FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284755
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT7224425.37058172815227No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT3500612.293375006584608No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT242968.532247019367526No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT223697.855524924935471No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT115604.059630208424786No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT97253.4152165896999174No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT95493.353409070955734No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCT75702.658425664167442No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT62152.182578005653983No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT53751.887587575284016No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCACCCTCTCAGGTCGGCT43411.5244684026619375No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT38861.3646819195448718No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT33501.176449930642131No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT28220.9910273744095801No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT24200.8498533827325244No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT22460.7887482221558884No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTCCGCCCTCTCAGGCCGGCT21740.7634633281241769No Hit
TTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT18870.6626749310811049No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT17910.6289617390388229No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGCCAACCTCTCAGTCCGGCT17610.6184263665256098No Hit
CTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT15310.5376551772576424No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT13190.46320521149760324No Hit
TTGGACCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT10210.3585538445330196No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGAAAACCTCTCAGTCCCCCT9730.3416972485118786No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGGACATCCTCTCAGACCGGCT9340.3280012642447016No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT9250.3248406524907376No Hit
CTGGACCGTGTTCCAGTTCCAGTGTGGCCGGTCATCCTCTCAGACCGGCT9180.32238239890432124No Hit
TTGGACCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT8700.30552580288318026No Hit
CTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT8430.2960439676212885No Hit
TTGGGCCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT7900.2774314761812786No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGGTTAACCTCTCAGTCCCCCT7780.2732173271759934No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCGCCCTCTCAGGCCGGCT7200.2528489403171147No Hit
CTGGGCCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT6440.22615932995030819No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGGGGACCTTCCTCTCAGAACCCCT5880.206493301258977No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT5560.19525557057821635No Hit
CTGGGCCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT5200.18261312356236062No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACTCTCTCAAGTCGGCT4880.17137539288159997No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGTCGGCT4730.1661077066249934No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT4700.1650541693736721No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT4570.16048884128461308No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCGGATCATCCTCTCAGACCCGCT4510.15838176678197047No Hit
TTGGACCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT4490.15767940861442292No Hit
TTGGACCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT4470.1569770504468754No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGGTCATCCTCTCAGACCAGCT4240.14889993152007866No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGGGATCTTCCTCTCAGAACCCCT4190.14714403610120982No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCCGCT4110.14433460343101964No Hit
TTGGACCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT4040.14187634984460323No Hit
TTGGGCCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT4020.14117399167705572No Hit
TTGGACCGTGTCTCAGTTCCAGTGTGGGGGACCTTCCTCTCAGAACCCCT3960.13906691717441308No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGCCGGCT3820.1341504100015803No Hit
TTGGACCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT3520.12361503748836719No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGTTCATCCTCTCAGACCGGCT3480.1222103211532721No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCTGGTCATCCTCTCAGACCAGCT3300.11588909764534425No Hit
CTGGACCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT3190.11202612772383277No Hit
CTGGGCCGTGTCTCAGTCCCAATGCGGCCGATCAACCTCTCAGTTCGGCT3000.10535372513213112No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGGGGACCTTCCTCTCAGAACCCCT2900.10184193429439344No Hit
TTGGGCCGTGTCTCAGTCCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCT2890.10149075521061965No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT2890.10149075521061965No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT2860.10043721795929834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCT9000.0295.07
GGACCGT119400.0294.752933
TGTCTCA272050.0294.728889
GTGTCTC272000.0294.674658
CCGTGTC272000.0294.620426
CGTGTCT271950.0294.620337
GGCCGTG130850.0294.436374
TGGACCG119600.0294.383362
ACCGTGT117650.0294.24785
TTGGACC86500.0294.14741
GCCGTGT131200.0293.763345
GACCGTG118100.0293.25154
GTCCGTG25050.0292.64474
CTGGGCC81350.0292.642881
TTGGGCC59100.0291.75551
CTGGTCC15800.0290.332281
TTGGTCC9600.0287.317721
TGGTCCG25600.0286.933622
GGTCCGT25650.0286.374273
CTGGACC34300.0285.539371
Produced by FastQC (version 0.11.5)