FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168639
Sequences flagged as poor quality0
Sequence length301
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA2875717.052401876197084No Hit
ATTGAACGCTGGCGGAACGCTTTACACATGCAAGTCGAACGGTAACGTGG2004711.887523052200262No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA116866.9295951707493515No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGATTTTA104596.202005467299973No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGTGAATCT66183.9243591340081476No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGAACTTA49782.951867598835382No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGATTCTCTTC39312.331014771197647No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA38662.2924708993767755No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT35552.1080532972799886No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG29041.7220215964278727No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA26741.5856355884463262No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT21151.2541582907868287No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG20001.1859652867960555No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGAAG19641.1646179116337263No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGTGAAAGTCC18791.1142143869448942No Hit
AGTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA18541.0993898208599435No Hit
ATTGAACGCTGGCGGAACGCTTTACACATGCAAGTCGAACGGTAACGCGG18371.089309115922177No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGATCTTCTTC17041.0104424243502392No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTAACCCA17001.0080704937766471No Hit
AATGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA13900.8242458743232586No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGATTTACTTC13790.8177230652458802No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTCT13160.7803651587118046No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGTAAATCT13080.7756212975646203No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCAATTA11670.6920107448454984No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCGATTA11330.6718493349699655No Hit
AGTGAACGCTGGCGGAACGCTTGACACATGCAAGTCGAACGGTAACGTGG11300.6700703870397714No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACGTGG10690.6338984457924917No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCAATTG10000.5929826433980278No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG9130.5413931534223994No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT8980.5324984137714289No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATTACT8670.51411595182609No Hit
AGTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA7410.4394001387579386No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCAATCG7040.41745978095221153No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTGTTT6930.41093697187483325No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCACTTT6910.4097510065880372No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCAATCA6420.38069485706153383No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA6150.36468432568978704No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGA6040.35816151661240875No Hit
AATGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA5900.3498597596048364No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGATTTTCTTC5680.33681414145007976No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTT5410.320803610078333No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAACCACTTC5280.31309483571415864No Hit
GATGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA5170.30657202663678035No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATATT5050.299456234916004No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCATTTT4230.25083165815736574No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGAGCGAT4230.25083165815736574No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGTT3910.23185621356862884No Hit
GACGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA3850.22829831770824066No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCCCTC3670.2176246301270762No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTATGT3640.2158456821968821No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGATTTTA3610.21406673426668804No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTATGC3410.20220708139872748No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACTTT3380.2004281334685334No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGTGAATCT3310.19627725496474718No Hit
AATGAACGCTGGCGGAACGCTTAACACATGCAAGTCGAACGGTAACGTGG3240.19212637646096098No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGTTAATCT3130.1856035673835827No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACCTT3060.1814526888797965No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCCCA3020.17908075830620437No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTATTT2990.17730181037601028No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGATTTTA2910.17255794922882609No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGATTTGAA2910.17255794922882609No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGATTATT2610.15476846992688523No Hit
ATTGAACGCTGGCGGAACGCTTTACACATGCAAGTCGAACGGTAACGTTG2480.14705969556271087No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGG2390.14172285177212865No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCGTTTT2360.13994390384193456No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA2330.13816495591174047No Hit
ATTGAACGCTGGCGGAACGCTTTACACATGCAAGTCGAACGGTAACAGGA2320.13757197326834245No Hit
ATTGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA2220.13164214683436215No Hit
ATTGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGTGAATCT2210.13104916419096413No Hit
GACGAACGCTGGCGGAACGCTTTACACATGCAAGTCGAACGGTAACGTGG2040.12096845925319766No Hit
GATGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA1770.10495792788145092No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGTAACGTGG1760.10436494523805288No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGAGATTTTA1720.10199301466446077No Hit
AATGAACGCTGGCGGAACGCTTGACACATGCAAGTCGAACGGTAACGTGG1720.10199301466446077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAACGC130600.0295.03
CGAGGCA108.458633E-4295.0295
ACGCTAG14200.0295.06
AGAGACA108.458633E-4295.0295
CCAACTC157.2294315E-6294.99997295
ATTGAAC72600.0294.796841
CGCTGGC154250.0294.713137
CTGGCGG154050.0294.712749
ACGCTGG154500.0294.331736
AACGCTG154600.0294.045935
GCTGGCG155300.0292.340648
CGAACGC38000.0289.953953
CGCTAGC14500.0288.896557
CTAGCTA10600.0288.042459
AATGAAC3950.0287.531651
AACGCTA14600.0286.917825
ACGAACG38300.0286.527442
CAAACTC5000.0286.15295
CTAGCGA2800.0284.46439
GCTAGCT10850.0282.764988
Produced by FastQC (version 0.11.5)