FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005103643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168156
Sequences flagged as poor quality0
Sequence length301
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT3285419.537810128690026No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGGGGATTCCCCTCTCAGGGCCCCT2227113.244249387473536No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT1946111.573182045243701No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT107946.419039463355455No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT61643.665643806941174No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGGGGACCTTCCTCTCAGAACCCCT49512.9442898261138466No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGAAGAGCCTCTCAGCCCGGCT43092.562501486714717No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGGTTAACCTCTCAGTCCCCCT32731.9464069078712625No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGTTCATCCTCTCAGACCGGCT32631.940460049002117No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT32321.9220247865077664No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT31441.8696924284592877No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCGACCTCTCAGTCCGGCT27521.6365755607887913No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCAGCCTCTCAGCTCGGCT19461.1572587359356787No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT18201.0823283141844477No Hit
TTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT16520.9824210851828065No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT15570.9259259259259258No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGGGGATTCCCCTCTCAGGGCCCCT12220.7267061538095577No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT12040.7160018078450963No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCT11600.6898356288208568No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGCCAACCTCTCAGTCCGGCT11170.6642641356835319No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT9020.5364066699969077No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT8860.526891695806275No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCGCCCTCTCAGGCCGGCT8370.497752087347463No Hit
CTGGTCCGTATCTCAGTACCAGTGTGGGGGGTAGTCCTCTCAGACCCCCT8230.4894264849306596No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT8000.47574870953162546No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT7780.46266562001950573No Hit
CTGGACCGTATCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT7000.4162801208401722No Hit
TTGGACCGTGTCTCAGTCCCAATGTGGGGGATTCCCCTCTCAGGGCCCCT6920.411522633744856No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT6390.38000428173838574No Hit
TTGGGCCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT6180.36751587811318065No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGAAAACCTCTCAGTCCCCCT5320.3163728918385309No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT5310.3157782059516164No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT4990.29674825757035134No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCGGTCTCTCAACCCGGCT4960.29496419990960776No Hit
CTGGGCCGTGTCTCAGTCCCAATGCGGCCGATCAACCTCTCAGTTCGGCT4860.2890173410404624No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGGGATTCCCCTCTCAGGGCCCCT4750.28247579628440256No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTCCACCCTCTCAGGCCGGCT4570.271771450319941No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGGACATCCTCTCAGACCGGCT4440.2640405337900521No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT4340.2580936749209068No Hit
CTGGACCGTGTTCCAGTTCCAGTGTGGCCGGTCATCCTCTCAGACCGGCT4310.25630961726016316No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT4050.24084778420038533No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGGGGACCTTCCTCTCAGAACCCCT4020.23906372653964175No Hit
CTGGGCCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT3540.21051880396774422No Hit
CTGGACCGTTTCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT3270.19446228502105187No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT3140.18673136849116295No Hit
TTGGACCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT3100.18435262494350485No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGGGGATCCCCCTCTCAGGGCCCCT2750.16353861890149624No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGCCGGCT2570.15283427293703464No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGGGGATTCCCCTCTCAGGGCCCCT2550.15164490116320561No Hit
TTGGACCGTGTCTCAGTCCCAATGTGGGGGACCTTCCTCTCAGAACCCCT2530.15045552938937654No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGGGGATTCCCCTCTCAGAACCCCT2250.13380432455576965No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGCCAACCTCTCAGTCCGGCT2230.13261495278194058No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCGCCCTCTCAGGCCGGCT2170.1290468374604534No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT1970.11715311972216275No Hit
TTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT1960.11655843383524822No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGATCGTCCTCTCAGACCAGCT1920.1141796902875901No Hit
CTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT1810.10763814553153025No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT1790.10644877375770119No Hit
TTGGACCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT1770.10525940198387211No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTCCGCCCTCTCAGGCCGGCT1750.10407003021004305No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)