FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362476
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT5795715.989196526114831No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT3901910.764574758052948No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGACCTTCCTCTCAGAACCCCT280877.748650945166025No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT226706.25420717509573No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT204425.639545790617861No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT123403.404363323364857No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGTTCATCCTCTCAGACCGGCT93712.5852746112846092No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCAGCCTCTCAGCTCGGCT76852.1201403679140136No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT72872.010339994923802No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT59271.635142740484887No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT54271.4972025734117569No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCAACCTCTCAGTTCGGCT41231.1374546176850329No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT41211.1369028570167405No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT39391.086692636202121No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT39351.085589114865536No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGGTTAACCTCTCAGTCCCCCT37741.041172381067988No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATTAACCTCTCAGTTCGGCT32800.9048874959997351No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT32320.8916452399607147No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT31520.8695748132290139No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT29620.8171575497412242No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTTCGGCT26290.7252893984705194No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT26170.7219788344607643No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT24820.6847349893510191No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT24480.6753550579900462No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT22840.6301106831900595No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT21780.6008673677705558No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT21400.5903839150729979No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCGCCCTCTCAGGCCGGCT17170.4736865337291296No Hit
TTGGTCCGTATCTCAGTACCAATGTGGGGGGTTAACCTCTCAGTCCCCCT16680.4601683973559628No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCGTCCTCTCAGACCGGCT16600.4579613546827928No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCAACCTCTCAGTCCGGCT16580.45740959401450026No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCGACCTCTCAGTCCGGCT16090.4438914576413335No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCT15060.41547578322426865No Hit
CTGGACCGTGTCTCAGTTCCAATGTGGCTGTTCGTCCTCTCAGACCAGCT14400.3972676811706154No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT14360.39616415983403036No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT13190.3638861607389179No Hit
CTGGGCCGTGTCTCAGTACCAGTGTGGGGGACCTTCCTCTCAGAACCCCT12850.354506229377945No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCACCCTCTCAGGTCGGCT12790.35285094737306744No Hit
TTGGGCCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT12220.3371257683267306No Hit
ATGGGCCGTGTCTCAGTCCCATTGTGGCTGATCATCCTCTCAAACCAGCT12110.33409108465112175No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT10640.29353667553162144No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCTGATCGTCCTCTCAGACCAGCT9680.26705216345358035No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCTACCTCTCAGTACGGCT9470.2612586764365089No Hit
TTGGACCGTGTTTCAGTTCCAATGTGGCCGATCATCCTCTTAAATCGGCT9460.26098279610236264No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT9330.25739635175846126No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT8990.2480164203974884No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGCCAACCTCTCAGTCCGGCT8250.2276012756706651No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTTAACCTCTCAGTCCGGCT8250.2276012756706651No Hit
CTGGGCCGTGTCTCAGTCCCAATCTGGCCGGTCGGTCTCTCAACCCGGCT8220.2267736346682263No Hit
TTGGACCGTGTCTCAGTACCAGTGTGGGGGACCTTCCTCTCAGAACCCCT8130.22429071166090997No Hit
CTGGGCCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT7820.2157384213023759No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGTCCGATCAGCCTCTCAGCTCGGAT7770.21435901963164455No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACTCTCTCAAGTCGGCT7750.21380725896335204No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT7670.21160021629018197No Hit
CTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT7430.20497908827067174No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGTCGGCT7270.20056500292433155No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT7230.19946148158774651No Hit
CTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT7230.19946148158774651No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT6700.1848398238779947No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGTCCGGCT6620.1826327812048246No Hit
TTGGGCCGTGTCTCAGTACCAGTGTGGGGGACCTTCCTCTCAGAACCCCT6540.1804257385316545No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGATCGGTCTCTCAACCCGGCT6430.17739105485604564No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGGACATCCTCTCAGACCGGCT6400.17656341385360685No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTGAGCCTCTCAGCTCGGCT6300.17380461051214427No Hit
CTGGGCCGTGTCTCAGTCCCAATGCGGCCGATCAACCTCTCAGTTCGGCT5600.154492987121906No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGATCGTCCTCTCAGACCAGCT5530.1525618247828822No Hit
CTGGTCCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT5510.15201006411458964No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT5450.1503547821097121No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCGGTCTCTCAACCCGGCT5110.14097485074873922No Hit
CTGGACCGTTTCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT5020.13849192774142288No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATTAACCTCTCAGTTCGGCT4980.13738840640483782No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGGGGACCTTCCTCTCAGAACCCCT4770.13159491938776635No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGATCAACCTCTCAGTCCGGCT4550.1255255520365486No Hit
TTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT4180.11531797967313698No Hit
CTGGACCGTATCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT4050.11173153532923559No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTTAACCTCTCAGTTCGGCT3870.10676568931460291No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGCCAACCTCTCAGTCCGGCT3870.10676568931460291No Hit
TTGGTCCGTGTCTCAGTACCAGTGTGGGGGACCTTCCTCTCAGAACCCCT3860.10648980898045665No Hit
CTGGACCGTATCTCAGTTCCAATGTGGCCGTTCGACCTCTCAGTCCGGCT3810.10511040730972533No Hit
CTGGACCGTGTCTCAGTACCAGTGTGGGGGACCTTCCTCTCAGAACCCCT3810.10511040730972533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTCT314550.0294.531077
GTGTCTC314800.0294.437748
GGACCGT106100.0294.30493
GACCGTG102700.0294.28194
TGGACCG106200.0294.027772
CCGTGTC315050.0294.01686
CCGTATC43500.0293.982766
TGTCTCA315850.0293.832529
GCCGTGT164700.0293.387975
CGTATCT43600.0293.30857
GGCCGTG164900.0293.032144
TTGGACC72750.0292.972471
ACCGTGT103250.0292.857155
CTGGTCC39100.0292.736571
GCCGTAT40750.0291.380375
TTGGGCC85050.0290.66431
GTATCTC44000.0290.642068
CTGGACC34350.0290.276581
CTGGGCC121200.0289.28011
GGCCGTA41050.0289.25094
Produced by FastQC (version 0.11.5)