FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160765
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA130398.110596211862035No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG90865.651727677044133No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGGAGACCT87295.429664417006189No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACCATTG83305.181476067552017No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT78694.894722109911983No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG38722.4084844338008895No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGG38422.3898236556464405No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA35142.185799147824464No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTCTTA34672.1565639287158276No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT31641.968090069355892No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA29681.8461729854134918No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGA25741.6010947656517276No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCTGC24801.542624327434454No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGAATCTTT22511.4001803875221597No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG21791.3553945199514819No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA21311.3255372749043637No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGTTT19091.1874475165614407No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAATTTACA18681.1619444530836938No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGG17111.064286380742077No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC16441.0226106428638073No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTCTTG15840.9852890865549093No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGATGCAT15130.9411252449227133No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAT13430.8353808353808353No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC12450.7744222934096352No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGTT11990.74580910023948No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT11240.6991571548533574No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCCCTC10930.6798743507604267No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGATTACT10780.6705439616832022No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGAG10140.6307343016203776No Hit
GACGAACGCTGGCGGCGTGCTTAATACATGCAAGTCGAACGGAGTTAAGG8830.5492489036792834No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAATT8790.5467607999253569No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTACAT8410.5231238142630548No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGATGGCCC7390.45967716853792806No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATAAGC7280.45283488321463006No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTCT6800.4229776381675116No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA6770.4211115603520667No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC6740.4192454825366218No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA6640.4130252231518054No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGTGAAGAGG6530.40618293782850745No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAAAGGGCCC6460.40182875625913606No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACCTT6420.39934065250520945No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG6250.3887662115510217No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG6240.38814418561254005No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGATTATT5940.369483407458091No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAACACCTT5930.36886138151960934No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA5720.35579883681149505No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACCATTG5560.3458464217957889No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCACGA5550.34522439585730724No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAGTTA5460.33962616241097254No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGAGTTTAGT5440.33838211053400924No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGAGAGAAGAG5430.33776008459552764No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG5320.33091779927222964No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATTACT5230.32531956582589494No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGTACGAAGGGGCCC5110.31785525456411534No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGCGA4930.3066587876714459No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCGAA4880.30354865797903774No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAA4860.30230460610207444No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAACGCCAA4640.2886200354554785No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTAAGA4400.27369141293191923No Hit
GATTAACGCTGGCGGCATGCCTAAGACATGCAAGTCGTACGAAGTGACCC4390.27306938699343763No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGT4320.2687152054240662No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAACC4240.26373899791621314No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTG4140.25751873853139673No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTGTTGA3880.24134606413087428No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGATTATC3700.23014959723820483No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTGAAGCT3560.22144123409946195No Hit
GATGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGCTATTA3540.2201971822224987No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGACACGG3400.2114888190837558No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCGGGA3390.21086679314527415No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTAAGA3300.20526855969893945No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG3260.20278045594501293No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGGATGCG3160.19656019656019655No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAATTTT3040.18909588529841695No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAAGTGGTCC3030.1884738593599353No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGATATTT3030.1884738593599353No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTT3020.18785183342145367No Hit
GATGAACGCTGGCGGCGTGCTTAACACATTCAAGTCGAACGGAGTTCAAT3010.18722980748297205No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATATT2970.1847417037290455No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCCGA2850.17727739246726587No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTCAAGA2840.17665536652878425No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCATCATGA2760.17167915902093117No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGAA2740.1704351071439679No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTT2730.16981308120548627No Hit
GACGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGGAGACCT2710.168569029328523No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG2690.16732497745155975No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATATTG2690.16732497745155975No Hit
GATGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT2620.16297079588218827No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTA2420.1505302771125556No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA2400.14928622523559232No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGCGATTC2350.14617609554318417No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGAGTTCAGT2330.1449320436662209No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCGCCTT2250.13995583615836782No Hit
GATGAACGCTGGCGGCGTGCTTAACACATTCAAGTCGAACGGACGACTCG2220.1380897583429229No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGT2210.13746773240444127No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGCCTT2150.13373557677355147No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT2100.13062544708114326No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAACGGA2090.13000342114266164No Hit
ATTGAACGCTGGCGGAACGCTTTACACATGCAAGTCGAACGGTAACGCGG2080.12938139520418002No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATATTT2060.12813734332721674No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT2030.12627126551177184No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA1980.12316113581936367No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGACACAT1860.11569682455758404No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAC1860.11569682455758404No Hit
GACGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT1860.11569682455758404No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGTGAAAGTCC1860.11569682455758404No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCATCAGGT1840.11445277268062079No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGA1800.11196466892669425No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGGAGAACATT1790.1113426429882126No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATAAAG1790.1113426429882126No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGA1740.10823251329580444No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGGTGGCCC1730.10761048735732279No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGACCATA1670.103878331726433No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGTTT1670.103878331726433No Hit
GATAAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGTTTTTC1650.10263427984946973No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACATT1620.10076820203402481No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGCC108.458267E-4295.0295
TGAGCCC108.458267E-4295.0295
AGAGACA157.2288058E-6295.0295
TGAACGC100050.0294.852573
CGCTGGC104750.0294.577557
CTGGCGG104400.0294.15239
ACGCTAG56100.0293.94836
CGCTAGC56050.0293.947367
AACGCTG105100.0293.596565
CTAGCTA33550.0293.241469
ACGCTGG105300.0293.038946
GCTAGCT33850.0291.94988
GCTAGCG22600.0291.736728
AAACGCT10500.0289.380954
GAACGCT152250.0289.284064
ATAAACG10550.0288.009462
ATGAACG101800.0287.90032
AACGCTA57450.0286.784155
GATGAAC102100.0286.765441
GACGAAC50600.0286.254941
Produced by FastQC (version 0.11.5)