FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234579
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG2905712.38687180011851No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA134745.743907169866016No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAGGAAAA111384.748080603975633No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC91353.8942104791989056No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA63002.685662399447521No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA62572.667331687832244No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA61082.6038136406072154No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA56202.3957813785547724No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG49312.1020636970913853No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT47802.0376930586284367No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC46171.968206872737969No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT42901.8288082053380739No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA42551.8138878586744764No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT40851.7414176034512892No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAACC40021.7060350670776154No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG35651.519743881592129No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG35591.5171861078783693No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT28771.2264524957477012No Hit
AACGAACGCTGGCGGCAGGCTTAACACATGCAAGTTGAACGTGATTTGTT24931.0627549780670904No Hit
AACGAACGCTGGCGGCAGGCTTAACACATGCAAGTTGAACGTGATTTGCT24801.0572131350206115No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT24041.024814667979657No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTC22530.9604440295167086No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA21050.8973522779106399No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGG19480.8304238657339319No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATCA16550.7055192493786742No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGTAGCACAA16490.7029614756649146No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGAGAGAAGAG15090.6432800890105252No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTC14900.6351804722502866No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGG14190.6049134833041321No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTGAAGCT12750.5435269141739031No Hit
ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGAACGGCAGCGCGG12220.520933246369027No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGTACGCGAAAGGGA12040.5132599252277484No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT11740.5004710566589508No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG11710.499192169802071No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGATTACA11360.4842718231384736No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTG11240.47915627571095454No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTCGGCC10960.46721999838007666No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTACAT10290.4386581919097617No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGAGA9870.420753775913445No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCCCTC9420.4015704730602483No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAGGAAAA8320.3546779549746567No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTAAAT8000.34103649516793916No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGA7950.3389050170731395No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTTTCA7550.3218531923147426No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGAT7440.3171639405061834No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA7330.3124746886976243No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAA6970.297128046415067No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACGG6680.2847654734652292No Hit
ATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGGTAACATAA6620.28220769975146964No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA6590.2809288128945899No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATATTT6440.27453437861019103No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA6420.27368178737227117No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA6370.27155030927747154No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGTAGCACAG6100.2600403275655536No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGC6090.2596140319465937No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTT5680.2421359115692368No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGTGAAAGTCC5610.23915184223651734No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC5480.23360999919003833No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGAATCTTT5330.22721556490563946No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCATGC5240.22337890433500016No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGAGCGAT5220.22252631309708032No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG5070.21613187881268145No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGACGAG4890.2084585576714028No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGAG4870.20760596643348297No Hit
GATGAACGCTGGCGGCGTGCTTAATACATGCAAGTCGAACGAAGCACCTT4850.20675337519556314No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA4850.20675337519556314No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGTGAGAGA4800.20462189710076348No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG4570.19481709786468526No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCCAAGA4420.1884226635802864No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATACTT4380.1867174811044467No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTACA4320.18415970739068716No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGGTAAATG4160.17733897748732835No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAGTTA4140.17648638624940852No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAACGGA3810.162418630823731No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT3690.15730308339621193No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAACGGGAGGAAAA3670.1564504921582921No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTGAAA3660.15602419653933217No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACTTT3620.15431901406349247No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCGATCG3600.15346642282557263No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTA3520.15005605787389323No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG3410.14536680606533406No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGATTT3400.14494051044637415No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCATTGA3390.14451421482741422No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTATTT3360.14323532797053445No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTCT3330.14195644111365469No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCAATTT3330.14195644111365469No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA3070.13087275502069665No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTCT3060.13044645940173674No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT3060.13044645940173674No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT2960.12618350321213748No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGATTT2960.12618350321213748No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGACATAC2930.12490461635525772No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA2920.12447832073629779No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA2850.12149425140357832No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGCCTT2820.12021536454669855No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGATGCAC2760.117657590832939No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC2720.11595240835709932No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCCCC2640.11254204340541993No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAATCT2570.10955797407270046No Hit
GACGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG2560.10913167845374053No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCACGG2520.10742649597790084No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGATGATA2510.10700020035894092No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT2500.10657390473998098No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGGATTTAA2460.10486872226414129No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG2400.10231094855038174No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTGG108.46074E-4295.0295
CGCTGGC152150.0294.61227
ACGCTAG82450.0294.463326
TGAACGC146600.0294.396333
GCTGGCG152100.0294.030248
CGCTAGC82600.0293.92867
ACGCTGG152750.0293.64816
CTAGCTA61350.0292.836189
GCTAGCT61750.0292.37258
AACGCTG153400.0292.11545
GCTAGCG21350.0291.54578
CTGGCGG153700.0290.77759
ATGAACG139450.0290.451782
AAACGCT9450.0290.317444
AACGCTA84000.0289.029755
TAAACGC9450.0288.75663
ATAAACG9450.0288.75662
GATGAAC140050.0288.470181
ACGAACG80450.0287.666262
GATAAAC9500.0287.236821
Produced by FastQC (version 0.11.5)