FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210293
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGATGGCCC187798.929921585597238No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT118535.636421564198523No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAATC93914.465674083302821No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG88014.185113151650317No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC84063.997279985543979No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT68523.2583110231914523No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG66813.176995905712506No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA57642.7409376441441227No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCATCTTCCT46892.2297461161332044No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAACC44942.137018350587038No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAACC42402.0162344918756214No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAATCAAGA41971.9957868307551845No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA40181.9106674972538313No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA30141.4332383864417741No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCATCTCTTC25571.215922546161784No Hit
GATTAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT24881.183111182968525No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGTTAAGGCG22101.0509146761898873No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTCAAGA21401.0176277859938276No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGGAATGTC18160.8635570370863509No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT17990.8554730780387364No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGAA16290.7746334875625913No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTATGA16220.7713047985429853No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA15970.7594166234729639No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA15500.7370668543413238No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGACTTGT15090.7175702472264888No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG13250.6300732787111316No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTGAAGCT13080.6219893196635171No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGCGGGGCAGCGGGG13040.6200872116523137No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGTTAAGGCG12580.5982129695234744No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGAAG12560.5972619155178727No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT12180.5791918894114403No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAACACCTT10790.5130936360221214No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTTC10330.4912193938932823No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGTAAGACG9910.47124725977564635No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGG9870.46934515176444297No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCTATC9440.44889749064400625No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTCT8840.42036587047595503No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGAATCTTT8810.4189392894675524No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCCCTC8790.4179882354619507No Hit
AACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGGGAGAATTG8380.3984916283471157No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGTT8200.3899321422967003No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGGTGAAGAGA8100.3851768722686918No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCATCTCTTT7770.3694844811762636No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATTACT7150.3400018070026106No Hit
ATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGGTAACATAA6970.3314423209521953No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTTTCA6880.3271625779269876No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAATGAAGTT6760.32145625389337734No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGATCTGC6490.30861702481775427No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTACAT6410.30481280879534745No Hit
GATGAACGCTGGCGGCGTGCATAACACATTCAAGTCGAACGGACTTTCCG6320.3005330657701398No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA6200.2948267417365295No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT6150.2924491067225252No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGAGA6020.28626725568611416No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGCTTT5700.2710503915964868No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG5520.26249090554607146No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACATT5460.2596377435292663No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGAATTTT5430.2582111625208638No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGATGAAACCG5160.24537193344524066No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCACGA5050.24014113641443127No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTGTTT5030.2391900824088296No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTCTTA4900.2330082313724185No Hit
GATAAACGCTGGCGGCGTGCTTAACACATTCAAGTCGAACGGAGTATGTT4830.22967954235281252No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATACT4770.22682638033600738No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGGAAGAGG4720.22444874532200312No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAAGGGGCCC4590.218266894285592No Hit
GATTAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGGAACACCCC4320.20542766520996897No Hit
GACGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGATGGCCC4300.20447661120436725No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGTTT4260.2025745031931638No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGTTT4250.20209897619036296No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG4120.19591712515395188No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGTTT4030.19163738212874418No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCTGC4010.19068632812314248No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTAACCCA3900.1854555310923331No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATATT3800.18070026106432455No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA3780.17974920705872283No Hit
GATGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT3770.17927368005592198No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGGAGACAATT3730.17737157204471857No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCACT3700.175944991036316No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC3690.17546946403351515No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGGAGTGTC3610.17166524801110833No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGCTTA3600.17118972100830745No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACATGT3590.1707141940055066No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGGGGATATTG3480.16548339697469722No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGCTAGGA3440.1635812889634938No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTT3420.16263023495789208No Hit
GACGAACGCTGGCGGCGTGCTTAATACATGCAAGTCGAACGGAGTTAAGG3320.15787496492988354No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTAAGC3160.1502665328850699No Hit
GATGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAATC3040.14456020885145962No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAAGTTAGA2980.14170704683465452No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTA2860.13600072280104428No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGACAGTT2840.13504966879544256No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAGTTA2830.13457414179264168No Hit
GATGAACGCTGGCGGCGTGCATAACACATTCAAGTCGAACGGACGATAGC2740.130294398767434No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAACGGGG2740.130294398767434No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA2700.1283922907562306No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGTACGAAGGGGCCC2580.12268596672262033No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT2550.12125938571421778No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAACGGA2550.12125938571421778No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC2530.12030833170861607No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACCTT2490.11840622369741266No Hit
GACGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT2400.11412648067220497No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG2390.11365095366940411No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT2390.11365095366940411No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGGCCGGG2370.1126998996638024No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAATTTAAA2370.1126998996638024No Hit
GACGAACGCTGGCGGCGTGCTTCATACATGCAAGTCGAACGAGAATCTGA2300.10937121064419643No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGATTATT2280.10842015663859472No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTT2260.10746910263299302No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGG2230.10604252162459045No Hit
GACGAACGCTGGCGGCACGCCTAACACATGCAAGTCGAGCGGAGATTGAG2140.10176277859938276No Hit
GATAAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGATGGCCC2120.10081172459378104No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGT2110.10033619759098021No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGC38000.0294.611857
TGAACGC109950.0294.597533
CGCTGGC172000.0294.571237
ACGCTAG38050.0294.22476
ACGCTGG172550.0293.97426
AAACGCT37050.0293.805664
TAAACGC37000.0293.40543
ATAAACG37000.0293.40542
GATAAAC37050.0293.009431
AACGCTG173450.0292.703985
GCTAGCG25500.0292.686258
CTGGCGG173150.0292.01859
GCTGGCG173350.0291.936838
GATGAAC109400.0291.090031
ATGAACG109400.0290.95522
CTAGCTA12950.0288.166029
GAACGCT174050.0287.372864
GACGAAC61650.0282.798071
ACGAACG63100.0279.338352
AACGCTA40800.0274.75495
Produced by FastQC (version 0.11.5)