FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159205
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA92655.819540843566471No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG75244.725982224176376No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG66754.192707515467479No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGGAAGG62383.9182186489117803No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTTCTT56483.5476272730127825No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAC56363.540089821299582No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT46382.913225087151785No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGTGG39762.4974090009735876No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT39452.4779372507144877No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGTACGCGAAAGGGA38622.425803209698188No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATGCCG32482.04013693037279No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA25241.5853773436763923No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTACAT25071.5746992870826921No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGATTACT24501.5388963914449922No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGA23871.4993247699506926No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGTGAAGAGG21061.3228227756665933No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC17701.1117741276969944No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAATTTACA16641.045193304230395No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGTAACAGGG16321.0250934329951948No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGTAGCAGGG16191.0169278603058949No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA15991.004365440783895No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGGG15530.975471875883295No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGT15090.9478345529348953No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTTTTT13710.8611538582330956No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA13690.8598976162808957No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTTACA13480.8467070757827958No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGTTT12030.7556295342482963No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCTGC11770.7392983888696963No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG11010.6915611946860966No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC10810.6789987751640967No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA10470.6576426619766966No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGACATCT10370.6513614522156967No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTG10350.6501052102634968No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGATGGCCC9830.6174429195062969No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA9710.609905467793097No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGAGG9660.6067648629125969No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT9340.586664991677397No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG8680.5452090072547973No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC8190.5144310794258975No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTATGA8110.5094061116170975No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTTC7970.5006124179516975No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGATTATT7800.48993436135799756No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGA7750.4867937564774976No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTCTTG7220.45350334474419773No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA7110.4465940140070978No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGTACGAAGTGGCCC6580.413303602273798No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT6300.39571621494299797No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGTGG6240.3919474890863981No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTTTCA6240.3919474890863981No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAAGTAGCCT5910.37121949687509814No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGGCA5730.3599133193052982No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGATTATC5180.3253666656197984No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATCA4850.3046386734084985No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATATTT4640.29144813291039856No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAACGGA4590.28830752802989856No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAATC4300.2700920197229987No Hit
GATGAACGCTGGCGGCGTGCTTAACACATTCAAGTCGAACGGAGTTCAAT4260.26757953581859867No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGTACGCGAAAGGGA4150.2606702050814987No Hit
GATTAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGGAACACCCC4070.2556452372726987No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTC4010.25187651141609874No Hit
GACAAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGATTGTG3790.2380578499418988No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGCTTT3760.23617348701359883No Hit
GACGAACGCTGGCGGCGTGCTTAATACATGCAAGTCGAACGGAGTTAAGG3740.23491724506139885No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT3320.20853616406519898No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAGTTA3230.20288307528029897No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGG3030.19032065575829907No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTGAAA2960.18592380892559907No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGTGAAAGTCC2930.1840394459972991No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTAACCCG2910.18278320404509907No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGAAAGTT2760.17336138940359913No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGTT2730.17147702647529914No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTTCTT2710.17022078452309913No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAC2700.16959266354699915No Hit
GACGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGGAAGG2690.16896454257089916No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGTACGCGAAAGGGA2670.16770830061869918No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA2630.16519581671429917No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA2620.16456769573819918No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCGAGCAGCA2610.1639395747620992No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACGG2590.1626833328098992No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA2540.1595427279293992No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCCTT2520.15828648597719921No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGT2500.1570302440249992No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGGAGTTTAGT2470.15514588109669922No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGTTAAGGCG2470.15514588109669922No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAATTTCA2430.15263339719229924No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCATCATGA2410.15137715524009926No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTA2380.14949279231179924No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAACC2350.14760842938349927No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGATCATG2340.14698030840739926No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG2320.14572406645519928No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGAATCTTT2240.14069909864639932No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTCGTTT2240.14069909864639932No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGAGGCGG2240.14069909864639932No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATACTG2230.1400709776702993No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCGGAT2210.1388147357180993No Hit
ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGAACGGCAGCATGG2160.13567413083759933No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCGGGA2130.13378976790929933No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAACT2110.13253352595709933No Hit
AGTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGGACGCGAAAGGGA2100.13190540498099934No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA2090.13127728400489935No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACCCCTT2060.12939292107659936No Hit
GACGAACGCTGGCGGCACGCCTAACACATGCAAGTCGAACGGACTTAAAA2020.12688043717219935No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT1970.12373983229169937No Hit
GATTAACGCTGGCGGCGTGCATAACACATTCAAGTCGAACGGAGAGAGGC1920.12059922741119938No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAAAGGGCCC1830.11494613862629943No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGATGAAACCG1810.11368989667409943No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAAGTTAGA1800.11306177569799943No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACCGAAGT1800.11306177569799943No Hit
GATGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT1790.11243365472189944No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAACC1770.11117741276969945No Hit
GACGAACGCTGGCGGCACGCCTAACACATGCAAGTCGAGCGGAGATTGAG1770.11117741276969945No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGA1730.10866492886529946No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGGATGCG1670.10489620300869948No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCCCTC1640.10301184008039949No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGG1600.10049935617599949No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACGC400.0294.999973
ACAAACG400.0294.999972
CGCTAGC55350.0294.733527
ACGCTAG55400.0294.467536
CTAGCTA34200.0293.706159
CTGGCGG104050.0293.58249
TGAACGC109550.0293.518923
ACGCTGG104300.0293.302986
AACGCTG104250.0293.302155
AAACGCT7800.0293.108984
TAAACGC7450.0293.020143
ACGAACG41650.0292.5212
GCTAGCT34500.0292.007238
GCTAGCG21450.0290.874158
CGCTGGC105600.0289.692237
GAACGCT153200.0289.51214
AACGCTA56400.0289.246465
ATAAACG7550.0289.139072
GCTGGCG105900.0288.45378
ATGAACG105550.0286.615332
Produced by FastQC (version 0.11.5)