FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196597
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG1993610.140541310396396No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGA86794.41461466858599No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTGTTT73713.7492942415194532No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAACC68273.4725860516691505No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAC55232.8093002436456302No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT54932.7940406008230037No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGCGG51702.629745113099386No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA48312.457311149203701No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGC44902.2838598757865074No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG44062.241132875883152No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTGAAGCT43822.22892516162505No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGAGAGAAGAG36641.863711043403511No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA36091.8357350315620278No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAC21831.1103933427264912No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGTTT21731.105306795118949No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA21551.0961510094253728No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGATGCAT20821.0590192118903137No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGATTACA17930.9120179860323402No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATACTG17830.906931438424798No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA17460.8881112122768913No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTA16860.8575919266316373No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCACGA14870.7563696292415448No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATGCCG14700.747722498308723No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAGTTA14640.7446705697441975No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATCA14600.7426359507011806No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGAATCTTT14030.7136426293381893No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGG13880.7060128079268758No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT13580.6907531651042488No Hit
GATGAACGCTGGCGGCGTGCTTAACACATTCAAGTCGAACGGAGAACGGG12140.6175068795556392No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAATCTT11670.5936001058001903No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACGAGGCC11550.5874962486711395No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA11410.5803750820205802No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC10820.5503644511360803No Hit
GACGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGACTGATTC10460.532052879748928No Hit
GACGAACGCTGGCGGCACGCCTAACACATGCAAGTCGAACGGACGAGAGA10290.523405748816106No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCTCA9970.5071287964719706No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGATTATC9440.48017009415199624No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA9160.4659277608508777No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG8880.45168542754975916No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGT8680.4415123323346745No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT8180.4160795942969628No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG7910.40234591575659856No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGTGAAGAGG7500.38149107056567494No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGGGTTATGC7450.3789477967619038No Hit
GACAAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGATTGTG6900.3509717849204209No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGGCTG6830.34741120159514133No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGA6760.3438506182698617No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC6550.3331688682940228No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGG6320.3214698087966754No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTCAAGA6150.3128226778638535No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCCTT5990.3046842016917857No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCCACC5880.29908899932348915No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGAG5650.2873899398261418No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA5350.2721302970035148No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTT5330.2711129874820063No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT5320.2706043327212521No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGATGGCCC5260.2675524041567267No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA5090.2589052732239047No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGA5030.2558533446593793No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGCTTA5010.25483603513787084No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGACATCT4990.25381872561636243No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTTC4970.25280141609485396No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACATC4930.250766797051837No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAATTAAGA4910.24974948753032855No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG4720.24008504707599812No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCGAGCAGCA4650.2365244637507185No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGACACTC4630.23550715422920998No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAAG4550.23143791614317613No Hit
GACGAACGCTGGCGGCGTGCGTAACCCATGCAAGTCGAACGAGCAGATTT4420.22482540425337114No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACATT4400.22380809473186264No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGCTTT4200.21363499951677797No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGAA4030.204987868583956No Hit
ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGAACGGCAGCATGG3990.20295324954093907No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGTACGAAGGGGCCC3980.20244459478018484No Hit
AACGAACGCTGGCGGCAGGCTTAACACATGCAAGTTGAACGTGATTTGCT3940.20040997573716793No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGACCATA3780.19227149956510017No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTGTTGA3690.1876936067183121No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCGCCAT3650.18565898767529515No Hit
GACGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAACC3640.18515033291454092No Hit
GATTAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGTAGCA3560.18108109482850704No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGAGGCGG3540.1800637853069986No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA3410.17345127341719355No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGAAAAGA3360.17090799961342237No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATTACT3330.16938203533115967No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTTTCA3330.16938203533115967No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCC3280.16683876152738852No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTACAT3250.16531279724512582No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG3240.16480414248437159No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCTGC3220.16378683296286312No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTCGTTT3210.16327817820210888No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT3160.1607349043983377No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGGATGCG3150.1602262496375835No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGCGAACGTTC3030.15412239250853269No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTGTTT2940.14954449966174457No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCCTG2940.14954449966174457No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTGAAGCT2930.14903584490099034No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGGGGATGTTT2930.14903584490099034No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAACCTT2830.14394929729344802No Hit
GATGAACGCTGGCGGCGCGCTTAACACATGCAAGTCGAACGGAGCTGAAG2810.14293198777193955No Hit
GATGAACGCTGGCGGCGTGCTTAACACATTCAAGTCGAACGGAGTTCAAT2790.14191467825043108No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAAGTCTTTA2790.14191467825043108No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTAACCCA2730.13886274968590567No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGCGG2700.13733678540364297No Hit
GATGAACGCTGGCGGCGTGCTTAACACATTCAAGTCGAACGGACGACTCG2640.1342848568391176No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAATTTT2620.13326754731760912No Hit
GATGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAACC2610.13275889255685488No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGACATTG2600.13225023779610065No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGC2500.12716369018855833No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTT2480.12614638066704986No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGAACCCT2350.11953386877724481No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGCTTA2330.11851655925573636No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC2290.11648194021271943No Hit
GACGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGAGATCTGT2240.11393866640894824No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGATGCAT2170.11037808308366863No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG2130.10834346404065169No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT2120.10783480927989744No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGA2080.10580019023688052No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTG2020.10274826167235512No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCC2020.10274826167235512No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGTGAGAGA2020.10274826167235512No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATATT1990.10122229739009242No Hit
ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGAACGGCAGCACAG1990.10122229739009242No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAGAA108.4596983E-4295.0295
TGAACGC94400.0294.218753
ACGCTGG163500.0294.188086
ACGCTAG32900.0294.103336
AACGCTG163900.0293.65015
ATAAACG8400.0293.244022
CGCTAGC33100.0292.326267
CGCTGGC164450.0292.30927
GCTGGCG164650.0291.506238
GCTAGCT15600.0290.272468
CTAGCTA15500.0290.24199
TAAACGC8550.0289.824553
GATAAAC8500.0289.794131
ATGAACG94400.0289.68752
AAACGCT9700.0288.917544
GATGAAC94450.0288.90951
GCTAGCG18000.0288.444468
GAACGCT190600.0287.880374
ACGAACG94250.0287.175052
GACGAAC93950.0286.52211
Produced by FastQC (version 0.11.5)