FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217962
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGACA177448.140868591772877No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAGGAGAA175308.042686339820703No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA122765.632174415723842No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG109935.043539699580661No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT75023.44188436516457No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGATA68473.141373266899735No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATGCCG54672.5082353804791664No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGACATCT48422.22148814931043No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGGAGGGACCC42561.9526339453666237No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGAATCTTCTT38981.7883851313531718No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA37871.737458823097604No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT35861.6452409135537387No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA35211.6154192015121902No Hit
GACGAACGCTGGCGGCACGCCTAACACATGCAAGTCGAGCGGAGATTGAG30881.41676071975849No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTACAT30381.3938209412649911No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT29461.3516117488369532No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATACTG27241.2497591323258184No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAACC26891.233701287380369No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC24801.137813013277544No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT22121.01485580055239No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC18250.8373019150127087No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTTC17780.8157385232288197No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT17620.8083977941109001No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAAGAA16450.7547187124361127No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG15420.7074627687395051No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCTGC14960.6863581725254861No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAT14880.6826878079665263No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGAATCTCTTT14720.6753470788486067No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAGGAGAA14630.6712179187197768No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA14460.6634183940319872No Hit
ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGAACGGCAGCACAG13840.6349730687000487No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCACGA13010.5968930364008405No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG12510.5739532579073416No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGAAG11800.5413787724465733No Hit
GACGAACGCTGGCGGCGTGCTTCATACATGCAAGTCGAACGAGAAGCTCA11440.5248621319312541No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGGGTGCGTT10810.4959580110294455No Hit
GATGAACGCTGGCGGCGTGCCTAACACATTCAAGTCGAACGGTGAAGGGA10630.4876996907717859No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAAG9860.4523724318917977No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGTGAAAGTCC9840.4514548407520577No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAATTTACA8990.41245721731310964No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG8650.39685816793753037No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTTTCA8630.39594057679779043No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTGAAGCT8490.38951743881961076No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGA8450.38768225654013083No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTG7940.364283682476762No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTGTTT7710.35373138436975254No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGTT7590.3482258375313128No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTC7460.3422614951230031No Hit
ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGAACGGCAGCATGG7260.33308558372560354No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTGTTGA6510.29867591598535526No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGAACAGG6010.2757361374918564No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAACC5820.26701902166432684No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGC5680.26059588368614717No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGT5410.24820840329965776No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGG5030.23077417164459862No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGA4960.2275626026555088No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCCTT4910.2252686248061589No Hit
GATTAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGTAGCA4910.2252686248061589No Hit
ATTGAACGCTGGCGGCATGCCTTACACATGCAAGTCGAACGGCAGCGCGG4890.22435103366641893No Hit
GACGAACGCTGGCGGCACGCCTAACACATGCAAGTCGAACGGACGAGGAG4860.222974646956809No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTC4650.21333993998953948No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGACATAC4560.20921077986070968No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA4470.20508161973187988No Hit
AACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGAGAGAATTG4180.19177654820565052No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA4140.1899413659261706No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAACGAGAGGAGAA3960.18168304566851104No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAGAAGAT3920.1798478633890311No Hit
GATGAACGCTGGCGGCGTGCTTAACACATTCAAGTCGAACGGAGTTCAAT3900.17893027224929117No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG3880.1780126811095512No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGACATCT3680.16883676971215167No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCGATCG3650.16746038300254174No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATGCCG3600.16516640515319184No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATACTT3440.1578256760352722No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA3370.15461410704618236No Hit
GATGAACGCTGGCGGCGTGCATAACACATTCAAGTCGAACGGACGACTCG3360.1541553114763124No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGGAA3330.15277892476670246No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG3280.15048494691735256No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTA3220.1477321734981327No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATATTT3220.1477321734981327No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCACT3210.14727337792826273No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGTACGAAGTGGCCC3200.14681458235839276No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCATCATGA3060.14039144438021306No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTCGGCC2910.13350951083216342No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTCT2870.1316743285526835No Hit
GATGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT2840.13029794184307358No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCAGGG2740.1257099861443738No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAC2640.12112203044567403No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCGCCAT2630.12066323487580405No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGAATCTTCTT2600.11928684816619411No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACGG2460.11286371018801443No Hit
GACGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT2390.1096521411989246No Hit
ATTGAACGCTGGCGGAACGCTTTACACATGCAAGTCGAACGGTAACGCGG2370.10873455005918464No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG2310.10598177663996478No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGATGGCCC2300.10552298107009479No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA2290.10506418550022482No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGAGG2280.10460538993035484No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTGTTTT2260.10368779879061489No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGGATGCG2230.10231141208100496No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTCTTA2210.101393820941265No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT2200.10093502537139502No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGGGTGCGTC2200.10093502537139502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGCT12700.0295.04
AGTGCAA108.46033E-4295.0295
TCTGGCG108.46033E-4295.08
TGAACGC132400.0294.888583
ACGCTAG69550.0294.575876
CGCTAGC69550.0294.36387
CGCTGGC148250.0294.303537
ACGCTGG148450.0294.205146
AACGCTG148500.0294.006745
TAAACGC12700.0293.83863
ATAAACG12700.0293.83862
GCTAGCG12150.0293.7868
CTAGCTA57250.0293.71189
CTGGCGG148600.0293.51119
GATAAAC12750.0292.686281
GCTGGCG149150.0292.329868
ATTGAAC4850.0291.958741
GCTAGCT57850.0291.430428
GAACGCT206550.0291.286624
ATGAACG129900.0288.75482
Produced by FastQC (version 0.11.5)