FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217962
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT4969422.799387049118653No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT209759.623237078022774No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGTTCATCCTCTCAGACCGGCT172317.905506464429579No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT151246.9388241987135375No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT120655.535368550481277No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT62282.8573788091502186No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT58542.6857892660188476No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTCCGCCCTCTCAGGCCGGCT49182.2563566126205483No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT46332.125599875207605No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT42141.9333645314320844No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATTAACCTCTCAGTTCGGCT36581.678274194584377No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCAGCCTCTCAGCTCGGCT36481.6736862388856775No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT29271.3428946330094238No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCT26501.2158082601554399No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT20200.9267670511373542No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT17680.81115056753012No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTTCGGCT17410.7987630871436305No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT16030.7354492985015736No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGAAGAGCCTCTCAGCCCGGCT15470.7097567465888549No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGGTTAACCTCTCAGTCCCCCT14450.6629595984621173No Hit
ATGGGCCGTGTCTCAGTCCCATTGTGGCTGATCATCCTCTCAAACCAGCT12640.5799176003156514No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT12330.565694937649682No Hit
CTGGACCGTATCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT11970.5491782971343628No Hit
CTGGACCGTGTTCCAGTTCCAGTGTGGCCGGTCATCCTCTCAGACCGGCT11310.5188977895229443No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGCCAACCTCTCAGTCCGGCT11250.5161450161037245No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT11160.5120158559748947No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT9410.43172663124764865No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGACCTTCCTCTCAGAACCCCT9130.4188803552912893No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGATCGTCCTCTCAGACCAGCT8460.38814105211000083No Hit
CTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT7990.3665776603261119No Hit
TTGGACCGTGTCTCAGTTCCAGTGTGGCCGTTCATCCTCTCAGACCGGCT7790.35740174892871235No Hit
TTGGGCCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT6580.3018874849744451No Hit
CTGGACCGTTTCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT6570.3014286894045751No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT6480.2972995292757453No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGATTAACCTCTCAGTTCGGCT6400.2936291647167855No Hit
CTGGGCCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT5890.27023059065341665No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGTTTTGCCTCTCAGCACAGCT5050.23169176278433856No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGCCAACCTCTCAGTCCGGCT4930.22618621594589886No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGGACATCCTCTCAGACCGGCT4900.22480982923628892No Hit
CTGGGCCGTGTCTCAGTTCCAGTGTGGCCGTTCATCCTCTCAGACCGGCT4520.20737559758122978No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT4510.2069168020113598No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCGCCCTCTCAGGCCGGCT4420.20278764188253No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT4410.20232884631266002No Hit
TTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT4170.19131775263578055No Hit
CTGGACCGTATCTCAGTTCCAATGTGGCCGTTCGACCTCTCAGTCCGGCT4000.18351822794799094No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCGCCCTCTCAGGCCGGCT3970.182141841238381No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTCCGCCCTCTCAGGCCGGCT3940.18076545452877107No Hit
CTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT3570.1637900184435819No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATTAACCTCTCAGTTCGGCT3560.16333122287371193No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGAAAACCTCTCAGTCCCCCT3510.16103724502436204No Hit
TTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT3500.16057844945449207No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT3360.1541553114763124No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGTCGGCT3350.1536965159064424No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT3350.1536965159064424No Hit
TTGGACCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT3340.15323772033657243No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGTCCGGCT3320.1523201291968325No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT3230.1481909690680027No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT2800.12846275956359365No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGTTCAACCTCTCAGTCCGGCT2720.12479239500463385No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGGGGACCTTCCTCTCAGAACCCCT2700.12387480386489388No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT2610.1197456437360641No Hit
TTGGACCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT2610.1197456437360641No Hit
TTGGGCCGTGTCTCAGTTCCAGTGTGGCCGTTCATCCTCTCAGACCGGCT2530.11607527917710428No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGTTCATCCTCTCAGTCCGGCT2490.11424009689762436No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT2360.10827575448931466No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGGGGACCTTCCTCTCAGAACCCCT2310.10598177663996478No Hit
TTGGACCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT2290.10506418550022482No Hit
CTGGACCGTTTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT2220.10185261651113497No Hit
CTGGACCGTATCTCAGTTCCAGTGTGGCCGTTCAACCTCTCAGTTCGGCT2220.10185261651113497No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGGGATCTTCCTCTCAGAACCCCT2190.10047622980152504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGCC79950.0294.6311
GGCCGTG79050.0294.626834
GACCGTG88200.0294.49834
CCGTGTC175050.0294.494456
GTGTCTC174850.0294.493878
TTGGACC59750.0294.25941
TGTCTCA175100.0293.989179
TGGACCG93200.0293.892182
GGACCGT93200.0293.892183
CGTGTCT175300.0293.8227
CGTATCT39100.0293.11387
GCCGTAT36300.0292.96835
GCCGTGT79500.0292.77365
ACCGTGT88900.0292.677155
CTGGACC33550.0292.362151
GGCCGTA36350.0292.159584
CCGTATC39350.0291.25166
TTGGGCC34200.0289.824551
TGGTCCG9750.0285.923072
GGTCCGT10000.0280.253
Produced by FastQC (version 0.11.5)