FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences233904
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTGTTT177457.586445721321568No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA166237.106761748409604No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATAAAG110824.737841165606403No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG89293.817378069635406No Hit
GATTAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT88193.7703502291538413No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGT85823.669026609207196No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAT71083.0388535467542237No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT68462.926841781243587No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTTGAT44931.920873520760654No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAATC41161.7596962856556535No Hit
AACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGAGAGAATTG39161.6741911211437173No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGAGAGAAGAG38891.6626479239346057No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGCTATTT38471.6446918393870988No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGGAAGG37971.623315548259115No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATCTTA31621.3518366509337163No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT24951.066676927286408No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGTAG24771.058981462480334No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA24761.058553936657774No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACGA20410.8725802038443121No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTCTTA18220.7789520487037417No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGACCATA18220.7789520487037417No Hit
GATGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAAGTGAAGA17110.731496682399617No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA16250.6947294616594842No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAACATT16120.6891716259662084No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATCA15350.6562521376291127No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCTATC15140.6472740953553595No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGG14980.6404336821944046No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGATGGCCC14450.6177748135987413No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAA14140.6045215130993913No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGCACCCCTC13840.5916957384226007No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC13640.5831452219714071No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATACTG13630.5827176961488474No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGTGCCTA13210.5647616116013408No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGATTACT13140.5617689308434229No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAACACCTT12920.55236336274711No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGG12810.5476605786989535No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGTT11250.4809665503796429No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGATCACA10060.4300909774950407No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCGGGA9660.41298994459265337No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGTACGCGAAAGGGA9640.41213489294753397No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGTTT9590.4099972638347356No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGTGAAAGTCC9410.40230179902866137No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCAATAA9360.4001641699158629No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG9260.39588891169026613No Hit
GACGAACGCTGGCGGCATGCCTTACACATGCAAGTCGAACGGACCTAGCA9130.39033107599699024No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACTGT8400.3591216909501334No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTG8260.35313632943429785No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG8080.34544086462822354No Hit
GACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGGAGATTATT7710.3296224091935153No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG7660.3274847800807169No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA7650.3270572542581572No Hit
GATTAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGTAGCA7570.32363704767767976No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTC7520.32149941856488135No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCGAA6660.28473219782474857No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGACACTC6660.28473219782474857No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATATTG6640.2838771461796292No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGT6600.28216704288939054No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGAGACAGTG6520.27874683630891306No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCACCTT6360.27190642314795815No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA6310.26976879403515974No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTGTTT6250.2672036390998016No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC6140.2625008550516451No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGTTT6050.258653122648608No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGTTTACT5850.2501026061974143No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAGAATCTTT5700.24368971885901908No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGACTTGT5630.24069703810110132No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT5560.23770435734318351No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT5390.2304364183596689No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCACT5260.22487858266639305No Hit
GATGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAGCGGAGGAAGGA5240.22402353102127368No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGATGAAACCG4980.21290785963472195No Hit
GACGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT4890.2090601272316848No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATATTT4810.20563992065120734No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAACGGA4790.20478486900608797No Hit
GATGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT4570.19537930090977496No Hit
ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGAACGGCAGCATGG4500.19238662015185717No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG4490.19195909432929747No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGA4400.18811136192626035No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATAAAG4150.1774232163622683No Hit
GACGAACGCTGGCGGCGTGCTTAATACATGCAAGTCGAACGGAGTTAAGG4130.17656816471714892No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCTATCT4110.17571311307202955No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGGTGAAGAGA4050.17314795813667144No Hit
GATGAACGCTGGCGGCGTGCTTAACACATTCAAGTCGAACGGAGTTCAAT3960.16930022573363432No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGTACGCGAAAGGGG3680.1573295027019632No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCACTTA3620.1547643477666051No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAATCAAGA3530.15091661536356796No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACGCCCT3370.14407620220261305No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAGCGAAGCAGTTA3370.14407620220261305No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGGCCATA3330.14236609891237428No Hit
GACGAACGCTGGCGGCGTGCCTAATACATGCAAGTAGAACGCTGAAGAGA3270.1398009439770162No Hit
GACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGAGAGAATTG3230.1380908406867775No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGAAACCTT3210.13723578904165812No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGTAAGACG3110.1329605308160613No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTT2940.12569259183254666No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGATTATC2920.12483754018742732No Hit
GATAAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCGGAT2730.11671454955879336No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATACTT2670.11414939462343525No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAT2650.11329434297831589No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT2650.11329434297831589No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGATGAAA2620.11201176551063684No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAACGCCAA2550.10901908475271906No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGTTAAGGCA2520.10773650728504003No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGAAATATA2510.10730898146248033No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTACAT2460.10517135234968192No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGATCTGC2450.10474382652712225No Hit
GACGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGAGGAAGG2380.10175114576920447No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTC2370.10132361994664478No Hit
ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGAACGGCAGCGCGG2370.10132361994664478No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGGGTGCTCA2360.10089609412408511No Hit
GATGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAGCGGAGGGAGAG2350.10046856830152541No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGGC183050.0294.677677
ACGCTAG50700.0294.418126
TGAACGC105000.0294.157143
ACGCTGG183600.0293.875276
CGCTAGC50750.0293.837437
AACGCTG183800.0293.7165
CTAGCTA34150.0293.704259
CTGGCGG183450.0293.311529
ATAAACG14950.0292.040132
GCTGGCG184600.0291.64418
GAACGCT211750.0290.681244
AAACGCT15150.0290.1324
TAAACGC15150.0289.158423
GATAAAC15100.0289.139071
GACGAAC101450.0289.038941
ACGAACG106150.0288.608122
CGAACGC106750.0287.26233
AACGCTA52000.0287.057685
GCTAGCG16900.0286.27228
ATGAACG104300.0285.38352
Produced by FastQC (version 0.11.5)