FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005103674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005103674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129370
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG90917.027131483342351No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA84786.553296745767953No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAACC84556.535518280899745No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA72345.591713689417949No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA59544.602303470665533No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTCTTA49333.8130942258638014No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT33942.623483033160702No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGG33562.5941099172914894No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAGA32682.5260879647522607No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAAGGGCATCGGGG32202.4889850815490453No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAAGGGCATCGGGA30672.3707196413387956No Hit
AACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGAGAGAATTG26932.0816263430470743No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGTCGTT21401.6541702094766946No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT20261.5660508618690578No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAAGGGCAGCGTGG19951.5420885831336477No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGA19461.5042127231970317No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTTAAA19101.4763855607946201No Hit
GATGAACGCTGGCGGCATGCCTAAGACATGCAAGTCGAACGAGATGGCCC17941.3867202597201824No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTGAAGCT13321.0296050088892326No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGC13211.0211022648218289No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTT12530.9685398469506067No Hit
ATTGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGCGAAAGTGG11410.8819664528097704No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTACAT11040.8533663136739583No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAG10970.8479554765401562No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATTC9290.7180953853289016No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGAGG8300.6415706887222695No Hit
GATGAACGCTAGCGACAGGCCTAACACATGCAAGTCGAGGGGCAGCGGAG8100.626111154054263No Hit
GATTAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAGGAAAGTA7640.5905542243178481No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAC7340.5673649223158382No Hit
ATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGGTAGCACAA6920.5348998995130246No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGATGCCG6760.5225322717786194No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATCA6670.5155754811780165No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTGT6490.5016618999768108No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATTC6480.5008889232434104No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGACATCT6380.4931591559094071No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGACTTTTCA6250.48311045837520294No Hit
GACGAACGCTGGCGGCACGCTTAACACATGCAAGTCGAACGAGAGAAGAG5240.40503980830177017No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAATTTACA5210.40272087810156915No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCATCATGA5120.3957640875009662No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCATTTA4610.3563422740975497No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCAGTT4310.33315297209553996No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGACTTAACC4220.326196181494937No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG4010.30996367009353015No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCATAA3930.3037798562263276No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTGTTGA3870.29914199582592566No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGAAGCTCTGC3830.2960500888923243No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGATGAA3610.2790446007575172No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTCAAGA3500.2705418566901136No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGATTAACCCG3390.26203911262271007No Hit
GATGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGCGAGAGA3370.2604931591559094No Hit
GATAAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGGAAGCCGTT3350.25894720568910873No Hit
ATTGAACGCTGGCGGAACGCTTTACACATGCAAGTCGAACGGTAACGCGG3320.25662827548890776No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCGCTTT3280.2535363685553065No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGGAATGTC2950.2280281363530958No Hit
GACGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAACC2910.22493622941949448No Hit
GATGAACGCTGGCGGCGCACATAAGACATGCAAGTCGAACGAACTTAACC2870.2218443224858932No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGACTTGT2840.21952539228569218No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACCTC2810.21720646208549121No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTATGA2800.2164334853520909No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCACAG2770.21411455515188993No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAGCACCCT2690.20793074128468733No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCATCGGGA2670.20638478781788672No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCAGCGGGG2660.20561181108448637No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGGAATGAC2630.20329288088428538No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGCTTCACTTC2570.19865502048388345No Hit
GACGAACGCTGGCGGCGTGCTTCATACATGCAAGTCGAACGCGAATCGGT2400.1855144160160779No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAATCAAGA2050.15846023034706655No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTCTTA2040.15768725361366623No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCACGG1960.15150343974646363No Hit
GACGAACGCTGGCGGCGTGGATAAGACATGCAAGTCGAACGAGAGAATTG1960.15150343974646363No Hit
GATGAACGCTAGCTACAGGCTTAACACATGCAAGTCGAGGGGCATCATGC1930.14918450954626267No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGGAAGAGG1890.14609260261266135No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGATGTTT1860.1437736724124604No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGCTGAGA1800.13913581201205844No Hit
GATGAACGCTAGCGGCAGGCCTAACACATGCAAGTCGAGGGGCAGCGGGA1790.13836283527865811No Hit
GATGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTA1780.1375898585452578No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTACTT1780.1375898585452578No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAGAATCTAT1780.1375898585452578No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAGTTGATTC1660.12831413774445388No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTTGAAA1470.11362757980984772No Hit
GATGAACGCTAGCGGCAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG1470.11362757980984772No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAACTGTTT1450.11208162634304708No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGCTGCTT1420.10976269614284609No Hit
GACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGCACTTT1410.10898971940944578No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAACGGAGTCGGCC1390.10744376594264513No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGAGAGAGGAA1380.10667078920924479No Hit
GATGAACGCTAGCGACAGGCTTAACACATGCAAGTCGAGGGGCAGCATGG1370.10589781247584448No Hit
GACGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAACGGA1360.10512483574244415No Hit
GATGAACGCTGGCGGCGTGCCTAACACATGCAAGTCGAGCGAAGCATTTT1360.10512483574244415No Hit
GATGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAGCGAACCACTTC1350.10435185900904383No Hit
GATAAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGAAGTTTTTC1330.10280590554224318No Hit
GACGAACGCTGGCGGCGCGCCTAACACATGCAAGTCGAACGGAGTTTACG1310.10125995207544254No Hit
ATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGAACGGCAGCGCGG1310.10125995207544254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGAAC350.0295.01
TGCACGC108.456361E-4294.999973
CAGACCC157.225548E-6294.99997295
CGCTAGC50600.0294.70857
ACGCTAG50600.0294.70856
CGCTGGC78350.0294.623477
TGAACGC81650.0294.458043
ACGCTGG78450.0294.247926
CTGGCGG78350.0294.058729
AACGCTG78500.0293.872625
GCTAGCG29600.0293.006748
CTAGCTA21200.0292.912759
ATGAACG78950.0292.75812
GCTGGCG78750.0292.752388
AAACGCT12500.0292.639984
ATAAACG12550.0291.474122
GAACGCT117150.0290.341434
TAAACGC12600.0290.317443
ACGAACG34400.0289.425872
AACGCTA51550.0289.27745
Produced by FastQC (version 0.11.5)