FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362476
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTC231766.393802624173739No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA192615.313731115991128No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAAGGGCAGCGGGG170354.699621492181551No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGA98422.7152142486674977No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGTGGGGCAGCGGGG91712.530098544455357No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGAGGG90542.497820545360245No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAAGGGCAGCGGGA84132.3209812511724914No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA68141.8798485968726204No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG66111.8238448890409296No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG65611.8100508723336166No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA62901.7352873017799797No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA57411.5838289983336826No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT52101.4373365409020182No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTACAGA50671.397885653119103No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAACC50101.3821604740727662No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTTTAAC49031.3526412783191162No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGGG46451.281464152109381No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTACAT39271.083382072192366No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTGTTGA38061.0500005517606683No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAACC33980.937441375428994No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGTACGCGAAGGCCT33380.9208885553802183No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG33340.9197850340436332No Hit
GATGAACGCTAGCTATAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA29200.8055705757070812No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGATGGCCC27500.7586709189022169No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT25110.6927355190412606No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGG24990.6894249550315056No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGAATTTT24900.6869420320241892No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGTACGAAGGGGCCC24860.6858385106876042No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA24810.6844591090168728No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAAG24380.6725962546485836No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTA23920.6599057592778557No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAGCACAG23770.6557675542656617No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGAGA23260.6416976572242025No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT22970.6336971275339609No Hit
GACGAACGCTGGCGGCACGCCTAACACATGCAAGTCGAACGGAGATAAAG22400.617971948487624No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG22400.617971948487624No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT19330.533276685904722No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGAGA18490.5101027378364361No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGG18440.5087233361657048No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAGGGTACTT17180.47396241406327594No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAATTTACA16890.4659618843730343No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC16750.46209955969498673No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGATTACA16350.4510643463291363No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAGTTA14830.4091305355389046No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGTTT14610.4030611681876869No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTTACA14320.3950606384974453No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGATTTTTCA13640.37630077577549964No Hit
AACGAACGCTGGCGGCAGGCTTAACACATGCAAGTTGAACGGGATTTGTA13270.36609320341208795No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTTTCA12580.347057460355996No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTG12340.34043633233648574No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTAAGGC12080.33326344364868293No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG11730.3236076319535638No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTGTTT11680.32222823028283254No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAATT11510.3175382646023461No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGGGTGCGTT11490.3169865039340536No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAAGCAAAG11400.31450358092673725No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT11380.3139518202584447No Hit
GATGAACGCTGGCGGCACGCCTAACACATGCAAGTCGAACGAAGTTATTT10980.3029166068925943No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCAAAGC10920.30126132488771673No Hit
ATTGAACGCTGGCGGCATGCCTTACACATGCAAGTCGAACGGCAGCATGG10330.28498438517308733No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGGTGAAGAGA9630.2656727617828491No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC9490.26181043710480145No Hit
GATTAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGGAACACCCC9050.24967170240236597No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC8980.24774054006334212No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGAAG8900.24553349739017205No Hit
AACGAACGCTGGCGGCAGGCTTAACACATGCAAGTTGAACGTGATTTGCT8700.24001589070724683No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTC8580.2367053266974917No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGG8540.23560180536090664No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGAGGG8390.23146360034871274No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCACGA8160.22511835266334876No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGATGTACC7760.2140831392974983No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGG7500.20691025060969553No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGCGAAAGTGG7370.20332380626579416No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCCCTC7290.2011167635926241No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTAACCCG7180.19808207991701518No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCATCATGA7150.19725443891457645No Hit
GATAAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGGTGGCCC7070.19504739624140632No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT6820.18815038788774982No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAAGGGCAGCGGGG6760.18649510588287224No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTCT6650.18346042220726339No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGA6540.1804257385316545No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTC6170.17021816616824287No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGTGGGGCAGCGGGG6140.16939052516580408No Hit
GATGAACGCTGGCGGCGTGCTTAATACATGCAAGTCGAACGAAGCACCTT6040.16663172182434147No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATAAAG5910.1630452774804401No Hit
GATTAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAATC5850.16138999547556251No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAATTTT5620.15504474779019853No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGACATCT5460.15063066244385837No Hit
AACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGAGAGAATTG5400.1489753804389808No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGGATTTAA5340.14732009843410324No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCGGGA5310.14649245743166445No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGA5300.14621657709751817No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGTTACAC5260.14511305576093314No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA5250.14483717542678687No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATGCCG5240.14456129509264062No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG5230.14428541475849435No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG5190.14318189342190932No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT5030.13876780807556915No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGACA4930.13600900473410654No Hit
ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGAACGGCAGCATGG4810.1326984407243514No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGCCAAAC4770.13159491938776635No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGAACAGG4690.12938787671459626No Hit
GACGAACGCTGGCGGCGTGCTTCATACATGCAAGTCGAACGAGAAGTTAC4670.12883611604630377No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAACT4580.1263531930389874No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAAGGGACCC4570.12607731270484115No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG4500.12414615036581732No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAAGGGCAGCGGGA4470.12331850936337853No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA4360.12028382568776967No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAC4350.12000794535362341No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGACTTGGAT4320.11918030435118462No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCATTT4200.1158697403414295No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGATGAAA4070.11228329599752812No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA3970.10952449265606551No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCTGC3870.10676568931460291No Hit
ATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGGTAACATAA3800.10483452697557906No Hit
GACGAACGCTGGCGGCGTGCTTCATACATGCAAGTCGAACGCGAATCGGT3800.10483452697557906No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGTGGGGCAGCGGGG3720.10262748430240899No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTT3710.10235160396826273No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGGG3680.10152396296582394No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA3660.10097220229753141No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCAG108.4626407E-4295.06
AGACACC800.0295.0295
ACGCTAG125600.0294.530276
TGAACGC241650.0294.450653
CGCTAGC125600.0294.41287
CTGGCGG235950.0294.312359
CGCTGGC237300.0293.694677
CTAGCTA54500.0293.376169
GCTAGCG71400.0293.140758
ACGCTGG237800.0293.13926
GCTGGCG237750.0292.766548
AACGCTG239300.0291.363375
GATAAAC18350.0290.177121
GAACGCT348050.0289.066964
ATAAACG18450.0288.604342
GCTAGCT55700.0288.11498
AAACGCT18650.0287.882024
AACGCTA128650.0287.318335
ATGAACG240950.0286.98072
ATTGAAC6250.0285.561
Produced by FastQC (version 0.11.5)