FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168156
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG2075312.341516211137277No Hit
GATGAACGCTGGCTACAGGCTTAACACATGCAAGTCGCGGGGCAGCATGC114806.826993981778824No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA113446.746116701158448No Hit
GATGAACGCTGGCTACAGGCTTAACACATGCAAGTCGTGGGGCAGCATGC103656.163919217869122No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTTCTT53253.1667023478198817No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT47642.833083565260829No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGTGAAGAGG35952.1378957634577413No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTCTTA33251.9773305739908182No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCTGC32851.9535431385142368No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT31881.8958586074835273No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAGGACAA31701.8851542615190657No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA25611.5229905563881156No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAGAAGAT24431.452817621732201No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA21391.2720331121101836No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGTGGGGCAGCATGG19191.1412022169889864No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTTTTT18141.0787601988629607No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGTTT16991.0103713218677894No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGG15070.8961916315801992No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTTACA15000.8920288303717976No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG13050.7760650824234638No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGATTACA12600.74930421751231No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCAATTA12370.7356264421132758No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGATTATC12260.7290848973572159No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAATTTACA12160.7231380384880706No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC11200.6660481933442756No Hit
ATTGAACGCTGGCGGAACGCTTTACACATGCAAGTCGAACGGTAACGCGG10990.6535597897190705No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTTC10900.6482076167368396No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGCGG10540.6267989248079164No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGCGGGGCAGCATGC10470.6226361235995148No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA10170.6047955469920788No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA9980.5934965151407027No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT9680.5756559385332667No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGTGGGGCAGCATGC9470.5631675349080616No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT9440.561383477247318No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGG8930.5310544970146769No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAGAGGAT8820.5245129522586169No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATGTTT8580.5102404909726682No Hit
GATGAACGCTGGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGC7950.4727752800970528No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGCGGGGCAGCATAA7420.44125692809058253No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA7060.41984823616165945No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG6700.3984395442327363No Hit
GACAAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGACGGAGGC5760.3425390708627703No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTACAT5630.33480815433288136No Hit
GATGAACGCTGGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG5540.3294559813506506No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGC5520.3282666095768215No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGC5350.3181569494992745No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG5300.3151835200647018No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGTT5110.3038844882133257No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCTGT4940.2937748281357787No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCAGGG4790.2848545398320607No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCC4650.27652893741525725No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCCTT4180.24857870073027427No Hit
AACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGAGAGAATTG4150.24679464306953067No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTATGA4060.2414424700872999No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGA4000.23787435476581273No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGTGAAAGTCC3990.23727966887889815No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGTTAAGGCG3930.23371155355741097No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTC3840.2283593805751802No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTT3720.2212231499322058No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCCTA3580.21289754751540238No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGCTAGGA3550.21111348985465875No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAATCTT3540.21051880396774422No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT3530.20992411808082972No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGG3430.2039772592116844No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTATTC3160.18792074026499203No Hit
GACGAACGCTGGCGGCGTGCTTAATACATGCAAGTCGAACGGAGTTAAGG3020.1795951378481886No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAAGTTAGA3010.17900045196127407No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATTACT3010.17900045196127407No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG2900.1724589072052142No Hit
GATGAACGCTGGCTACAGGCTTAACACATGCAAGTCGGGGGGCAGCATGC2870.17067484954447062No Hit
GATGAACGCTGGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA2780.1653226765622398No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG2560.1522395870501201No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTA2500.14867147172863293No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG2370.14094055519874402No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAT2360.1403458693118295No Hit
GATGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT2290.13618306810342776No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGATGAAA2280.13558838221651323No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGAATCTTT2210.1314255810081115No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGGGATACGA2180.12964152334736792No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAGGACAA2090.12428935036513714No Hit
GATGAACGCTGGCGGCGTGCATAACACATTCAAGTCGAACGGACGACTCG2080.1236946644782226No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCGAT2060.12250529270439355No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGATGCAT2040.12131592093056448No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTCTTA2040.12131592093056448No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTACTT2040.12131592093056448No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTTCTT2010.11953186326982088No Hit
GACGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT1960.11655843383524822No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC1960.11655843383524822No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTGCTTGA1950.1159637479483337No Hit
GATGAACGCTGGCTACAGGCTTAACACATGCAAGTCGTGGGGCAGCATGG1920.1141796902875901No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGGAAGG1910.11358500440067557No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCGGGA1880.11180094673993196No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTTTCA1880.11180094673993196No Hit
GATGAACGCTGGCGGCGTGCCTAACACATTCAAGTCGAACGGTGAAAGGA1840.10942220319227385No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAACACCTT1830.1088275173053593No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACATTA1770.10525940198387211No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGACGATGAA1760.1046647160969576No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGTGCTCA1740.10347534432312852No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCTGC1730.102880658436214No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGTGAAGAGG1700.10109660077547039No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAGAAGAT1690.10050191488855587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGAAC2300.0295.01
TACTTAA108.458615E-4294.99997295
ATGTACG108.458615E-4294.999972
TGAACGC109700.0294.865543
CGCTAGC55150.0294.465127
ACGCTAG55150.0294.465126
AACGCTG112800.0294.215425
CTAGCTA43650.0293.986279
ACGCTGG113050.0293.434336
CTGGCTA27650.0293.399639
ATGAACG107750.0292.946662
GATAAAC6700.0292.798521
GCTGGCT27900.0292.356638
CGCTGGC113600.0291.88387
GATGAAC108100.0291.725251
GCTAGCT44250.0291.333348
CTGGCGG86100.0290.374579
GCTAGCG11600.0289.91388
CGAACGC51350.0289.542363
GAACGCT162950.0289.387854
Produced by FastQC (version 0.11.5)