.0293.873386
AACGCTG170200.0293.526735
TGAACGC84200.0293.42343
CGCTAGC37100.0293.40977
ACGCTAG37100.0293.40976
GCTGGCG172150.0290.630258
CTAGCGA10100.0290.61889
ATAAACG12900.0289.282962
GACGAAC99650.0289.22731
GATAAAC13000.0287.05771
GCTAGCG26850.0286.759778
CTAGCTA11100.0284.369359
AAACGCT13400.0283.992554
TAAACGC13200.0282.708343
Produced by FastQC (version 0.11.5)