FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206914
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT191259.242970509487034No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCAGCCTCTCAGCTCGGCT183048.846187304870623No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT160297.746696695245367No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT131516.355780662497462No Hit
CTGGACCGTCTCTCAGTTCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT116625.636158017340538No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGTTCATCCTCTCAGACCGGCT92674.478672298636148No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGGTTAACCTCTCAGTCCCCCT81623.944634002532453No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTGAGCCTCTCAGCTCGGCT79503.842175976492649No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT62323.011879331509709No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGTTCAGCCTCTCAGCTCGGCT46052.2255623109117795No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT33231.6059812289163613No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT30811.489024425606774No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT23041.113506094319379No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCAACCTCTCAGTTCGGCT22741.0990073170495955No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCT22091.067593299631731No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT21581.0429453782730989No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT19410.9380708893549977No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGAAAACCTCTCAGTCCCCCT18080.8737929767922906No Hit
TTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT16740.8090317716539239No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGACCTTCCTCTCAGAACCCCT15410.7447538590912166No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGATCGGTCTCTCAACCCGGCT15210.7350880075780276No Hit
CTGGGCCGTGTCTCAGTCCCAATCTGGCCGGTCGGTCTCTCAACCCGGCT15180.7336381298510493No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT15110.7302550818214331No Hit
CTGGTCCGTATCTCAGTACCAGTGTGGGGGATAATCCTCTCAGACCCCCT14850.7176894748542874No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT14420.6969078941009308No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGCCAACCTCTCAGTCCGGCT13790.6664604618343852No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT13420.6485786365349856No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT13220.6389127850217965No Hit
CTGGGCCGTGTTTCAGTCCCAATGTGGCCGATCAGCCTCTCAGCTCGGCT13220.6389127850217965No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGGACATCCTCTCAGACCGGCT13170.6364963221434993No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCGGTCTCTCAACCCGGCT11830.5717351170051326No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATTAACCTCTCAGTTCGGCT11740.5673854838241975No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT11620.561585972916284No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT10880.5258223223174845No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCACCCTCTCAGGTCGGCT10220.4939250123239607No Hit
CTGGACCGTCTCTCAGTTCCAGTGTGGCCGGTCATCCTCTCAGATCGGCT10150.49054196429434455No Hit
CTGGACCGTATCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT9220.4455957547580154No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCGACCTCTCAGTCCGGCT8250.3987163749190485No Hit
CTGGACCGTGTTCCAGTTCCAGTGTGGCCGGTCATCCTCTCAGACCGGCT8240.39823308234338906No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT8130.39291686401113507No Hit
TTGGACCGTTTCTCAGTTCCAATGTGGCCGTTCATCCTCTCAGATCGGCT7650.36971882037948134No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGCCACCCTCTCAGGCCGGCT7220.3489372396261249No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT6920.3344384623563413No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT6400.30930724842204976No Hit
CTGGGCCGTGTCTCAGTCCCAATGCGGCCGATCAACCTCTCAGTTCGGCT6040.29190871569830945No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT5820.2812762790338015No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGCCAACCTCTCAGTCCGGCT5780.27934310873116364No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT5760.27837652357984477No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT5280.25517847994819104No Hit
CTGGACCGTTTCTCAGTTCCAATGTGGCCGGTCATCCTCTCAGATCGGCT5280.25517847994819104No Hit
CTGGGCCGTGTCTCAGTTCCAGTGTGGCCGATCAGCCTCTCAGCTCGGCT4740.2290806808625806No Hit
CTGGACCGTATCTCAGTTCCAATGTGGCCGTCCGACCTCTCAGTCCGGCT4110.19863324859603504No Hit
CTGGACCGTTTCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT3990.19283373768812165No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT3950.19090056738548383No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCAGCCTCTCAGCCCGGCT3920.18945068965850548No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGATCGTCCTCTCAGACCAGCT3920.18945068965850548No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTAGAGTCTCTCAACTCGGCT3880.18751751935586766No Hit
CTGGACCGTGTTTCAGTTCCAGTGTGGCCGATCATCCTCTCAAATCGGCT3730.18026813072097586No Hit
CTGGACCGTCTCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT3470.16770252375383007No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT3380.163352890572895No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGCCAGCCTCTCAGCTCGGCT3350.16190301284591666No Hit
CTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT3270.15803667224064105No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCAGCT3190.15417033163536542No Hit
CTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT3190.15417033163536542No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCAGCCTCTCAGCTCGGCT3090.1493374058787709No Hit
CTGGACCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT3060.1478875281517925No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT3040.14692094300047362No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT3030.1464376504248142No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAGCCTCTCAGCTCGGCT2890.13967155436558182No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCAACCTCTCAGTCCGGCT2520.12178972906618209No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCGACCTCTCAGTCCGGCT2510.12130643649052263No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAGCCTCTCAGCTCGGCT2480.11985655876354427No Hit
CTGGACCGTATCTCAGTTCCAATGTGGCCGTTCGACCTCTCAGTCCGGCT2470.11937326618788482No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTGAGCCTCTCAGCTCGGCT2360.11405704785563084No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGATCGTCCTCTCAGACCAGCT2340.11309046270431193No Hit
CTGGGCCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT2140.10342461119112287No Hit
CTGGACCGTCTCTCAGTTCCAGTGTGGCCGATCAGCCTCTCAGCTCGGCT2140.10342461119112287No Hit
CTGGACCGTTTCTCAGTTCCAATGTGGCCGGTCACCCTCTCAGGCCGGCT2090.10100814831282563No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGTTCAACCTCTCAGTCCGGCT2080.10052485573716617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCT48600.0294.69657
GGACCGT81300.0294.637153
GTGTCTC133100.0294.33518
CGTGTCT133100.0294.33517
TGGACCG81350.0294.274752
TGTCTCA133400.0293.783729
GACCGTG58050.0293.729554
CTGGGCC91350.0293.708281
CTGGACC63300.0293.60191
CCGTATC48850.0293.188326
GGCCGTA44350.0291.67424
GCCGTAT44350.0291.67425
GCCGTGT60700.0291.355045
CGTCTCT17900.0290.87997
GACCGTC17800.0290.856724
CCGTGTC135000.0290.411136
GGCCGTG61200.0288.974674
CTGGTCC8200.0287.80491
GTATCTC50000.0286.4458
ACCGTCT18150.0285.247965
Produced by FastQC (version 0.11.5)