FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159205
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT2908718.27015483182061No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT1609110.107094626425049No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT158529.95697371313715No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT93375.864765553845671No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTCCGCCCTCTCAGGCCGGCT57133.5884551364592823No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT52733.312081906975283No Hit
CTGGACCGTGTTTCAGTTCCAGTGTGGCCGATCATCCTCTCAGATCGGCT45082.831569360258786No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGGGGGTCTTCCTCTCAGAACCCCT44512.795766464621086No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGACCTTCCTCTCAGAACCCCT43242.7159951006563867No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGTTAACCTCTCAGTCCCCCT42532.6713985113532868No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGACCTTCCTCTCAGAACCCCT32982.0715429791777895No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT32762.05772431770359No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT26451.6613799817844916No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCAGCCTCTCAGCTCGGCT24751.5545994158474923No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT21211.3322445903080933No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCACCCTCTCAGGTCGGCT16371.028234037875695No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCGACCTCTCAGTCCGGCT14280.8969567538707954No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT13800.8668069470179957No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT11940.7499764454633963No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT10680.6708332024747967No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT10030.6300053390282968No Hit
TTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT9700.609277346816997No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTCCGGCT7340.46104079645739765No Hit
TTGGTCCGTGTCTCAGTACCAATGTGGGGGGTTAACCTCTCAGTCCCCCT6960.4371721993655978No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGCCAACCTCTCAGTCCGGCT6960.4371721993655978No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCACCCTCTCAGGTCGGCT6570.4126754812976979No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTCCGCCCTCTCAGGCCGGCT5930.3724757388272981No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGTCCGATCAGCCTCTCAGCTCGGAT5760.3617976822335982No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT5400.3391853270939983No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGGGGACCTTCCTCTCAGAACCCCT5270.3310197544046984No Hit
CTGGTCCGTGTCTCAGTACCAGTGTGGGGGGAAAACCTCTCAGTCCCCCT5220.3278791495241984No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCAACCTCTCAGTCCGGCT4980.31280424609779844No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT4960.3115480041455984No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTTAACCTCTCAGTTCGGCT4550.2857950441254986No Hit
CTGGACCGTATCTCAGTTCCAATGTGGCCGTTCGACCTCTCAGTCCGGCT4430.27825759241229864No Hit
CTGGGCCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT4420.2776294714361986No Hit
CTGGACCGTCTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT4270.26820765679469866No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCGGTCTCTCAACCCGGCT4120.2587858421531987No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCTGGTCATCCTCTCAGACCAGCT4070.2556452372726987No Hit
TTGGGCCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT3950.24810778555949875No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCGCCCTCTCAGGCCGGCT3900.24496718067899878No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCAACCTCTCAGTCCGGCT3860.2424546967745988No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT3670.23052039822869883No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCT3380.21230488992179894No Hit
CTGGACCGTGTTCCAGTTCCAGTGTGGCCGGTCATCCTCTCAGACCGGCT3080.19346126063879904No Hit
TTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT3020.18969253478219905No Hit
CTGGGCCGTGTCTCAGTCCCAATGCGGCCGATCAACCTCTCAGTTCGGCT2920.18341132502119908No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGATCGGTCTCTCAACCCGGCT2880.1808988411167991No Hit
TTGGACCGTGTCTCAGTCCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT2630.16519581671429917No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGATCGTCCTCTCAGACCAGCT2450.15388963914449924No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCATCCTCTCAGATCGGCT2430.15263339719229924No Hit
CTGGACCGTGTGTCAGTTCCAGTGTGGCCGATCATCCTCTCAGATCGGCT2430.15263339719229924No Hit
CTGGGCCGTGTCTCAGTCCCAATCTGGCCGGTCGGTCTCTCAACCCGGCT2420.15200527621619925No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT2370.14886467133569925No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGTCGGCT2160.13567413083759933No Hit
CTGGACCGTTTCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT2100.13190540498099934No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCGCCCTCTCAGGCCGGCT2080.13064916302879936No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACTCTCTCAAGCCGGCT2080.13064916302879936No Hit
CTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACTCTCTCAAGCCGGCT2030.12750855814829937No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCATCCTCTCAGACCGGCT1990.12499607424389939No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGGCTGGTCGTCCTCTCAGACCAGCT1950.12248359033949939No Hit
CTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCT1940.12185546936339939No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGTCCGGCT1890.1187148644828994No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGATCAACCTCTCAGTTCGGCT1870.11745862253069943No Hit
CTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCACCCTCTCAGGTCGGCT1790.11243365472189944No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGGGGATCTTCCTCTCAGAACCCCT1760.11054929179359944No Hit
CTGGGCCGTGTCTCAGTCCCAGTGTGTCCGATCAGCCTCTCAGCTCGGAT1760.11054929179359944No Hit
TTGGGCCGTGTCTCAGTCCCAGTGTGGGGGGTCTTCCTCTCAGAACCCCT1740.10929304984139945No Hit
TTGGACCGTGTCTCAGTCCCAATGTGGCCGTTCACCCTCTCAGGCCGGCT1730.10866492886529946No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGTTCGCCCTCTCAGGCCGGCT1730.10866492886529946No Hit
CTGGACCGTATCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT1730.10866492886529946No Hit
TTGGGCCGTGTCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGCCGGCT1680.10552432398479947No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGTTCATCCTCTCAGACCGGCT1660.10426808203259949No Hit
TTGGACCGTGTCTCAGTTCCAGTGTGGGGGACCTTCCTCTCAGAACCCCT1640.10301184008039949No Hit
CTGGGCCGTATCTCAGTCCCAATGTGGCCGGTCACCCTCTCAGGTCGGCT1630.10238371910429948No Hit
TTGGACCGTGTCTCAGTTCCAATGTGGCCGTTCAACCTCTCAGTCCGGCT1620.1017555981281995No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGAATT108.458192E-4294.99997295
CGTGTCT126050.0294.7667
GTGTCTC126000.0294.765878
GGACCGT49700.0294.406433
CGTATCT24550.0294.39927
CCGTGTC126350.0294.066076
GACCGTG46500.0294.04844
GCCGTAT23800.0293.76055
TGTCTCA126550.0293.71799
ACCGTGT46650.0293.102875
TTGGACC37950.0293.056641
GCCGTGT70700.0292.705085
CCGTATC24750.0292.616156
GGCCGTA23900.0292.531374
TTGGGCC28650.0292.425841
TGGACCG50050.0292.052952
CTGGTCC9900.0292.02021
GGCCGTG70900.0291.671364
CTGGGCC66600.0289.684661
CTGGACC12100.0288.904941
Produced by FastQC (version 0.11.5)