FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338160
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG3906711.552815235391531No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT335129.91010172699314No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAGGAAAA211166.244381357937071No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA193855.732493494203927No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA157424.6551928081381595No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGATGGCCC125623.714809557605867No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAT89392.64342323160634No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTA72382.1404069079725576No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA62681.853560444759877No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAGGGAAA58481.7293588833688194No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT54441.6098888100307547No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT54081.5992429619115214No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTCT45801.3543884551691507No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA45291.3393068370002366No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGAG45131.3345753489472438No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA40821.2071208895197538No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG38031.1246155665956943No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAAGTTAGA34381.016678495386799No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT28630.8466406434823752No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGTTT26100.7718239886444287No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG25840.7641353205583156No Hit
GATTAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGGAGCGCCCC24500.724509108114502No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTACAT24460.7233262361012538No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAACC24090.7123846699787083No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT24070.7117932339720843No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTG23720.7014431038561628No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGCGAAAGTGG22490.6650697894487817No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGAGA19990.5911402886207713No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAATC18770.5550626922167022No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT18660.5518097941802697No Hit
GATGAACGCTGGCGGCGTGCTTAATACATGCAAGTCGAACGAAGCACCTT17310.5118878637331441No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC16550.4894132954814289No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAACATAA15110.44682990300449493No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA14310.4231724627395316No Hit
GACGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT13860.40986515259048967No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG11840.3501301159214573No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGATCTGC11840.3501301159214573No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATATTT11640.34421575585521647No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATGCCG9740.28802933522592855No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC9200.27206056304707826No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGATGCGT8480.2507688668086113No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTCAAGA8450.2498817127986752No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA8370.24751596877217885No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAGGAAAA8080.23894014667612964No Hit
GATGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT7820.23125147859001655No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTCGTTT7560.22356281050390348No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATACTT7550.22326709250059143No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGATTCTCTTC7250.21439555240123018No Hit
AACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGAGAGAATTG7120.21055121835817364No Hit
GACGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGATGGCCC7030.2078897563283653No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT6560.19399101017269932No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGGAA6500.19221670215282707No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTCGGCC6420.18985095812633074No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTA6180.18275372604684173No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTGAAGCT6070.17950082801040929No Hit
GACGAACGCTGGCGGCATGCTTAACACATGCAAGTCGAACGGACTGATTC5760.170333569907736No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAAGAA5440.16087059380175064No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGAGGAAAA4790.14164892358646794No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGCGAGAGA4740.14017033356990774No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGA4720.13957889756328365No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGTT4660.1378045895434114No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTATTT4550.13455169150697893No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTAACCCG4520.13366453749704282No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAACACCTT4500.13307310149041873No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT4380.12952448545067424No Hit
GATAAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGTTTTTC4270.12627158741424177No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA4240.12538443340430566No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAATTACT4200.1242015613910575No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGGGGATATTG4170.12331440738112137No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA4110.1215400993612491No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGGTTT4080.12065294535131299No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAACT4040.11947007333806482No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGATTT4020.11887863733144075No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGCGAAAGAGG3970.11740004731488052No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGCTTT3950.11680861130825644No Hit
ATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGGTAACATAA3930.11621717530163236No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGG3920.11592145729832032No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG3860.11414714927844807No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGTTT3860.11414714927844807No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGATTTACTTC3790.11207712325526377No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGAGCGAT3750.11089425124201562No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTT3750.11089425124201562No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCATTGA3720.11000709723207949No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAGCGGAGACAGTG3690.10911994322214337No Hit
GATTAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGTAGCA3590.10616276318902294No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGATATTG3560.10527560917908682No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAATCAAGA3410.1008398391294062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGGC299850.0294.754067
ACGCTGG300400.0294.459876
CTGGCGG300400.0294.067089
GCTGGCG300750.0293.8728
ATAAACG43600.0293.64682
TGAACGC111250.0293.541563
ACGCTAG37900.0293.054086
AAACGCT43850.0292.981754
TAAACGC43750.0292.639983
AACGCTG302200.0292.413125
GATAAAC44000.0290.977261
ACGAACG183600.0290.179752
GAACGCT297200.0289.143684
GACGAAC184650.0287.49121
CGCTAGC38750.0286.625827
CTAGCTA19050.0286.482949
CAAGTCC1700.0286.32352295
CGAACGC187650.0284.07413
GCTAGCG20050.0280.286778
AACGCTA39750.0279.41515
Produced by FastQC (version 0.11.5)