FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences231437
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG2764211.943639089687474No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA141836.128233601368839No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA132755.73590221096886No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGACA121485.248944637201484No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGATTT102474.427554798930163No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAATTT82233.553018748082631No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT80863.4938233730993753No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG76923.323582659643877No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA62862.716073920764614No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT52522.2693000687012015No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGG52222.2563375778289556No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGATTACT42721.845858700207832No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGATTATT40501.7499362677532115No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG39241.6954938060897782No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA34511.491118533337366No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATATTT32701.412911505074815No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACATA32261.393899851795521No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA29741.2850149284686545No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGT27281.1787225033162372No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGAAACGATA26661.1519333555135953No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA25221.0897133993268147No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATGTTA19560.8451544048704398No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGTACGCGAAAGGGA18590.8032423510501778No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCAATCT18570.8023781849920281No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCGGTTT18290.7902798601779318No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG17950.7755890371893863No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGAATCTTT16910.7306524021656001No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGTGCTCA14260.6161503994607604No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCAAGAGA13300.5746704286695732No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGAA12350.5336225409074608No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTCT11710.5059692270466692No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCGATTT11300.4882538228545998No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCTATC10470.45239093144138576No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGAAAGTT10390.4489342672087868No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCATTGA9980.43121886301671725No Hit
ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGAACGGCAGCGCGG7890.3409135099400701No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG7820.337888928736546No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT7770.3357285135911717No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTG7730.3340001814748722No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGATTT7630.3296793511841235No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCACGA7400.3197414415154016No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA7360.3180131093991021No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG6890.29770520703258335No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACGG6090.263138564706594No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCTCA5970.2579535683576956No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCATTTT5780.24974399080527312No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGGAGATAAAT5410.23375691872950305No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTCGGCC5010.21647359756650839No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAACGGA4990.21560943150835862No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA4920.21258485030483457No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGGTTT4780.20653568789778645No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTCGTTT4610.1991902764035137No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC4600.1987581933744388No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTTTCA4600.1987581933744388No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACCTT4500.19443736308369017No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCATTTG4460.1927090309673907No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTGCTTGA4420.19098069885109123No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGGGCTAGGA4140.17888237403699495No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCGGGCAGTA4110.17758612494977033No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTCT4070.1758577928334709No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTT4050.17499362677532115No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAACATTT3980.17196904557179707No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG3970.17153696254272222No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT3830.16548780013567407No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGTGAAAGTCC3760.16246321893215002No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAATATT3670.1585744716704762No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACGAGGAG3590.15511780743787726No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCAGTTT3580.15468572440880238No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAATCT3570.15425364137972752No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTACAT3500.15122906017620347No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTT3470.14993281108897885No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTATTT3310.14301948262378097No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGAGA3200.13826656930395745No Hit
AACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGAGAGAATTG3150.1361061541585831No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTGCCTT3010.13005699175153498No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAAG2980.12876074266431037No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGATTCTCTTC2960.12789657660616066No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA2780.12011908208281302No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGGTTT2720.11752658390836383No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTTGTAAA2590.11190950453039057No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCTGCC2560.11061325544316596No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGCGAAGCGATTT2520.10888492332686649No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG2450.10586034212334242No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAGTTA2360.10197159486166861No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCGCTTT2340.10110742880351889No Hit
GATGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGA2330.10067534577444402No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGTACGCGAAAGGGA2330.10067534577444402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAACGC175800.0294.66443
CGCTGGC136000.0294.457737
GCTGGCG135800.0294.456948
ACGCTAG95300.0294.071386
CGCTAGC95250.0294.07097
AACGCTG136700.0293.59735
GCTAGCT71900.0293.153668
CTAGCTA71450.0293.142039
ACGCTGG136850.0292.952126
CTGGCGG136750.0291.76429
AAACGCT3000.0290.083344
GCTAGCG23950.0289.45728
AACGCTA96750.0289.35925
ACGAACG54250.0285.755772
GAACGCT235550.0285.481844
ATGAACG174500.0282.82812
GACGAAC54600.0282.573241
ATTGAAC8050.0282.173921
GATGAAC175950.0279.6591
CTAGCGA20850.0275.191839
Produced by FastQC (version 0.11.5)