FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005103646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251174
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA4197116.709930167931393No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG2988211.896932007293747No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG181857.240000955512911No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT149395.947669742887401No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA115144.584073192289011No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT79403.161155215109844No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGATGGCCC64952.5858568163902316No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGG57172.276111380955035No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA56832.2625749480439854No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG44551.7736708417272489No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGACGG40401.6084467341364952No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCACGA37701.5009515316075708No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA35421.4101778050275904No Hit
AGTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA28821.1474117544013314No Hit
AATGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA26961.0733595037702948No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG23890.9511334771911106No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAC19000.7564477214998369No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAATC18730.7456982012469444No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG18090.7202178569437919No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG16210.6453693455532818No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGA15780.6282497392246013No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTCTTA15630.6222777835285499No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT15480.6163058278324987No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA14870.5920198746685564No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA14380.5725114860614554No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAAAGAGGG13640.5430498379609354No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGAG13370.532300317708043No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTGTTT12530.49885736581015555No Hit
GATGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA11660.46422002277305774No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG9930.3953434670785989No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC9530.37941825188912864No Hit
GACGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA8850.3523453860670292No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTG8670.3451790392317676No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT8320.33124447594098116No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG8050.3204949556880887No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTT7990.31810617340966824No Hit
AACGAACGCTGGCGGCAGGCTTAACACATGCAAGTTGAACGGGATTTGTA6730.2679417455628369No Hit
ATTGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG6550.26077539872757527No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAAA6540.26037726834783853No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGAGAGAAGAG6230.2480352265759991No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCACTTT5910.2352950544244229No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA5550.2209623607538997No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGTACGAAGGGGCCC5530.22016609999442618No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAACGGTAACAGGA5510.21936983923495265No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAA5510.21936983923495265No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT5490.21857357847547915No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT5420.21578666581732187No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTA4990.19866705948864133No Hit
ATTGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA4440.17676988860311973No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT4400.17517736708417272No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGAGCGAGAGA4260.16960354176785813No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGCTAGGA4000.1592521518947025No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG3970.15805776075549222No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA3810.1516876746797041No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGAGACAGTG3780.15049328354049385No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTC3770.15009515316075708No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTATGT3670.14611384936338953No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA3570.14213254556602195No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCATGG3540.14093815442681168No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG3540.14093815442681168No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAT3410.13576245949023386No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGGAAGAGG3340.13297554683207655No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC3330.1325774164523398No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCTGC3330.1325774164523398No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGCGGAGACAGTG3260.12979050379418253No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCACGA3220.1281979822752355No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGGAATGTC3190.12700359113602525No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGAATCTTT3130.1246148088576047No Hit
GATGAACGCTGGCGGCGTGCTTAATACATGCAAGTCGAACGAAGCACCTT3110.12381854809813118No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT3030.12063350506023712No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGGAATGAC2950.11744846202234308No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAACC2920.11625407088313282No Hit
ATTGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGATGGCCC2920.11625407088313282No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCAATTT2890.11505967974392253No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAAGTTAGA2770.11028211518708146No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTCGGCC2710.10789333290866093No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGAAGCACTTT2690.10709707214918741No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCATTTT2620.10431015949103012No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGTTTGTT2560.10192137721260959No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)